LOCUS pSB1C3 4961 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList))) (CObjectList)) COMMENT #200=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -874 100) COMMENT (CObjectList) (CObjectList)) COMMENT #201=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -874 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 1 (CObList))) FEATURES Location/Qualifiers misc_feature 2892..2912 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 2913..2984 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 2921..2955 /vntifkey="39" /label=Stem\loop primer_bind complement(3048..3067) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(3152..3766) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 3167..3167 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 3933..4038 /vntifkey="39" /label=T0\terminator CDS complement(4051..4710) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 4824..4843 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(4900..4903) /vntifkey="39" /label=end\of\terminator stem_loop complement(4913..4932) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..2891 /vntifkey="21" /label=BBa_K325108 misc_feature 1219..2891 /vntifkey="21" /label=EPIC\Firefly\Luciferase\Photinus\Pyralis\(E.\coli\optimised) BASE COUNT 1286 a 1228 c 1224 g 1223 t ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtt aaggaggtaa aaaaaatgga ggacgcgaaa aacatcaaga 1261 aaggcccagc accgttctat ccgctggaag atggtaccgc cggtgaacaa ttgcacaaag 1321 cgatgaaacg ttatgcgctg gttccgggta ccatcgcatt cacggacgcg catatcgagg 1381 ttaacatcac ctatgccgag tacttcgaga tgagcgttcg tctggccgag gcaatgaagc 1441 gttatggttt gaataccaat catcgcatcg tcgtctgtag cgagaacagc ttgcagtttt 1501 tcatgccagt tctgggtgca ctgtttattg gtgtggcagt ggcgcctgcg aatgatatct 1561 acaacgagcg tgaactgttg aatagcatga acatttcgca gccgacggtt gtttttgtgt 1621 ccaagaaagg tttgcagaag attctgaatg tgcaaaagaa gttgccgatc attcagaaaa 1681 tcatcatcat ggacagcaag accgattacc agggtttcca aagcatgtac acttttgtga 1741 cgagccacct gcctccgggt tttaacgagt atgatttcgt tccggaaagc ttcgaccgtg 1801 ataagacgat tgcactgatt atgaactcgt ccggctctac cggcctgcct aaaggcgtgg 1861 ccctgccgca ccgtactgca tgtgtccgct tcagccacgc gcgtgatccg atcttcggta 1921 accaaatcat tccggacacc gccattttgt ctgtcgttcc gtttcatcat ggctttggta 1981 tgtttaccac tctgggttac ctgatctgtg gtttccgtgt tgttctgatg taccgtttcg 2041 aagaagaact gttcctgcgt agcctgcaag attacaagat ccaatccgcg ctgctggtgc 2101 cgacgctgtt tagcttcttc gctaaatcca ccctgattga caaatatgac ttgagcaacc 2161 tgcacgaaat cgcgtcgggc ggtgcgccgc tgagcaaaga agtcggtgag gccgttgcga 2221 aacgtttcca tctgccgggc atccgtcagg gttatggcct gacggaaacg accagcgcga 2281 ttttgattac ccctgagggt gacgataaac cgggtgccgt tggcaaggtg gtaccgttct 2341 ttgaggctaa agtcgtggac ctggacacgg gtaagactct gggtgtgaat caacgtggcg 2401 agttgtgcgt tcgtggcccg atgatcatga gcggttatgt caacaacccg gaagcaacta 2461 atgcgttgat cgataaggat ggctggctgc acagcggcga cctggcgtac tgggatgagg 2521 atgaacactt tttcatcgtt ggtcgcctga agagcctgat caagtacaag ggctaccagg 2581 tggcaccggc tgaactggag agcattctgt tgcaacaccc taatatcttc gacgcgggtg 2641 ttgcgggtct gcctgatgat gatgccggtg aattgccagc tgcggtggtc gttctggaac 2701 acggtaaaac gatgactgaa aaagaaatcg tggactacgt cgcctcgcag gtgaccaccg 2761 ctaaaaagtt gcgtggtggc gtggtgtttg tggatgaagt gccgaaaggc ctgacgggta 2821 aacgtgatgc acgtaagatt cgtgagatct tgatcaaggc gaagaaaggt ggtaaatcca 2881 agttgtaata atactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca 2941 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc 3001 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 3061 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 3121 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc 3181 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 3241 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 3301 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 3361 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 3421 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 3481 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 3541 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 3601 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 3661 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 3721 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 3781 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 3841 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 3901 tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg 3961 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc 4021 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta 4081 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac 4141 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa 4201 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac 4261 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag 4321 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc 4381 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca 4441 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg 4501 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt 4561 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca 4621 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac 4681 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga 4741 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct 4801 tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat 4861 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt 4921 tcgctaagga tgatttctgg aattcgcggc cgcttctaga g //