LOCUS 14D-5-\BBa 2773 bp DNA circular 14-SEP-2017 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-11940098| COMMENT VNTDBDATE|-11940098| COMMENT VNTNAME|14D-5- BBa_K1429001| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 10 COMMENT (CStringList) 1888921824 0 (CObList))) FEATURES Location/Qualifiers misc_feature 704..724 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 725..796 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 733..767 /vntifkey="39" /label=Stem\loop primer_bind complement(860..879) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(964..1578) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 979..979 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1745..1850 /vntifkey="39" /label=T0\terminator CDS complement(1863..2522) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2636..2655 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2712..2715) /vntifkey="39" /label=end\of\terminator stem_loop complement(2725..2744) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..703 /vntifkey="21" /label=BBa_K1429001 BASE COUNT 752 a 675 c 668 g 678 t ORIGIN 1 cgagacctta ggaggtaaac atatgtccct gtcgaaacaa gtactgccac acgatgttaa 61 gatgcgctat catatggatg gctgcgttaa tggtcattct ttcaccattg agggtgaagg 121 tgcaggcaaa ccgtatgagg gcaagaagat cttggaactg cgcgtgacga aaggtggccc 181 gctgcctttt gcgttcgata tcctgagcag cgtttttacc tacggtaacc gttgtttttg 241 cgagtatcca gaggacatgc cggactactt taaacagagc ctgccggaag gtcattcttg 301 ggaacgcacc ctgatgtttg aggatggcgg ttgtggtacg gcgagcgcgc acatttccct 361 ggacaagaac tgcttcgtgc acaagagcac cttccacggc gtcaatttcc cggcaaacgg 421 tccggtcatg caaaagaaag ctatgaactg ggagccgagc agcgaactga ttacggcgtg 481 cgacggtatc ctgaaaggcg atgtgaccat gtttctgttg ctggaaggtg gccaccgtct 541 taaatgtcag ttcaccacca gctacaaagc ccacaaggca gttaagatgc cgccgaatca 601 cattatcgaa cacgtgcttg ttaaaaaaga ggttgccgac ggctttcaga tccaagagca 661 tgcggtcgca aagcacttca ccgtcgacgt taaagaaacg taatactagt agcggccgct 721 gcagtccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa 781 gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 961 ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac 1021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 1081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 1141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 1201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 1261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 1321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 1381 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 1441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 1501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 1561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 1621 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 1681 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga 1741 ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca 1801 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca 1861 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg 1921 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg 1981 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg 2041 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata 2101 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc 2161 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac 2221 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc 2281 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg 2341 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta 2401 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa 2461 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc 2521 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat 2581 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc 2641 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 2701 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg 2761 gccgcttcta gag //