LOCUS pSB1C3 2193 bp DNA circular 20-AUG-2017 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|-11904098| COMMENT VNTNAME|pSB1C3| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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Location/Qualifiers misc_feature 124..144 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 145..216 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 153..187 /vntifkey="39" /label=Stem\loop primer_bind complement(280..299) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(384..998) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 399..399 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1165..1270 /vntifkey="39" /label=T0\terminator CDS complement(1283..1942) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2056..2075 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2132..2135) /vntifkey="39" /label=end\of\terminator stem_loop complement(2145..2164) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..123 /vntifkey="21" /label=BBa_K844006 BASE COUNT 585 a 541 c 533 g 534 t ORIGIN 1 atgggcggtt atggtccggg cgccggccag caaggtccgg gcagccaggg tccgggcagc 61 ggtggccaac agggtccggg tggtcagggg ccgtatggtc cgagcgctgc ggcagcggct 121 gcatactagt agcggccgct gcagtccggc aaaaaagggc aaggtgtcac caccctgccc 181 tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc 241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga 421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 1021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 1081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 1141 aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc 1201 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag 1261 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta 1321 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg 1381 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg 1441 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat 1501 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac 1561 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt 1621 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa 1681 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat 1741 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta 1801 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa 1861 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat 1921 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa 1981 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc 2041 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 2101 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag 2161 gatgatttct ggaattcgcg gccgcttcta gag //