LOCUS pSB1C3 2794 bp DNA circular 19-MAR-1980 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#223=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -200 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 10 COMMENT (CStringList) 1888921824 0 (CObList))) FEATURES Location/Qualifiers misc_feature 725..745 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 746..817 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 754..788 /vntifkey="39" /label=Stem\loop primer_bind complement(881..900) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(985..1599) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1000..1000 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1766..1871 /vntifkey="39" /label=T0\terminator CDS complement(1884..2543) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2657..2676 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2733..2736) /vntifkey="39" /label=end\of\terminator stem_loop complement(2746..2765) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..724 /vntifkey="21" /label=BBa_K1429002 BASE COUNT 753 a 681 c 674 g 686 t ORIGIN 1 cgagacctta ggaggtaaac atatgtcgtc tggtgccaaa ttgtttacga agaagatccc 61 gtatgtcact gaactcgagg gcgacgtcaa tggtatgaag tttaccattc atggtaaagg 121 taccggcgat gcgagcacgg gtaccattga tgcgcagttc atctgcacta cgggcgacct 181 gccggtcccg tgggcaaccc tggtgagcac cctgagctac ggtgttcagt gtttcgccaa 241 gtacccgccg gagctgaagg atttctttaa gagctgcatg ccggaaggtt atgttcaaga 301 gcgtaccatc accttcgaag gcgacggcgt gtttaagacg cgtgctgagg ttacctttga 361 aaacggttct gtctacaatc gtgtcaaact gaacggccag ggttttaaga aagacggtca 421 cattctgcgt aagaacgttg cattccaatg cccgccagat attgtgtata ttctgcctga 481 caccgttaac aatggcatcc gcgttgagtt caaccaggcg tacgatattg aaggtgtgac 541 cgaaaaactg gttaccaaat gcagccaaat gaatcgtccg ttggcgggct ccgcggcagt 601 gcatatcccg cgttatcatc acctgagcaa acacaccaaa ctgagcaaag accgcgacga 661 gcgccgtgat cacatgtgtc tggtagaggt cgtgaaagcg gttgatctgg acacgtatca 721 gtaatactag tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc 781 ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact 841 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 901 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 961 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg 1021 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 1081 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 1141 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 1201 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 1261 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 1321 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 1381 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 1441 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 1501 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 1561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 1621 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 1681 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 1741 aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac 1801 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga 1861 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt 1921 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc 1981 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg 2041 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga 2101 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca 2161 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat 2221 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga 2281 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca 2341 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt 2401 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca 2461 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta 2521 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca 2581 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc 2641 ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 2701 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa 2761 ggatgatttc tggaattcgc ggccgcttct agag //