LOCUS pSB1C3 4955 bp DNA circular 20-AUG-2017 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|-11904098| COMMENT VNTNAME|pSB1C3| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 4955 COMMENT (CDBMol 0 0 1 1 1 0 0 53643184 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 40 50 0 1 0 COMMENT (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" COMMENT "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 4955 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 3 1585 0 2085 0 3413 0 0 COMMENT "") COMMENT #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 4 60 0 1836 0 3919 0 4811 0 COMMENT 0 "") #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1145 0 0 "") COMMENT #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") COMMENT #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4935 0 0 "") COMMENT #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") COMMENT #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 1974 0 4186 0 0 "") COMMENT #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 2906 0 0 "") COMMENT #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") COMMENT #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) COMMENT (CObList COMMENT #10=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 2886 2906 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #11=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 2907 2978 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #12=(CFSignal (CObList) "Stem loop" 39 0 0 2915 2949 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #13=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 3042 3061 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #14=(CFSignal (CObList) "rep (pMB1)" 21 0 1 3146 3760 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #15=(CFSignal (CObList) "ORI" 21 0 0 3161 3161 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #16=(CFSignal (CObList) "T0 terminator" 39 0 0 3927 4032 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #17=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 4045 4704 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #18=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 4818 4837 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #19=(CFSignal (CObList) "end of terminator" 39 0 1 4894 4897 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #20=(CFSignal (CObList) "Stem loop" 39 0 1 4907 4926 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #21=(CFSignal (CObList) "mutated version of Luciferase/LRE" 4 0 0 1219 COMMENT 2885 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #22=(CFSignal (CObList) "Inducible pBad/araC promoter" 30 0 0 1 1210 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #23=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #24=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #25=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. Author: Demo User. Original author: UNKNOWN" COMMENT 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Creation Date: ? (no data)" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modified: 08/20/17 03:53" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 4955 bp" 1) COMMENT #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #34=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Modification Date in the Original DB: 20-AUG-2017" 1) COMMENT #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Associated Genes: rep (pMB1); ORI; Chloramphenicol resistance" COMMENT 1))) "Standard Fields") COMMENT #38=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "Demo User" COMMENT 1))) "Author") COMMENT #40=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #41=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #42=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #43=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #44=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1219 (3 #21# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #45=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1219 End: 2885" 1))) COMMENT "mutated version of Luciferase/LRE") COMMENT #46=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4045 (3 #17# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #47=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4045 End: 4704 (Complementary)" COMMENT 1) COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #49=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1160..1819)" 1) COMMENT #50=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #51=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"Chloramphenicol resistance\"" COMMENT 1))) "Chloramphenicol resistance"))) COMMENT "CDS (2 total)") COMMENT #52=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #53=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2886 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2886 End: 2906" 1) COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1..21" 1))) "DNA: BioBrick suffix") COMMENT #57=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3146 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3146 End: 3760 (Complementary)" COMMENT 1) COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Unknown feature type:misc" 1) COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #61=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(261..875)" 1) COMMENT #62=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"rep (pMB1)\"" 1))) COMMENT "rep (pMB1)") COMMENT #64=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3161 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3161 End: 3161" 1) COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Unknown feature type:misc" 1) COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 276..276" 1) COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"ORI\"" 1))) "ORI"))) COMMENT "Misc. Feature (3 total)") COMMENT #71=(CFolderPar COMMENT (CGroupPar (CParagraph 28 (7 28 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #72=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3042 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3042 End: 3061 (Complementary)" COMMENT 1) COMMENT #74=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(157..176)" 1))) COMMENT "Verification reverse (VR) primer binding site") COMMENT #76=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4818 (3 #18# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4818 End: 4837" 1) COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1933..1952" 1))) COMMENT "Verification forward (VF2) primer binding site"))) COMMENT "Primer Binding Site (2 total)") COMMENT #80=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #81=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #22# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 1210" 1))) COMMENT "Inducible pBad/araC promoter"))) COMMENT "Promoter Prokaryotic (1 total)") COMMENT #83=(CFolderPar COMMENT (CGroupPar (CParagraph 39 (7 39 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #84=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2907 (3 #11# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #85=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2907 End: 2978" 1) COMMENT #86=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 22..93" 1))) COMMENT "E. coli his operon terminator") COMMENT #88=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2915 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #89=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2915 End: 2949" 1) COMMENT #90=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #91=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 30..64" 1))) "Stem loop") COMMENT #92=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3927 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #93=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3927 End: 4032" 1) COMMENT #94=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1042..1147" 1))) "T0 terminator") COMMENT #96=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4894 (3 #19# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #97=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4894 End: 4897 (Complementary)" COMMENT 1) COMMENT #98=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #99=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2009..2012)" 1))) COMMENT "end of terminator") COMMENT #100=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4907 (3 #20# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #101=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4907 End: 4926 (Complementary)" COMMENT 1) COMMENT #102=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #103=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2022..2041)" 1))) COMMENT "Stem loop"))) "Stem Loop (5 total)"))) COMMENT "Feature Map") COMMENT #104=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #105=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #106=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #107=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 0 End: 0 " 1) COMMENT #108=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #109=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 2051..2072" 1))) COMMENT "DNA: BioBrick prefix"))) COMMENT "Misc. Feature (1 total)"))) COMMENT "Imported Features Not Shown on Map") COMMENT #110=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #111=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 52915552 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #112=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #113=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 1585 " 1) COMMENT #114=(CLinePar COMMENT (CParagraph 0 (1 #0# 2) 1 2 2 0 191) COMMENT " N2: 2085 " 1) COMMENT #115=(CLinePar COMMENT (CParagraph 0 (1 #0# 3) 1 2 2 0 191) COMMENT " N3: 3413 " 1))))) "ApaLI: 3 sites") COMMENT 1 5 "GTGCAC" "CACGTG") COMMENT #116=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 52873944 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #117=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #118=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 60 " 1) COMMENT #119=(CLinePar COMMENT (CParagraph 0 (1 #1# 2) 1 2 2 0 191) COMMENT " N2: 1836 " 1) COMMENT #120=(CLinePar COMMENT (CParagraph 0 (1 #1# 3) 1 2 2 0 191) COMMENT " N3: 3919 " 1) COMMENT #121=(CLinePar COMMENT (CParagraph 0 (1 #1# 4) 1 2 2 0 191) COMMENT " N4: 4811 " 1))))) "AvaI: 4 sites") COMMENT 1 5 "CYCGRG" "GRGCYC") COMMENT #122=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 52917176 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #123=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #124=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 1145 " 1))))) "BamHI: 1 site") COMMENT 1 5 "GGATCC" "CCTAGG") COMMENT #125=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 52904384 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "ClaI: 0 sites") 2 4 "ATCGAT" COMMENT "TAGCTA") COMMENT #126=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 52904304 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #127=(CGroupPar COMMENT (CParagraph 0 (10 #4# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #128=(CLinePar COMMENT (CParagraph 0 (1 #4# 1) 1 2 2 0 191) COMMENT " N1: 4935 " 1))))) "EcoRI: 1 site") COMMENT 1 5 "GAATTC" "CTTAAG") COMMENT #129=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 52914112 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "HindIII: 0 sites") 1 5 "AAGCTT" COMMENT "TTCGAA") COMMENT #130=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 52914032 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #131=(CGroupPar COMMENT (CParagraph 0 (10 #6# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #132=(CLinePar COMMENT (CParagraph 0 (1 #6# 1) 1 2 2 0 191) COMMENT " N1: 1974 " 1) COMMENT #133=(CLinePar COMMENT (CParagraph 0 (1 #6# 2) 1 2 2 0 191) COMMENT " N2: 4186 " 1))))) "NcoI: 2 sites") COMMENT 1 5 "CCATGG" "GGTACC") COMMENT #134=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53367480 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #135=(CGroupPar COMMENT (CParagraph 0 (10 #7# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #136=(CLinePar COMMENT (CParagraph 0 (1 #7# 1) 1 2 2 0 191) COMMENT " N1: 2906 " 1))))) "PstI: 1 site") COMMENT 5 1 "CTGCAG" "GACGTC") COMMENT #137=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 52905768 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "SmaI: 0 sites") 3 3 "CCCGGG" COMMENT "GGGCCC") COMMENT #138=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 53353832 (8 0) 1 1 1 0 178) COMMENT (CObjectList)) "XmaI: 0 sites") 1 5 "CCCGGG" COMMENT "GGGCCC"))) "Restriction/Methylation Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #139=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #140=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #141=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #142=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #143=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #144=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #145=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #146=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #147=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #148=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #149=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #150=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #151=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #152=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #153=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #154=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #155=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #156=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #157=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #158=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #159=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #160=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #161=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #162=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #163=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #164=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #165=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #166=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #167=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #168=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #169=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #170=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #171=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #172=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #173=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #174=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #175=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #176=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #177=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #178=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 COMMENT -5842) 1 1 0 1 1 COMMENT (mapper: 30.8063 -19.587 40.0378 40.0378 0.01 10 -1 4955 4955 1 0 0) COMMENT #179=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 720906 100) COMMENT (CObjectList COMMENT #180=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1009 100) COMMENT (CObjectList COMMENT #181=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #145# 39681868 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27545 6.27345 1 0.0214037) 0.126603) COMMENT #182=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #142# 374 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "pSB1C3" "@N" 1 0 0.871165 0 -10 COMMENT 4.49575 1.39868 Nil) COMMENT #183=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #141# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "4955 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 2.59755 0.799245 Nil)) (CObjectList)) COMMENT #184=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -521 100) COMMENT (CObjectList COMMENT #185=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -730 100) COMMENT (CObjectList COMMENT #186=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #143# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 0.317774 1.15335 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #187=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #140# 268632818 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Chloramphenicol resistance" "@N" 1 -14.7563 COMMENT 8.71165 0 -10 12.2634 1.04901 #186#) COMMENT #188=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #21# 0) 1 2 0 0 #143# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 2.62068 4.73116 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #189=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #140# 268632636 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "mutated version of Luciferase/LRE" "@N" 1 COMMENT 14.3045 -12.1963 0 -10 15.0857 1.04901 #188#)) COMMENT (CObjectList)) COMMENT #190=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil -1218 100) COMMENT (CObjectList COMMENT #191=(CScratch COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #148# 39912726 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 2.62068 1.9 COMMENT 0.082322 1) COMMENT #192=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #140# 160218328 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "DNA: BioBrick suffix" "@N" 1 -11.2323 COMMENT -13.9386 0 -10 8.81667 1.04901 #191#) COMMENT #193=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #144# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 1.51291 2.29152 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #194=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #140# 160179908 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "rep (pMB1)" COMMENT "@N" 1 -14.2604 -1.74233 0 -10 4.77049 COMMENT 1.04901 #193#) COMMENT #195=(CScratch COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #148# 39963164 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 2.27253 1.9 COMMENT 0.082322 1) COMMENT #196=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #140# 268632496 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "ORI" "@N" 1 COMMENT -11.8019 -5.22699 0 -10 1.67342 1.04901 #195#)) COMMENT (CObjectList)) COMMENT #197=(CGroupWidget COMMENT (CWidget 28 (7 28 0) 1 2 0 0 Nil -102 100) COMMENT (CObjectList COMMENT #198=(CScratch COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #161# 54147568 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 2.42318 1.9 COMMENT 0.082322 1) COMMENT #199=(CLabel (CWidget 0 (0 0) 1 2 0 0 #140# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification reverse (VR) primer binding site" COMMENT "@N" 1 -19.4748 -6.96932 0 -10 19.1319 COMMENT 1.04901 #198#) COMMENT #200=(CScratch COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #161# 52986928 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.174712 1.9 COMMENT 0.082322 1) COMMENT #201=(CLabel (CWidget 0 (0 0) 1 2 0 0 #140# 84 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification forward (VF2) primer binding site" COMMENT "@N" 1 -12.5263 12.1963 0 -10 19.4816 1.04901 COMMENT #200#)) (CObjectList)) COMMENT #202=(CGroupWidget COMMENT (CWidget 30 (7 30 0) 1 2 0 0 Nil -177 100) COMMENT (CObjectList COMMENT #203=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #22# 0) 1 2 0 0 #156# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 COMMENT 4.74129 6.27319 1 0.082322) 0.8 1.8 0) COMMENT #204=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #140# 646 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Inducible pBad/araC promoter" "@N" 1 15.3536 COMMENT 8.71165 0 -10 12.9628 1.04901 #203#)) COMMENT (CObjectList)) COMMENT #205=(CGroupWidget COMMENT (CWidget 39 (7 39 0) 1 2 0 0 Nil -1288 100) COMMENT (CObjectList COMMENT #206=(CLine COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #161# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 0.835356 2.50294 COMMENT 2.5941) COMMENT #207=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #140# 160218608 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "E. coli his operon terminator" "@N" 1 COMMENT -14.8435 -8.71165 0 -10 12.3383 1.04901 #206#) COMMENT #208=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #161# 39969976 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 2.58397 1.9 COMMENT 0.082322 1) COMMENT #209=(CLabel (CWidget 0 (0 0) 1 2 0 0 #140# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Stem loop" "@N" COMMENT 1 -9.18118 -10.454 0 -10 4.42082 1.04901 COMMENT #208#) COMMENT #210=(CLine COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #161# COMMENT 1605442005 100) (LOGPEN 0 0 16711680) 1 COMMENT 0.835356 1.16855 1.30275) COMMENT #211=(CLabel (CWidget 0 (0 0) 1 2 0 0 #140# 30 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T0 terminator" COMMENT "@N" 1 -14.4995 3.48466 0 -10 5.74457 1.04901 COMMENT #210#) COMMENT #212=(CScratch COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #161# 39911953 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.0784939 1.9 COMMENT 0.082322 1) COMMENT #213=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #140# 160218956 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "end of terminator" "@N" 1 -5.60157 13.9386 0 COMMENT -10 7.41799 1.04901 #212#) COMMENT #214=(CScratch COMMENT (CWidget 0 (3 #20# 0) 1 2 0 0 #161# 39963376 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.0620355 1.9 COMMENT 0.082322 1) COMMENT #215=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #140# 268632216 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Stem loop" "@N" COMMENT 1 -3.93043 15.681 0 -10 4.42082 1.04901 #214#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #216=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -247 100) COMMENT (CObjectList COMMENT #217=(CGroupWidget COMMENT (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -1154 100) COMMENT (CObjectList COMMENT #218=(CScratch COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #147# 268751352 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 4.82485 1.9 COMMENT 0.082322 1) COMMENT #219=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #149# 268632356 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Bam}HI (1145)" "@N (@S)" 5 14.6694 COMMENT 1.74233 0 -10 4.49575 0.799245 #218#)) COMMENT (CObjectList)) COMMENT #220=(CGroupWidget COMMENT (CWidget 1 (10 #4# 0) 1 2 0 0 Nil -316 100) COMMENT (CObjectList COMMENT #221=(CScratch COMMENT (CWidget 1 (1 #4# 1) 1 2 0 0 #147# 39962520 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.0265867 1.9 COMMENT 0.082322 1) COMMENT #222=(CLabel (CWidget 0 (0 0) 1 2 0 0 #149# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Eco}RI (4935)" "@N (@S)" 5 -3.99605 COMMENT 17.4233 0 -10 4.32092 0.799245 #221#)) COMMENT (CObjectList)) COMMENT #223=(CGroupWidget COMMENT (CWidget 1 (10 #7# 0) 1 2 0 0 Nil -1014 100) COMMENT (CObjectList COMMENT #224=(CScratch COMMENT (CWidget 1 (1 #7# 1) 1 2 0 0 #147# 39963016 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 2.59536 1.9 COMMENT 0.082322 1) COMMENT #225=(CLabel (CWidget 0 (0 0) 1 2 0 0 #149# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Pst}I (2906)" "@N (@S)" 5 -9.24327 COMMENT -12.1963 0 -10 3.72148 0.799245 #224#)) COMMENT (CObjectList)) COMMENT #226=(CGroupWidget COMMENT (CWidget 2 (10 #6# 0) 1 2 0 0 Nil -1084 100) COMMENT (CObjectList COMMENT #227=(CScratch COMMENT (CWidget 1 (1 #6# 1) 1 2 0 0 #147# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 3.77531 1.9 COMMENT 0.082322 1) COMMENT #228=(CLabel (CWidget 0 (0 0) 1 2 0 0 #139# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Nco}I (1974)" "@N (@S)" 5 10.1357 COMMENT -10.454 0 -10 3.79641 0.799245 #227#) COMMENT #229=(CScratch COMMENT (CWidget 2 (1 #6# 2) 1 2 0 0 #147# 161907675 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.974844 1.9 COMMENT 0.082322 1) COMMENT #230=(CLabel (CWidget 0 (0 0) 1 2 0 0 #139# 75 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Nco}I (4186)" "@N (@S)" 5 -12.6256 COMMENT 6.96932 0 -10 3.87134 0.799245 #229#)) COMMENT (CObjectList)) COMMENT #231=(CGroupWidget COMMENT (CWidget 3 (10 #0# 0) 1 2 0 0 Nil -665 100) COMMENT (CObjectList COMMENT #232=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #147# 268751416 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 4.26779 1.9 COMMENT 0.082322 1) COMMENT #233=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #139# 160180172 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (1585)" "@N (@S)" 5 13.6767 COMMENT -5.22699 0 -10 4.24599 0.799245 #232#) COMMENT #234=(CScratch COMMENT (CWidget 2 (1 #0# 2) 1 2 0 0 #147# 39970484 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 3.63478 1.9 COMMENT 0.082322 1) COMMENT #235=(CLabel (CWidget 0 (0 0) 1 2 0 0 #139# 58 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (2085)" "@N (@S)" 5 9.27809 COMMENT -13.9386 0 -10 4.42082 0.799245 #234#) COMMENT #236=(CScratch COMMENT (CWidget 3 (1 #0# 3) 1 2 0 0 #147# 39961432 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 1.95349 1.9 COMMENT 0.082322 1) COMMENT #237=(CLabel (CWidget 0 (0 0) 1 2 0 0 #139# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Apa}LI (3413)" "@N (@S)" 5 -14.212 COMMENT -3.48466 0 -10 4.24599 0.799245 #236#)) COMMENT (CObjectList)) COMMENT #238=(CGroupWidget COMMENT (CWidget 4 (10 #1# 0) 1 2 0 0 Nil -1223 100) COMMENT (CObjectList COMMENT #239=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #147# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 6.19849 1.9 COMMENT 0.082322 1) COMMENT #240=(CLabel (CWidget 0 (0 0) 1 2 0 0 #139# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (60)" "@N (@S)" 5 3.76528 12.1963 COMMENT 0 -10 3.172 0.799245 #239#) COMMENT #241=(CScratch COMMENT (CWidget 2 (1 #1# 2) 1 2 0 0 #147# 53077736 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 3.95002 1.9 COMMENT 0.082322 1) COMMENT #242=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #139# 160219072 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (1836)" "@N (@S)" 5 11.5584 COMMENT -8.71165 0 -10 3.79641 0.799245 #241#) COMMENT #243=(CScratch COMMENT (CWidget 3 (1 #1# 3) 1 2 0 0 #147# 39963524 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 1.31287 1.9 COMMENT 0.082322 1) COMMENT #244=(CLabel (CWidget 0 (0 0) 1 2 0 0 #139# 1 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (3919)" "@N (@S)" 5 -14.2779 COMMENT 1.74233 0 -10 3.79641 0.799245 #243#) COMMENT #245=(CScratch COMMENT (CWidget 4 (1 #1# 4) 1 2 0 0 #147# 268751352 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.183575 1.9 COMMENT 0.082322 1) COMMENT #246=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #139# 32802 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ava}I (4811)" "@N (@S)" 5 -8.8605 COMMENT 10.454 0 -10 3.69651 0.799245 #245#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #247=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -874 100) COMMENT (CObjectList) (CObjectList)) COMMENT #248=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1363 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 142801790 COMMENT (CStringList) 142801786 2147483648 (CObList))) FEATURES Location/Qualifiers misc_feature 2886..2906 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 2907..2978 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 2915..2949 /vntifkey="39" /label=Stem\loop primer_bind complement(3042..3061) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(3146..3760) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 3161..3161 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 3927..4032 /vntifkey="39" /label=T0\terminator CDS complement(4045..4704) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 4818..4837 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(4894..4897) /vntifkey="39" /label=end\of\terminator stem_loop complement(4907..4926) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix CDS 1219..2885 /vntifkey="4" /label=mutated\version\of\Luciferase/LRE promoter 1..1210 /vntifkey="30" /label=Inducible\pBad/araC\promoter BASE COUNT 1301 a 1215 c 1239 g 1200 t ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtt aaggaggtaa aaaaaatgga gaacatggag aacgacgaga 1261 atattgtggt tggtccgaaa ccgttctacc cgatcgaaga gggcagcgcg ggtacccagc 1321 tgcgtaaata catggaacgc tacgcgaaac tgggtgcgat cgcgttcacg aatgcggtta 1381 ccggcgtgga ctattcctat gcggagtatt tggaaaagtc gtgttgtctg ggcaaggcgt 1441 tacagaatta tggcctggtg gtcgatggcc gtatcgcgct gtgcagcgag aactgtgaag 1501 agtttttcat tccggtgatc gcgggtttgt ttattggcgt gggcgttgca ccgaccaatg 1561 aaatctacac gctgcgcgaa ctggtgcact ccctgggtat ctctaagccg acgatcgttt 1621 tcagctccaa gaaaggtttg gataaagtta ttacggtgca aaagacggtc accacgatca 1681 aaacgattgt gatcctggac agcaaagttg attaccgcgg ttatcaatgc ctggatacgt 1741 tcatcaagcg taacaccccg ccaggttttc aagcatctag cttcaaaacg gtggaggttg 1801 accgtaaaga acaagtcgca ctgattatga atagctcggg tagcacgggc ctgccaaagg 1861 gtgtgcagct gacccatgaa aacaccgtta cccgttttag ccacgcgcgt gatccgattt 1921 acggcaatca agtgtcgccg ggtacggctg ttctgactgt tgtccctttt caccatggtt 1981 ttggcatgtt taccactctg ggttacctga tttgcggttt tcgcgtcgtt atgctgacca 2041 agtttgatga agaaaccttc ctgaaaaccc tgcaagacta caagtgcact aatgtgattc 2101 tggtcccgac gctgttcgcg atcctgaaca aaagcgagct gctgaataag tacgacctga 2161 gcaatttggt ggagattgcg agcggcggtg ctccgttgag caaagaagta ggcgaggcag 2221 tggcacgtcg ctttaacctg cctggcgtcc gtcaaggtta cggtttgact gaaaccacga 2281 gcgcgattat catcaccccg gaaggtgacg ataaacctgg cgctagcggt aaggtcgtgc 2341 cgttgttcaa ggctaaagtt attgatctgg acaccaagaa gagcctgggc ccgaatcgcc 2401 gtggtgaagt ttgcgttaag ggcccgatgc tgatgaaagg ttatgtaaac aatccggaag 2461 caacgaaaga gctgatcgac gaagagggct ggctgcacac tggcgatatc ggctactacg 2521 atgaagagaa acactttttc attgttgatc gcctgaaaag cctgatcaag tacaagggtt 2581 accaagtccc gccagcggag ctggaaagcg tactgttgca gcacccgtcg attttcgacg 2641 ccggtgtggc tggtgtgcct gatccggtgg ctggcgaact gccgggtgct gtcgtggtgc 2701 tggagagcgg taagaatatg accgaaaaag aagtcatgga ctatgtggcg agccaagtgt 2761 ccaatgcaaa acgtctgcgt ggcggtgttc gctttgtgga cgaagtgccg aaaggtctga 2821 ccggcaaaat tgacggtcgt gcaattcgtg agattctgaa gaaaccggtc gcgaaaatgt 2881 aataatacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc 2941 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac 3001 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 3061 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 3121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct 3181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 3241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 3421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 3481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 3541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 3601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 3721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 3781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 3841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 3901 taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa 3961 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg 4021 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg 4081 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat 4141 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg 4201 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg 4261 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc 4321 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta 4381 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg 4441 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc 4501 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt 4561 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc 4621 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt 4681 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc 4741 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg 4801 cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 4861 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta 4921 aggatgattt ctggaattcg cggccgcttc tagag //