LOCUS pSB1C3 5139 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#233=(AnlzFontColorItem 100 135 33554624)) 1 (CObList)) COMMENT (CObList) 2037630044 (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 3070..3090 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 3091..3162 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 3099..3133 /vntifkey="39" /label=Stem\loop primer_bind complement(3226..3245) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(3330..3944) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 3345..3345 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 4111..4216 /vntifkey="39" /label=T0\terminator CDS complement(4229..4888) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 5002..5021 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(5078..5081) /vntifkey="39" /label=end\of\terminator stem_loop complement(5091..5110) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..3069 /vntifkey="21" /label=BBa_K568004 misc_feature 1..136 /vntifkey="21" /label=SupD-tRNA terminator 145..224 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 233..273 /vntifkey="43" /label=TE\from\coliphage\T7 promoter 282..367 /vntifkey="29" /label=Exact\promoter\of\the\blue\light\receptor RBS 376..390 /vntifkey="32" /label=Reverse\RBS\(RBSrev)\--\corresponds\to\BBa_B0030 misc_feature 397..3069 /vntifkey="21" /label=T7ptag\(T7polymerase\with\amber\mutation) BASE COUNT 1344 a 1262 c 1262 g 1271 t ORIGIN 1 caattcggag agatgccgga gcggctgaac ggaccggtct ctaaaaccgg agtaggggca 61 actctaccgg gggttcaaat ccccctctct ccgccactac agatccttag cgaaagctaa 121 ggattttttt taagcttact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 181 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 241 gctcaccttc gggtgggcct ttctgcgttt atatactaga gattgcaaaa aattaattta 301 tcattctgta cacatatttc gtacaagttt gctattgtta cttcacttaa cattgattaa 361 cattttttac tagagtttct cctctttaat tactagatga ccatgattac cgtgcactag 421 aataccatta acattgctaa gaacgacttc tctgacatcg aactggctgc tatcccgttc 481 aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc ccttgagcat 541 gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagcgtca acttaaagct 601 ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact ccctaagatg 661 attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa gcgcccgaca 721 gccttccagt tcctgtagga aatcaagccg gaagccgtag cgtacatcac cattaagacc 781 actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc aagcgcaatc 841 ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttga agctaagcac 901 ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta caagaaagca 961 tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg cgaggcgtgg 1021 tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga gatgctcatt 1081 gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg tcaagactct 1141 gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc aggtgcgctg 1201 gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg gactggcatt 1261 actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg tactcacagt 1321 aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa agcgattaac 1381 attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc caacgtaatc 1441 accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga agaactcccg 1501 atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa acgtgctgcc 1561 gctgctgtgt accgcaagga caaggctcgc aagtctcgcc gtatcagcct tgagttcatg 1621 cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta caacatggac 1681 tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc aaggtaacga tatgaccaaa 1741 ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta ctggctgaaa 1801 atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg catcaagttc 1861 attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga gaacacttgg 1921 tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta cgctggggta 1981 cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg gtcttgctct 2041 ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc ggttaacttg 2101 cttcctagtg aaaccgttca ggacatctac gggattgttg ctaagaaagt caacgagatt 2161 ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac cgatgagaac 2221 actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg tcaatggctg 2281 gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc ttacgggtcc 2341 aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc tattgattcc 2401 ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc taagctgatt 2461 tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg gcttaagtct 2521 gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat tcttcgcaag 2581 cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga atacaagaag 2641 cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca gcctaccatt 2701 aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat cgctcctaac 2761 tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc acacgagaag 2821 tacggaatcg aatcttttgc actgattcac gactccttcg gtaccattcc ggctgacgct 2881 gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc ttgtgatgta 2941 ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt ggacaaaatg 3001 ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc ggacttcgcg 3061 ttcgcataat actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc 3121 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac 3181 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 3241 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 3301 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc 3361 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 3421 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 3481 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 3541 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 3601 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 3661 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 3721 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 3781 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 3841 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 3901 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 3961 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 4021 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 4081 tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg 4141 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa 4201 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact 4261 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct 4321 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa 4381 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc 4441 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt 4501 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt 4561 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag 4621 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat 4681 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt 4741 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt 4801 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg 4861 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata 4921 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta 4981 cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta 5041 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc 5101 gctaaggatg atttctggaa ttcgcggccg cttctagag //