LOCUS pSB1C3 3131 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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misc_feature 1062..1082 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 1083..1154 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 1091..1125 /vntifkey="39" /label=Stem\loop primer_bind complement(1218..1237) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1322..1936) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1337..1337 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 2103..2208 /vntifkey="39" /label=T0\terminator CDS complement(2221..2880) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2994..3013 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(3070..3073) /vntifkey="39" /label=end\of\terminator stem_loop complement(3083..3102) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..1061 /vntifkey="21" /label=BBa_K316003 misc_feature 1..958 /vntifkey="21" /label=xylE\reporter\gene\with\rbs terminator 967..1007 /vntifkey="43" /label=TE\from\coliphage\T7 BASE COUNT 805 a 798 c 773 g 755 t ORIGIN 1 ctcatgaact atgaagaggt gacgtcatga acaaaggtgt aatgcgaccg ggccatgtgc 61 agctgcgtgt actggacatg agcaaggccc tggaacacta cgtcgagttg ctgggcctga 121 tcgagatgga ccgtgacgac cagggccgtg tctatctgaa ggcttggacc gaagtggata 181 agttttccct ggtgctacgc gaggctgacg agccgggcat ggattttatg ggtttcaagg 241 ttgtggatga ggatgctctc cggcaactgg agcgggatct gatggcatat ggctgtgccg 301 ttgagcagct acccgcaggt gaactgaaca gttgtggccg gcgcgtgcgc ttccaggccc 361 cctccgggca tcacttcgag ttgtatgcag acaaggaata tactggaaag tggggtttga 421 atgacgtcaa tcccgaggca tggccgcgcg atctgaaagg tatggcggct gtgcgtttcg 481 accacgccct catgtatggc gacgaattgc cggcgaccta tgacctgttc accaaggtgc 541 tcggtttcta tctggccgaa caggtgctgg acgaaaatgg cacgcgcgtc gcccagtttc 601 tcagtctgtc gaccaaggcc cacgacgtgg ccttcattca ccatccggaa aaaggccgcc 661 tccatcatgt gtccttccac ctcgaaacct gggaagactt gcttcgcgcc gccgacctga 721 tctccatgac cgacacatct atcgatatcg gcccaacccg ccacggcctc actcacggca 781 agaccatcta cttcttcgac ccgtccggta accgcaacga agtgttctgc gggggagatt 841 acaactaccc ggaccacaaa ccggtgacct ggaccaccga ccagctgggc aaggcgatct 901 tttaccacga ccgcattctc aacgaacgat tcatgaccgt gctgacctga tggtccggta 961 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagaga 1021 atataaaaag ccagattatt aatccggctt ttttattatt ttactagtag cggccgctgc 1081 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga 1141 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 1201 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 1261 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 1321 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc 1381 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 1441 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 1501 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 1561 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 1621 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 1681 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 1741 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 1801 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 1861 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 1921 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 1981 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 2041 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg 2101 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga 2161 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa 2221 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac 2281 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc 2341 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc 2401 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt 2461 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga 2521 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt 2581 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc 2641 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg 2701 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat 2761 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg 2821 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat 2881 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct 2941 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga gtgccacctg 3001 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 3061 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc 3121 cgcttctaga g //