LOCUS 20L-5-\BBa 7437 bp DNA circular 14-SEP-2017 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-11940093| COMMENT VNTDBDATE|-11940093| COMMENT VNTNAME|20L-5- BBa_K1450012| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "20L-5- BBa_K1450012" 0 7437 COMMENT (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) COMMENT (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" COMMENT "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 1 0 0 0 4294967295 1 7437 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 66 0 3022 0 0 "") COMMENT #1=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 1 5895 0 0 "") COMMENT #2=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 4663 0 6401 0 7293 0 0 "") COMMENT #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") COMMENT #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 7417 0 0 "") COMMENT #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") COMMENT #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 6668 0 0 "") COMMENT #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 5388 0 0 "") COMMENT #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") COMMENT #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) COMMENT (CObList COMMENT #10=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 5368 5388 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "5368..5388") COMMENT #11=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 5389 5460 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "5389..5460") COMMENT #12=(CFSignal (CObList) "Stem loop" 39 0 0 5397 5431 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5397..5431") COMMENT #13=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 5524 5543 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(5524..5543)") COMMENT #14=(CFSignal (CObList) "rep (pMB1)" 21 0 1 5628 6242 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 COMMENT "complement(5628..6242)") COMMENT #15=(CFSignal (CObList) "ORI" 21 0 0 5643 5643 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "5643..5643") COMMENT #16=(CFSignal (CObList) "T0 terminator" 39 0 0 6409 6514 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "6409..6514") COMMENT #17=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 6527 7186 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(6527..7186)") COMMENT #18=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 7300 7319 0 (CStringList) (CStringList) 1 1 1 1 "7300..7319") COMMENT #19=(CFSignal (CObList) "end of terminator" 39 0 1 7376 7379 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(7376..7379)") COMMENT #20=(CFSignal (CObList) "Stem loop" 39 0 1 7389 7408 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(7389..7408)") COMMENT #21=(CFSignal (CObList) "BBa_K1450012" 21 0 0 1 5367 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1..5367") COMMENT #22=(CFSignal (CObList) "Pbad promoter" 29 0 0 1 130 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #23=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 3095 3106 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #24=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 139 150 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #25=(CFSignal (CObList) "T1 from E. coli rrnB" 43 0 0 5239 5318 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #26=(CFSignal (CObList) "T1 from E. coli rrnB" 43 0 0 2820 2899 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #27=(CFSignal (CObList) "TE from coliphageT7" 43 0 0 5327 5367 0 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(LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (7293)" "@N (@S)" 5 -4.81024 COMMENT 17.4233 15.7422 7.04245 3.79641 0.799245 COMMENT #280#)) (CObjectList))) (CObjectList)) COMMENT #282=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 1672 100) COMMENT (CObjectList) (CObjectList)) COMMENT #283=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -839 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 10 COMMENT (CStringList) 1888921824 0 (CObList))) FEATURES Location/Qualifiers misc_feature 5368..5388 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 5389..5460 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 5397..5431 /vntifkey="39" /label=Stem\loop primer_bind complement(5524..5543) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(5628..6242) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 5643..5643 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 6409..6514 /vntifkey="39" /label=T0\terminator CDS complement(6527..7186) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 7300..7319 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(7376..7379) /vntifkey="39" /label=end\of\terminator stem_loop complement(7389..7408) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..5367 /vntifkey="21" /label=BBa_K1450012 promoter 1..130 /vntifkey="29" /label=Pbad\promoter RBS 3095..3106 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency RBS 139..150 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency terminator 5239..5318 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 2820..2899 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 5327..5367 /vntifkey="43" /label=TE\from\coliphageT7 terminator 2908..2948 /vntifkey="43" /label=TE\from\coliphageT7 promoter 2957..3086 /vntifkey="29" /label=Pbad\promoter misc_feature 3113..5230 /vntifkey="21" /label=T7\DNA\polymerase BASE COUNT 1911 a 1797 c 1880 g 1849 t ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga acacgattaa catcgctaag 181 aacgacttct ctgacatcga actggctgct atcccgttca acactctggc tgaccattac 241 ggtgagcgtt tagctcgcga acagttggcc cttgagcatg agtcttacga gatgggtgaa 301 gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct 361 gccgccaagc ctctcatcac taccctactc cctaagatga ttgcacgcat caacgactgg 421 tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt cctgcaagaa 481 atcaagccgg aagccgtagc gtacatcacc attaagacca ctctggcttg cctaaccagt 541 gctgacaata caaccgttca ggctgtagca agcgcaatcg gtcgggccat tgaggacgag 601 gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa 661 caactcaaca agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct 721 gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca taaggaagac 781 tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg aatggttagc 841 ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga actcgcacct 901 gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc 961 caaccttgcg tagttcctcc taagccgtgg actggcatta ctggtggtgg ctattgggct 1021 aacggtcgtc gtcctctggc gctggtgcgt actcacagta agaaagcact gatgcgctac 1081 gaagacgttt acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg 1141 aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg 1201 gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga agacatcgac 1261 atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac 1321 aaggctcgca agtctcgccg tatcagcctt gagttcatgc ttgagcaagc caataagttt 1381 gctaaccata aggccatctg gttcccttac aacatggact ggcgcggtcg tgtttacgct 1441 gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag gactgcttac gctggcgaaa 1501 ggtaaaccaa tcggtaagga aggttactac tggctgaaaa tccacggtgc aaactgtgcg 1561 ggtgtcgata aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac 1621 atcatggctt gcgctaagtc tccactggag aacacttggt gggctgagca agattctccg 1681 ttctgcttcc ttgcgttctg ctttgagtac gctggggtac agcaccacgg cctgagctat 1741 aactgctccc ttccgctggc gtttgacggg tcttgctctg gcatccagca cttctccgcg 1801 atgctccgag atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga aaccgttcag 1861 gacatctacg ggattgttgc taagaaagtc aacgagattc tacaagcaga cgcaatcaat 1921 gggaccgata acgaagtagt taccgtgacc gatgagaaca ctggtgaaat ctctgagaaa 1981 gtcaagctgg gcactaaggc actggctggt caatggctgg cttacggtgt tactcgcagt 2041 gtgactaagc gttcagtcat gacgctggct tacgggtcca aagagttcgg cttccgtcaa 2101 caagtgctgg aagataccat tcagccagct attgattccg gcaagggtct gatgttcact 2161 cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt gagcgtgacg 2221 gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct gctggctgct 2281 gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt gcattgggta 2341 actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac gcgcttgaac 2401 ctgatgttcc tcggtcagtt ccgcttacag cctaccatta acaccaacaa agatagcgag 2461 attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag ccaagacggt 2521 agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga atcttttgca 2581 ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt caaagcagtg 2641 cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt ctacgaccag 2701 ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc ggctaaaggt 2761 aacttgaacc tccgtgacat cttagagtcg gacttcgcgt tcgcgtaata atactagagc 2821 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 2881 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 2941 cgtttatata ctagagacat tgattatttg cacggcgtca cactttgcta tgccatagca 3001 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3061 tctccatacc gtttttttgg gctagctact agagaaagag gagaaatact agatgatcgt 3121 ttctgacatc gaagctaacg ccctcttaga gagcgtcact aagttccact gcggggttat 3181 ctacgactac tccaccgctg agtacgtaag ctaccgtccg agtgacttcg gtgcgtatct 3241 ggatgcgctg gaagccgagg ttgcacgagg cggtcttatt gtgttccaca acggtcacaa 3301 gtatgacgtt cctgcattga ccaaactggc aaagttgcaa ttgaaccgag agttccacct 3361 tcctcgtgag aactgtattg acacccttgt gttgtcacgt ttgattcatt ccaacctcaa 3421 ggacaccgat atgggtcttc tgcgttccgg caagttgccc ggaaaacgct ttgggtctca 3481 cgctttggag gcgtggggtt atcgcttagg cgagatgaag ggtgaataca aagacgactt 3541 taagcgtatg cttgaagagc agggtgaaga atacgttgac ggaatggagt ggtggaactt 3601 caacgaagag atgatggact ataacgttca ggacgttgtg gtaactaaag ctctccttga 3661 gaagctactc tctgacaaac attacttccc tcctgagatt gactttacgg acgtaggata 3721 cactacgttc tggtcagaat cccttgaggc cgttgacatt gaacatcgtg ctgcatggct 3781 gctcgctaaa caagagcgca acgggttccc gtttgacaca aaagcaatcg aagagttgta 3841 cgtagagtta gctgctcgcc gctctgagtt gctccgtaaa ttgaccgaaa cgttcggctc 3901 gtggtatcag cctaaaggtg gcactgagat gttctgccat ccgcgaacag gtaagccact 3961 acctaaatac cctcgcatta agacacctaa agttggtggt atctttaaga agcctaagaa 4021 caaggcacag cgagaaggcc gtgagccttg cgaacttgat acccgcgagt acgttgctgg 4081 tgctccttac accccagttg aacatgttgt gtttaaccct tcgtctcgtg accacattca 4141 gaagaaactc caagaggctg ggtgggtccc gaccaagtac accgataagg gtgctcctgt 4201 ggtggacgat gaggtactcg aaggagtacg tgtagatgac cctgagaagc aagccgctat 4261 cgacctcatt aaagagtact tgatgattca gaagcgaatc ggacagtctg ctgagggaga 4321 caaagcatgg cttcgttatg ttgctgagga tggtaagatt catggttctg ttaaccctaa 4381 tggagcagtt acgggtcgtg cgacccatgc gttcccaaac cttgcgcaaa ttccgggtgt 4441 acgttctcct tatggagagc agtgtcgcgc tgcttttggc gctgagcacc atttggatgg 4501 gataactggt aagccttggg ttcaggctgg catcgacgca tccggtcttg agctacgctg 4561 cttggctcac ttcatggctc gctttgataa cggcgagtac gctcacgaga ttcttaacgg 4621 cgacatccac actaagaacc agatagctgc tgaactacct acccgagata acgctaagac 4681 gttcatctat gggttcctct atggtgctgg tgatgagaag attggacaga ttgttggtgc 4741 tggtaaagag cgcggtaagg aactcaagaa gaaattcctt gagaacaccc ccgcgattgc 4801 agcactccgc gagtctatcc aacagacact tgtcgagtcc tctcaatggg tagctggtga 4861 gcaacaagtc aagtggaaac gccgctggat taaaggtctg gatggtcgta aggtacacgt 4921 tcgtagtcct cacgctgcct tgaataccct actgcaatct gctggtgctc tcatctgcaa 4981 actgtggatt atcaagaccg aagagatgct cgtagagaaa ggcttgaagc atggctggga 5041 tggggacttt gcgtacatgg catgggtaca tgatgaaatc caagtaggct gccgtaccga 5101 agagattgct caggtggtca ttgagaccgc acaagaagcg atgcgctggg ttggagacca 5161 ctggaacttc cggtgtcttc tggataccga aggtaagatg ggtcctaatt gggcgatttg 5221 ccactgataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 5281 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 5341 cttcgggtgg gcctttctgc gtttatatac tagtagcggc cgctgcagtc cggcaaaaaa 5401 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat 5461 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 5521 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 5581 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 5641 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 5701 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 5761 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 5821 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 5881 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 5941 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6001 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6061 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 6121 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 6181 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 6241 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 6301 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 6361 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc 6421 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt 6481 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct 6541 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac 6601 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat 6661 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa 6721 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt 6781 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa 6841 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat 6901 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg 6961 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat 7021 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg 7081 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc 7141 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag 7201 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt 7261 gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc 7321 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa 7381 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagag //