LOCUS pSB1C3 9707 bp DNA circular 20-AUG-2017 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|-11904098| COMMENT VNTNAME|pSB1C3| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObList) 1 (CObList)) (CObList) 160515494 COMMENT (CStringList) 160515490 2147484278 (CObList))) FEATURES Location/Qualifiers misc_feature 7638..7658 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 7659..7730 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 7667..7701 /vntifkey="39" /label=Stem\loop primer_bind complement(7794..7813) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(7898..8512) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 7913..7913 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 8679..8784 /vntifkey="39" /label=T0\terminator CDS complement(8797..9456) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 9570..9589 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(9646..9649) /vntifkey="39" /label=end\of\terminator stem_loop complement(9659..9678) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix promoter 1..1079 /vntifkey="30" /label=AraC misc_feature 1080..1210 /vntifkey="21" /label=pBAD RBS 1211..1230 /vntifkey="32" /label=RBC CDS 1231..2660 /vntifkey="4" /label=luxC CDS 2675..3578 /vntifkey="4" /label=luxD CDS 3629..4693 /vntifkey="4" /label=luxA CDS 4732..5712 /vntifkey="4" /label=luxB CDS 5826..6923 /vntifkey="4" /label=luxE CDS 6927..7637 /vntifkey="4" /label=luxG BASE COUNT 3089 a 1761 c 2013 g 2844 t ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc agggaaaata atgaataaat gtattccaat gataattaat ggaatgattc 1261 aagattttga taattatgca tataaagaag ttaaactaaa taatgataat agagtaaaat 1321 tatctgtcat tactgaaagt tcagtttcaa aaacattaaa tatcaaagat agaattaatc 1381 taaatttaaa tcagattgtg aattttttat ataccgttgg tcaacgatgg aaaagtgaag 1441 aatataatcg gcgacgaacc tatatccgtg agttaaaaac atatcttggt tattctgatg 1501 aaatggcaag attagaagcg aattggattg caatgttatt gtgctctaaa agtgctttgt 1561 atgacattgt taattatgat ttgggctcta tacacgtatt agatgaatgg cttccacgtg 1621 gtgattgcta tgttaaagca caaccgaaag gtgtttctgt tcacttgtta gctggtaatg 1681 ttccattatc aggagtgaca tctattttgc gtgctatttt aacaaaaaat gagtgcatta 1741 ttaaaacttc gtcttcagat ccttttactg caaacgcttt agtttccagt tttattgatg 1801 ttaatgcaga ccatccaata accaaatcaa tgtctgttat gtattggccg catgatgaag 1861 atatgactct atctcaaaga ataatgaatc atgccgacgt ggttattgct tggggtggag 1921 acgaggcgat taaatgggcg gtaaaatatt caccaccgca tgtcgatatt ctgaaatttg 1981 gaccaaagaa aagcttaagt attattgaag ctcctaaaga tatagaagcc gcagcaatgg 2041 gggttgctca tgatatttgt ttctatgacc agcaagcctg cttctctact caagacgttt 2101 attatatagg agataattta cctttatttt taaatgaact tgaaaaacag ctagatcgat 2161 acgcgaaaat tttaccaaaa ggttcaaata gttttgatga aaaagcggcg tttactctta 2221 ctgaaaaaga aagtctattt gctggatatg aagtgagaaa gggagataag caagcttggt 2281 taatagtcgt atcacctaca aatagctttg gaaatcaacc gctatcacga agtgtgtatg 2341 ttcatcaagt atctgatatt aaagagataa ttccttttgt taataaaaat agaacacaaa 2401 ctgtttctat ttatccttgg gaagcgtcat taaaatatcg agataaatta gcaagaagtg 2461 gagttgaaag aattgttgaa tcaggcatga ataatatttt cagagttgga ggggctcatg 2521 attcattatc tcctctccag tacctagtta ggtttgtatc gcatgagaga ccatttaatt 2581 atacgacaaa agatgttgcg gttgaaatcg aacaaacacg ttacttagag gaagataagt 2641 ttttagtttt tgtcccatag ttaaaggaaa ttatatgaaa gatgaaagtg ctttttttac 2701 gattgatcac atcatcaagc ttgataatgg tcagtctatc cgagtttggg aaacactccc 2761 taaaaagaac gtaccagaga aaaaacatac aatacttatt gcttcgggtt ttgctagaag 2821 aatggatcat tttgcaggtc ttgctgagta tttatctact aacggttttc atgtcattcg 2881 ctacgattct ttgcatcatg ttggattaag cagtggatgt ataaatgaat ttacgatgtc 2941 gattggaaaa aatagcctgc ttacagtcgt agattggctt acagatcatg gtgtcgaacg 3001 aatagggctg attgctgcta gtttgtcagc gagaatcgcc tatgaggtag taaataaaat 3061 taaattatca tttttaatta cggccgtagg tgtcgttaat cttagagata cattagaaaa 3121 agcattggag tatgactatt tgcaattacc tatttcagag ttaccagaag atcttgactt 3181 tgaaggtcat aatttaggat cggaggtctt tgttacagat tgctttaaac ataattggga 3241 cacgttagac tcgacactta atagtgttaa aggattagcg attccattta ttgcttttac 3301 tgcaaacgat gatagttggg taaagcaaag tgaagttata gagctcattg atagcattga 3361 atctagtaat tgtaagctct attcgctaat tggaagttca catgatcttg gggaaaattt 3421 ggttgtatta agaaattttt atcaatcagt gacgaaggca gccttagcat tagatgatgg 3481 tttattggat ttagagatag acattattga acctcgattt gaggacgtta caagtattac 3541 tgttaaggag cgtagattaa aaaatgaaat tgaaaatgaa ttattagaat tggcttaaat 3601 aaacagaatc accaaaaagg aatagagtat gaagtttgga aatatttgtt tttcgtatca 3661 accaccaggt gaaactcata agcaagtaat ggatcgcttt gttcggcttg gtatcgcctc 3721 agaagaggta gggtttgata catattggac cttagaacat cattttacag agtttggtct 3781 tacgggaaat ttatttgttg ctgcggctaa cctgttagga agaactaaaa cattaaatgt 3841 tggcactatg ggggttgtta ttccgacagc acacccagtt cgacagttag aagacgtttt 3901 attattagat caaatgtcga aaggtcgttt taattttgga accgttcgag ggctatacca 3961 taaagatttt cgagtatttg gtgttgatat ggaagagtct cgagcaatta ctcaaaattt 4021 ctaccagatg ataatggaaa gcttacagac aggaaccatt agctctgata gtgattacat 4081 tcaatttcct aaggttgatg tatatcccaa agtgtactca aaaaatgtac caacctgtat 4141 gactgctgag tccgcaagta cgacagaatg gctagcaata caagggctac caatggttct 4201 tagttggatt attggtacta atgaaaaaaa agcacagatg gaactctata atgaaattgc 4261 gacagaatat ggtcatgata tatctaaaat agatcattgt atgacttata tttgttctgt 4321 tgatgatgat gcacaaaagg cgcaagatgt ttgtcgggag tttctgaaaa attggtatga 4381 ctcatatgta aatgcgacca atatctttaa tgatagcaat caaactcgtg gttatgatta 4441 tcataaaggt caatggcgtg attttgtttt acaaggacat acaaacacca atcgacgtgt 4501 tgattatagc aatggtatta accctgtagg cactcctgag cagtgtattg aaatcattca 4561 acgtgatatt gatgcaacgg gtattacaaa cattacatgc ggatttgaag ctaatggaac 4621 tgaagatgaa ataattgctt ccatgcgacg ctttatgaca caagtcgctc ctttcttaaa 4681 agaacctaaa taaattactt atttgatact agagataata aggaacaagt tatgaaattt 4741 ggattatttt ttctaaactt tcagaaagat ggaataacat ctgaagaaac gttggataat 4801 atggtaaaga ctgtcacgtt aattgattca actaaatatc attttaatac tgcctttgtt 4861 aatgaacatc acttttcaaa aaatggtatt gttggagcac ctattaccgc agctggtttt 4921 ttattagggt taacaaataa attacatatt ggttcattaa atcaagtaat taccacccat 4981 caccctgtac gtgtagcaga agaagccagt ttattagatc aaatgtcaga gggacgcttc 5041 attcttggtt ttagtgactg cgaaagtgat ttcgaaatgg aattttttag acgtcatatc 5101 tcatcaaggc aacaacaatt tgaagcatgc tatgaaataa ttaatgacgc attaactaca 5161 ggttattgtc atccccaaaa cgacttttat gattttccaa aggtttcaat taatccacac 5221 tgttacagtg agaatggacc taagcaatat gtatccgcta catcaaaaga agtcgtcatg 5281 tgggcagcga aaaaggcact gcctttaaca tttaagtggg aggataattt agaaaccaaa 5341 gaacgctatg caattctata taataaaaca gcacaacaat atggtattga tatttcggat 5401 gttgatcatc aattaactgt aattgcgaac ttaaatgctg atagaagtac ggctcaagaa 5461 gaagtgagag aatacttaaa agactatatc actgaaactt accctcaaat ggacagagat 5521 gaaaaaatta actgcattat tgaagagaat gcagttgggt ctcatgatga ctattatgaa 5581 tcgacaaaat tagcagtgga aaaaacaggg tctaaaaata ttttattatc ctttgaatca 5641 atgtccgata ttaaagatgt aaaagatatt attgatatgt tgaaccaaaa aatcgaaatg 5701 aatttaccat aataaaatta aaggcaattt ctatattaga ttgccttttt aaatttctgt 5761 tgatattagg tattactgga gagggtatga ctgtccatac tgaatataaa agaaatcaaa 5821 tcattgctag ttcagaaatt gatgatctta tctttatgac gaaaccacaa gagtggtcat 5881 ttgaagagca aaaagaaata cgggataaat tagttcgtga ggctttttat tttcactaca 5941 atagaaatga agaatataga aattattgta tcaatcagca tgtgagtgat aatttacaca 6001 ctattgatga aatacccgtg tttccaacat ctgtttttaa atataagaaa ttacatactg 6061 tcacagccga ggacattgaa aattggtata caagtagtgg aactcgtgga gtaaaaagtc 6121 atattgcacg tgatcgtctt agcattgaac gcttgcttgg ttctgtcaac ttcggaatga 6181 aatacgttgg agattggttt gagcatcaaa tggaattgat aaatttagga ccagatagat 6241 tcaatacaaa taatatttgg tttaaatatg tcatgagttt ggtcgagtta ctttatccga 6301 ctgaatttac agttgataat gacaaaatag attttgaaaa aacagtaaaa catctattta 6361 gaattaagaa tagtaaaaaa gacatttgct taattgggcc accatttttt gtgtatcttt 6421 tgtgccaata tatgaaagaa aacaatattg aatttaaagg aggagataga gtacatatta 6481 ttactggtgg aggatggaaa tctaatcaga atgactcttt agatcgtgct gattttaatc 6541 aattattaat ggatactttc caactcgaca agattaatca aattagagat acctttaatc 6601 aagttgagct taatacttgt ttttttgaag atgaatttca aagaaaacat gttccaccgt 6661 gggtatatgc tcgggctctt gatcctgaaa ccttgaaacc cgtagcagat ggtgagatcg 6721 ggttgttaag ttatatggac gcctcatcaa ctgcttaccc tgcttttatt gttactgatg 6781 atatcggtat tgtaaaagaa attagagaac cagatcctta cccaggggta actgttgaga 6841 ttgttcggcg cttaaataca cgtgcgcaaa aaggatgcgc gctctctatg gctaatgtca 6901 tacaaaagaa tatcaaggat taagttatga ttgttgatgg tagagtttca aaaatagtat 6961 tagcgtcaat aagaaataat atatataagg tatttattac tgtaaattca ccaataaagt 7021 tcatcgctgg acaatttgta atggtcacga ttaatgggaa aaaatgccct ttttcaattg 7081 cgaattgccc gacaaaaaat tacgaaatag aattgcatat tggtagttcg aatagagact 7141 gctcattgga tattatcgaa tattttgtcg atgctcttgt tgaggaagtc gcaattgagt 7201 tagatgctcc ccatggaaac gcttggttac ggtctgaaag taataaccca ttgctattaa 7261 ttgcgggagg tacaggttta tcatatataa atagcattct aacaaattgc ttaaatagga 7321 atatacctca agatatttat ctttactggg gagtaaaaga cagttctctt ttgtatgaag 7381 atgaagagtt actaaactta tcactaaaca acaaaaactt tcattatatt cctgttattg 7441 aagataaaag tgaagaatgg atagggagaa aaggcactgt tcttgatgct gtcatggaag 7501 attttactga tcttacttat tttgatattt atgtttgtgg acccttcatg atggctaaaa 7561 cagcaaaaga aaaattaatt gaagagaaaa aagcaaagtc agaacagatg tttgccgatg 7621 cttttgcata cgtataatac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg 7681 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc 7741 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 7801 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 7861 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg 7921 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 7981 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 8041 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 8101 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 8161 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 8221 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 8281 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 8341 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 8401 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 8461 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 8521 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 8581 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 8641 agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac 8701 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga 8761 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc 8821 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg 8881 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat 8941 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa 9001 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata 9061 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa 9121 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt 9181 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa 9241 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg 9301 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata 9361 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat 9421 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa 9481 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga 9541 acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca 9601 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct 9661 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagag //