LOCUS pSB1C3 5911 bp DNA circular 20-AUG-2017 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|-11904098| COMMENT VNTNAME|pSB1C3| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 5911 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 40 50 0 1 0 COMMENT (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" COMMENT "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 5911 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 2541 0 3041 0 4369 0 0 COMMENT "") COMMENT #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 4 60 0 2792 0 4875 0 5767 0 COMMENT 0 "") COMMENT #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1145 0 2170 0 0 "") COMMENT #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 1 1889 0 0 "") COMMENT #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 5891 0 0 "") COMMENT #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") COMMENT #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 2930 0 5142 0 0 "") COMMENT #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 3862 0 0 "") COMMENT #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") COMMENT #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) COMMENT (CObList COMMENT #10=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 3842 3862 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #11=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 3863 3934 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #12=(CFSignal (CObList) "Stem loop" 39 0 0 3871 3905 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #13=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 3998 4017 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #14=(CFSignal (CObList) "rep (pMB1)" 21 0 1 4102 4716 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #15=(CFSignal (CObList) "ORI" 21 0 0 4117 4117 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #16=(CFSignal (CObList) "T0 terminator" 39 0 0 4883 4988 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #17=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 5001 5660 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #18=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 5774 5793 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #19=(CFSignal (CObList) "end of terminator" 39 0 1 5850 5853 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #20=(CFSignal (CObList) "Stem loop" 39 0 1 5863 5882 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #21=(CFSignal (CObList) "Red Firefly Luciferase and LRE" 4 0 0 1219 3841 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #22=(CFSignal (CObList) "Inducible pBad/araC promoter" 30 0 0 1 1210 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #23=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #24=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #25=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. 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COMMENT (CStringList) 0 84803680 (CObList))) FEATURES Location/Qualifiers misc_feature 3842..3862 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 3863..3934 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 3871..3905 /vntifkey="39" /label=Stem\loop primer_bind complement(3998..4017) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(4102..4716) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 4117..4117 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 4883..4988 /vntifkey="39" /label=T0\terminator CDS complement(5001..5660) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 5774..5793 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(5850..5853) /vntifkey="39" /label=end\of\terminator stem_loop complement(5863..5882) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix CDS 1219..3841 /vntifkey="4" /label=Red\Firefly\Luciferase\and\LRE promoter 1..1210 /vntifkey="30" /label=Inducible\pBad/araC\promoter BASE COUNT 1552 a 1451 c 1507 g 1401 t ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtt aaggaggtaa aaaaaatggc cccgaccgtg gaacaaattg 1261 tggagctggg tacgtatctg ctggccgaaa gcccgcactg ggacgatgaa acgcaaagcc 1321 tgtatttcgt cgatatcgtt ggtcgcagcg tgaacaaata cgttccgacg accaagacgc 1381 atacccaact gaaattcgac aagaatccga gcttcatcat cccggtgaag ggttgttccg 1441 accgttttat cgtcagcctg gaacgcgaga tcaacctgct gacctgggac ggcgcatcct 1501 ctgcaccatc caagattgag aagatcgcgg ttttcgataa tacgccagag aagagcgaaa 1561 accgtctgaa cgatggtaag gcagatccgc tgggcaacct gtgggcgggt accatgaata 1621 tgggttctga ccataccacc ggcacgccgg tgcgcggtac cctgagcagc ctgagcaaca 1681 agcaagtcaa agagcacgtc agcgaggtct gcatcagcaa tggcctggca tggagcaagg 1741 acctgaagaa attctattac attgatagcg ctgttcgtca agttgaccag ttcgatttcg 1801 atgcgaaaaa cctgagcttg agcaatcgtc aaccgctgtt cacctttgac aaacacggca 1861 tcatgggtag cccggatggt cagaccatcg atgcggaggg taacctgtgg gttgccacct 1921 gtcagggcga taaagtcctg aagattgata cttctacccc ggaaaccctg ctgggtattg 1981 tggagattcc ggaacatcag gtgacgagcg tttgcatcgg cggtgcagaa ctgaacgttt 2041 tgtatgtgac gaccgcgtcc attaaactgc ctggtgcgga tgaaaccaag ccgatgaaag 2101 gcgccatcta taaagtcacc ggtctgggtg tcaaaggtct gccgggtgac cgtgttaagc 2161 tgtaataagg atccttaagg aggtaaaaaa aatggagaac atggagaacg acgagaatat 2221 tgtggttggt ccgaaaccgt tctacccgat cgaagagggc agcgcgggta cccagctgcg 2281 taaatacatg gaacgctacg cgaaactggg tgcgatcgcg ttcacgaatg cggttaccgg 2341 cgtggactat tcctatgcgg agtatttgga aaagtcgtgt tgtctgggca aggcgttaca 2401 gaattatggc ctggtggtcg atggccgtat cgcgctgtgc agcgagaact gtgaagagtt 2461 tttcattccg gtgatcgcgg gtttgtttat tggcgtgggc gttgcaccga ccaatgaaat 2521 ctacacgctg cgcgaactgg tgcactccct gggtatctct aagccgacga tcgttttcag 2581 ctccaagaaa ggtttggata aagttattac ggtgcaaaag acggtcacca cgatcaaaac 2641 gattgtgatc ctggacagca aagttgatta ccgcggttat caatgcctgg atacgttcat 2701 caagcgtaac accccgccag gttttcaagc atctagcttc aaaacggtgg aggttgaccg 2761 taaagaacaa gtcgcactga ttatgaatag ctcgggtagc acgggcctgc caaagggtgt 2821 gcagctgacc catgaaaaca ccgttacccg ttttagccac gcgcgtgatc cgatttacgg 2881 caatcaagtg tcgccgggta cggctgttct gactgttgtc ccttttcacc atggttttgg 2941 catgtttacc actctgggtt acctgatttg cggttttcgc gtcgttatgc tgaccaagtt 3001 tgatgaagaa accttcctga aaaccctgca agactacaag tgcactaatg tgattctggt 3061 cccgacgctg ttcgcgatcc tgaacaaaag cgagctgctg aataagtacg acctgagcaa 3121 tttggtggag attgcgagcg gcggtgctcc gttgagcaaa gaagtaggcg aggcagtggc 3181 acgtcgcttt aacctgcctg gcgtccgtca aggttacggt ttgactgaaa ccacgagcgc 3241 gattatcatc accccggaag gtgacgataa acctggcgct agcggtaagg tcgtgccgtt 3301 gttcaaggct aaagttattg atctggacac caagaagagc ctgggcccga atcgccgtgg 3361 tgaagtttgc gttaagggcc cgatgctgat gaaaggttat gtaaacaatc cggaagcaac 3421 gaaagagctg atcgacgaag agggctggct gcacactggc gatatcggct actacgatga 3481 agagaaacac tttttcattg ttgatcgcct gaaaagcctg atcaagtaca agggttacca 3541 agtcccgcca gcggagctgg aaagcgtact gttgcagcac ccgtcgattt tcgacgccgg 3601 tgtggctggt gtgcctgatc cggtggctgg cgaactgccg ggtgctgtcg tggtgctgga 3661 gagcggtaag aatatgaccg aaaaagaagt catggactat gtggcgagcc aagtgtccaa 3721 tgcaaaacgt ctgcgtggcg gtgttcgctt tgtggacgaa gtgccgaaag gtctgaccgg 3781 caaaattgac ggtcgtgcaa ttcgtgagat tctgaagaaa ccggtcgcga aaatgtaata 3841 atactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt 3901 tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc 3961 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 4021 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 4081 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg 4141 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 4201 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 4261 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 4321 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 4381 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 4441 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 4501 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 4561 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 4621 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 4681 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 4741 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 4801 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 4861 cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga 4921 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc 4981 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat 5041 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc 5101 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg 5161 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg 5221 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg 5281 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca 5341 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca 5401 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc 5461 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg 5521 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact 5581 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat 5641 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa 5701 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg 5761 atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 5821 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga 5881 tgatttctgg aattcgcggc cgcttctaga g //