LOCUS pSB1C3 3139 bp DNA circular 20-AUG-2017 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|-11904098| COMMENT VNTNAME|pSB1C3| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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0) 1 2 0 0 Nil -707 100) COMMENT (CObjectList) (CObjectList)) COMMENT #258=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -707 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 92 52970728 (CObList))) FEATURES Location/Qualifiers misc_feature 1070..1090 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 1091..1162 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 1099..1133 /vntifkey="39" /label=Stem\loop primer_bind complement(1226..1245) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1330..1944) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1345..1345 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 2111..2216 /vntifkey="39" /label=T0\terminator CDS complement(2229..2888) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 3002..3021 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(3078..3081) /vntifkey="39" /label=end\of\terminator stem_loop complement(3091..3110) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix promoter 1..200 /vntifkey="30" /label=promoter\(lacI\regulated) RBS 209..220 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency CDS 227..932 /vntifkey="4" /label=**highly**\engineered\mutant\of\red\fluorescent\protein\from\Discosoma\striata\(coral) terminator 941..1020 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 1029..1069 /vntifkey="43" /label=TE\from\coliphageT7 BASE COUNT 856 a 774 c 740 g 769 t ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga 241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca 301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa 361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt 421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact 481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt 541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg 601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc 661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct 721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa 781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa 841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc 901 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga 961 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1021 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagtagcg 1081 gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac 1141 cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg 1201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 1261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 1321 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa 1381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 1441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 1501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 1561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 1621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 1681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 1741 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 1801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 1861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 1921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 1981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 2041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 2101 gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca 2161 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg 2221 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt 2281 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc 2341 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc 2401 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa 2461 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca 2521 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat 2581 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc 2641 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca 2701 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag 2761 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc 2821 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt 2881 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt 2941 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt 3001 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 3061 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa 3121 ttcgcggccg cttctagag //