LOCUS pSB1C3 4696 bp DNA circular 20-AUG-2017 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|-11904098| COMMENT VNTNAME|pSB1C3| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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"{\\i Ava}I (3660)" "@N (@S)" 5 -14.3388 COMMENT 1.74233 0 -10 3.97125 0.799245 #234#) COMMENT #236=(CScratch COMMENT (CWidget 2 (1 #1# 2) 1 2 0 0 #143# 268633231 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.193699 1.9 COMMENT 0.082322 1) COMMENT #237=(CLabel (CWidget 0 (0 0) 1 2 0 0 #135# 1 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (4552)" "@N (@S)" 5 -8.92148 COMMENT 10.454 0 -10 3.79641 0.799245 #236#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #238=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -456 100) COMMENT (CObjectList) (CObjectList)) COMMENT #239=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -835 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 148632758 COMMENT (CStringList) 148632754 2147483648 (CObList))) FEATURES Location/Qualifiers misc_feature 2627..2647 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 2648..2719 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 2656..2690 /vntifkey="39" /label=Stem\loop primer_bind complement(2783..2802) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(2887..3501) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 2902..2902 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 3668..3773 /vntifkey="39" /label=T0\terminator CDS complement(3786..4445) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 4559..4578 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(4635..4638) /vntifkey="39" /label=end\of\terminator stem_loop complement(4648..4667) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix CDS 955..2626 /vntifkey="4" /label=EPIC\Firefly\Luciferase BASE COUNT 1201 a 1122 c 1235 g 1138 t ORIGIN 1 ttaaggaggt aaaaaaaatg ggtccggtcg ttgaaaagat cgccgagctg ggtaagtaca 61 cggttggtga gggtccgcac tgggaccacg aaacgcagac gctgtatttc gtggatacgg 121 tcgagaaaac gttccacaaa tacgttccga gccagaagaa atacacgttt tgtaaggtgg 181 acaagctggt tagctttatc attccgctgg caggttctcc gggtcgcttc gtcgtcagct 241 tggagcgcga gattgcgatc ctgacgtggg acggcgtcag cgcggcaccg accagcattg 301 aggcgattgt gaatgtcgag ccgcacatca agaataatcg cctgaacgac ggtaaagcgg 361 acccgctggg taatctgtgg accggcacga tggccattga tgcgggtttg ccggtgggtc 421 cggtgaccgg ctccctgtac cacctgggtg cggataagaa ggtcaagatg catgaaagca 481 atattgccat cgctaacggc ctggcgtgga gcaacgacct gaagaagatg tactatattg 541 acagcggcaa acgtcgtgtt gacgaatacg actatgatgc gagcacgctg tctatcagca 601 atcagcgtcc gctgttcacc ttcgaaaaac atgaggtccc tggctaccca gacggtcaaa 661 ccattgacga agaaggtaac ctgtgggtcg ccgtgtttca aggccagcgt attatcaaga 721 ttagcaccca gcagccggag gttctgctgg ataccgtcaa aatcccggac ccgcaggtga 781 ctagcgttgc attcggtggt ccgaatctgg acgagctgca tgtgaccagc gccggtttgc 841 agctggatga cagctccctg gacaaatctt tggtcaatgg tcacgtctac cgtgtgactg 901 gcctgggtgt taagggtttc gccggtgtga aagttaagct gtaataagga tccttaagga 961 ggtaaaaaaa atggaggacg cgaaaaacat caagaaaggc ccagcaccgt tctatccgct 1021 ggaagatggt accgccggtg aacaattgca caaagcgatg aaacgttatg cgctggttcc 1081 gggtaccatc gcattcacgg acgcgcatat cgaggttaac atcacctatg ccgagtactt 1141 cgagatgagc gttcgtctgg ccgaggcaat gaagcgttat ggtttgaata ccaatcatcg 1201 catcgtcgtc tgtagcgaga acagcttgca gtttttcatg ccagttctgg gtgcactgtt 1261 tattggtgtg gcagtggcgc ctgcgaatga tatctacaac gagcgtgaac tgttgaatag 1321 catgaacatt tcgcagccga cggttgtttt tgtgtccaag aaaggtttgc agaagattct 1381 gaatgtgcaa aagaagttgc cgatcattca gaaaatcatc atcatggaca gcaagaccga 1441 ttaccagggt ttccaaagca tgtacacttt tgtgacgagc cacctgcctc cgggttttaa 1501 cgagtatgat ttcgttccgg aaagcttcga ccgtgataag acgattgcac tgattatgaa 1561 ctcgtccggc tctaccggcc tgcctaaagg cgtggccctg ccgcaccgta ctgcatgtgt 1621 ccgcttcagc cacgcgcgtg atccgatctt cggtaaccaa atcattccgg acaccgccat 1681 tttgtctgtc gttccgtttc atcatggctt tggtatgttt accactctgg gttacctgat 1741 ctgtggtttc cgtgttgttc tgatgtaccg tttcgaagaa gaactgttcc tgcgtagcct 1801 gcaagattac aagatccaat ccgcgctgct ggtgccgacg ctgtttagct tcttcgctaa 1861 atccaccctg attgacaaat atgacttgag caacctgcac gaaatcgcgt cgggcggtgc 1921 gccgctgagc aaagaagtcg gtgaggccgt tgcgaaacgt ttccatctgc cgggcatccg 1981 tcagggttat ggcctgacgg aaacgaccag cgcgattttg attacccctg agggtgacga 2041 taaaccgggt gccgttggca aggtggtacc gttctttgag gctaaagtcg tggacctgga 2101 cacgggtaag actctgggtg tgaatcaacg tggcgagttg tgcgttcgtg gcccgatgat 2161 catgagcggt tatgtcaaca acccggaagc aactaatgcg ttgatcgata aggatggctg 2221 gctgcacagc ggcgacctgg cgtactggga tgaggatgaa cactttttca tcgttggtcg 2281 cctgaagagc ctgatcaagt acaagggcta ccaggtggca ccggctgaac tggagagcat 2341 tctgttgcaa caccctaata tcttcgacgc gggtgttgcg ggtctgcctg atgatgatgc 2401 cggtgaattg ccagctgcgg tggtcgttct ggaacacggt aaaacgatga ctgaaaaaga 2461 aatcgtggac tacgtcgcct cgcaggtgac caccgctaaa aagttgcgtg gtggcgtggt 2521 gtttgtggat gaagtgccga aaggcctgac gggtaaacgt gatgcacgta agattcgtga 2581 gatcttgatc aaggcgaaga aaggtggtaa atccaagttg taataatact agtagcggcc 2641 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga 2701 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc 2761 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 2821 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 2881 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc 2941 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 3001 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 3061 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 3121 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 3181 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 3241 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 3301 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 3361 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 3421 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 3481 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 3541 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 3601 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct 3661 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat 3721 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata 3781 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg 3841 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc 3901 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata 3961 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac 4021 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct 4081 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa 4141 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc 4201 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga 4261 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc 4321 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca 4381 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc 4441 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt 4501 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc 4561 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 4621 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc 4681 gcggccgctt ctagag //