LOCUS pSB1C3 2948 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(10 #3# 0) 1 2 0 0 Nil -386 100) COMMENT (CObjectList COMMENT #219=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #132# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 4.40059 1.9 COMMENT 0.082322 1) COMMENT #220=(CLabel (CWidget 0 (0 0) 1 2 0 0 #134# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Ahl}I (881)" "@N (@S)" 5 13.645 COMMENT -5.22699 0 -10 3.27191 0.799245 #219#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #221=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -456 100) COMMENT (CObjectList) (CObjectList)) COMMENT #222=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1293 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 879..899 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 900..971 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 908..942 /vntifkey="39" /label=Stem\loop primer_bind complement(1035..1054) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1139..1753) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1154..1154 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1920..2025 /vntifkey="39" /label=T0\terminator CDS complement(2038..2697) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2811..2830 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2887..2890) /vntifkey="39" /label=end\of\terminator stem_loop complement(2900..2919) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..878 /vntifkey="21" /label=BBa_E0420 RBS 1..12 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency misc_feature 19..741 /vntifkey="21" /label=engineered\cyan\fluorescent\protein\derived\from\A.\victoria\GFP terminator 750..829 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 838..878 /vntifkey="43" /label=TE\from\coliphage\T7 BASE COUNT 783 a 782 c 719 g 664 t ORIGIN 1 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 61 atcctggtcg agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc 121 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 181 cccgtgccct ggcccaccct cgtgaccacc ctgacctggg gcgtgcagtg cttcagccgc 241 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 421 ggcaacatcc tggggcacaa gctggagtac aactacatca gccacaacgt ctatatcacc 481 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac 541 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 601 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 661 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 721 gacgagctgt acaagtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc 781 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 841 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagtagcgg ccgctgcagt 901 ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta 961 cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 1021 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 1081 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 1141 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 1201 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 1261 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 1321 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 1381 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 1441 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 1501 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 1561 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg 1621 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 1681 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 1741 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 1801 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 1861 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt 1921 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg 1981 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta 2041 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg 2101 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc 2161 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac 2221 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc 2281 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata 2341 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc 2401 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc 2461 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat 2521 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc 2581 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc 2641 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt 2701 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat 2761 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg 2821 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga 2881 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 2941 ttctagag //