LOCUS pSB1C3 2784 bp DNA circular 19-MAR-1980 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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0 Nil -349 100) COMMENT (CObjectList) (CObjectList)) COMMENT #223=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -200 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 10 COMMENT (CStringList) 1888921824 0 (CObList))) FEATURES Location/Qualifiers misc_feature 715..735 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 736..807 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 744..778 /vntifkey="39" /label=Stem\loop primer_bind complement(871..890) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(975..1589) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 990..990 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1756..1861 /vntifkey="39" /label=T0\terminator CDS complement(1874..2533) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2647..2666 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2723..2726) /vntifkey="39" /label=end\of\terminator stem_loop complement(2736..2755) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..714 /vntifkey="21" /label=BBa_K1033910 BASE COUNT 754 a 674 c 671 g 685 t ORIGIN 1 atgacggcac tgactgaagg cgcaaaactg ttcgagaaag aaatcccata tatcactgag 61 ctggaaggtg acgttgaagg tatgaagttt atcatcaagg gtgaaggtac cggtgacgcg 121 agcgtcggta aagtggatgc tcagttcatt tgtaccacgg gcgacgttcc ggttccgtgg 181 agcacgctgg tcaccacgct gacgtatggt gctcagtgct ttgccaagta tccgcgccac 241 attgcggatt tcttcaaaag ctgcatgccg gaaggttacg tccaagagcg caccatcacc 301 tttgagggtg atggcgtgtt caagacccgt gcggaagtca cctttgaaaa tggcagcgtg 361 tacaaccgtg taaaactgaa cggccagggt ttcaagaagg acggccacgt gctgggcaaa 421 aatctggagt ttaactttac ccctcattgt ttgtacattt ggggtgacca agcgaatcat 481 ggcctgaaga gcgcgttcaa aatcatgcat gagatcaccg gctccaaaga ggatttcatt 541 gttgccgatc acacccaaat gaataccccg attggtggtg gtccggtgca cgtgccggag 601 taccaccaca ttacgtatca tgttaccctg tctaaagacg tcaccgatca ccgtgaccat 661 ttgaacattg ttgaggtgat caaggcagtt gacctggaga cgtaccgtta ataatactag 721 tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt 781 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc 841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 961 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca 1021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 1081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 1141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 1201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 1261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 1321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 1381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 1441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 1501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 1561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 1621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 1681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 1741 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct 1801 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga 1861 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa 1921 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca 1981 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt 2041 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga 2101 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg 2161 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga 2221 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc 2281 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc 2341 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta 2401 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa 2461 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga 2521 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc 2581 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact 2641 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg 2701 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc 2761 tggaattcgc ggccgcttct agag //