LOCUS pSB1C3 5792 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 5792 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 5792 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 3743 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 5787 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 5772 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 1 3725 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 3723 3743 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 3744 3815 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 3752 3786 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 3879 3898 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 3983 4597 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 3998 3998 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 4764 4869 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 4882 5541 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 5655 5674 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 5731 5734 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 5744 5763 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_K415023" 21 0 0 1 3722 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "plux-lac hybrid promoter" 30 0 0 1 74 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) "RBS.1 (strong) -- modified from R. Weiss" 32 0 COMMENT 0 83 97 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #18=(CFSignal (CObList) COMMENT "green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP" COMMENT 21 0 0 104 823 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #19=(CFSignal (CObList) "T1 from E. coli rrnB" 43 0 0 2820 2899 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #20=(CFSignal (CObList) "T1 from E. coli rrnB" 43 0 0 1814 1893 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #21=(CFSignal (CObList) "T1 from E. coli rrnB" 43 0 0 832 911 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #22=(CFSignal (CObList) "TE from coliphage T7" 43 0 0 2908 2948 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #23=(CFSignal (CObList) "TE from coliphage T7" 43 0 0 920 960 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #24=(CFSignal (CObList) "TE from coliphage T7" 43 0 0 1902 1942 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #25=(CFSignal (CObList) COMMENT "Promoter (lambda cI and luxR regulated -- hybrid)" 29 0 0 2957 COMMENT 3053 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #26=(CFSignal (CObList) COMMENT "Promoter (lambda cI and luxR regulated -- hybrid)" 29 0 0 969 1065 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #27=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 86 97 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #28=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 1074 1085 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #29=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 2013 2024 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #30=(CFSignal (CObList) "monomeric RFP optimized for bacteria" 21 0 0 COMMENT 1092 1805 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #31=(CFSignal (CObList) "TetR repressible promoter" 30 0 0 1951 2004 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #32=(CFSignal (CObList) "luxR repressor/activator, (no LVA?)" 21 0 0 COMMENT 2031 2811 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #33=(CFSignal (CObList) "autoinducer synthetase for AHL" 21 0 0 3080 COMMENT 3722 0 (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #34=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #35=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #36=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" COMMENT 1) COMMENT #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Creation Date: ? (no data)" 1) COMMENT #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modification Date: ? (no data)" 1) COMMENT #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 5792 bp" 1) COMMENT #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #45=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Associated Genes: rep (pMB1); ORI; Chloramphenicol resistance" COMMENT 1))) "Standard Fields") COMMENT #48=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #49=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #50=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #51=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #52=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4882 (3 #11# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #53=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4882 End: 5541 (Complementary)" COMMENT 1) COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1160..1819)" 1) COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"Chloramphenicol resistance\"" COMMENT 1))) "Chloramphenicol resistance"))) COMMENT "CDS (1 total)") COMMENT #58=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #59=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 3722" 1))) COMMENT "BBa_K415023") COMMENT #61=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 104 (3 #18# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #62=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 104 End: 823 " 1))) COMMENT "green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP") COMMENT #63=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1092 (3 #30# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1092 End: 1805" 1))) COMMENT "monomeric RFP optimized for bacteria") COMMENT #65=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2031 (3 #32# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #66=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2031 End: 2811" 1))) COMMENT "luxR repressor/activator, (no LVA?)") COMMENT #67=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3080 (3 #33# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3080 End: 3722" 1))) COMMENT "autoinducer synthetase for AHL") COMMENT #69=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3723 (3 #4# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3723 End: 3743" 1) COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1..21" 1))) "DNA: BioBrick suffix") COMMENT #73=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3983 (3 #8# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #74=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3983 End: 4597 (Complementary)" COMMENT 1) COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(261..875)" 1) COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"rep (pMB1)\"" 1))) COMMENT "rep (pMB1)") COMMENT #79=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3998 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3998 End: 3998" 1) COMMENT #81=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 276..276" 1) COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"ORI\"" 1))) "ORI"))) COMMENT "Misc. Feature (8 total)") COMMENT #85=(CFolderPar COMMENT (CGroupPar (CParagraph 28 (7 28 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #86=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3879 (3 #7# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3879 End: 3898 (Complementary)" COMMENT 1) COMMENT #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #89=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(157..176)" 1))) COMMENT "Verification reverse (VR) primer binding site") COMMENT #90=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5655 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #91=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5655 End: 5674" 1) COMMENT #92=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #93=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1933..1952" 1))) COMMENT "Verification forward (VF2) primer binding site"))) COMMENT "Primer Binding Site (2 total)") COMMENT #94=(CFolderPar COMMENT (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #95=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 969 (3 #26# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #96=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 969 End: 1065" 1))) COMMENT "Promoter (lambda cI and luxR regulated -- hybrid)") COMMENT #97=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2957 (3 #25# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #98=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2957 End: 3053" 1))) COMMENT "Promoter (lambda cI and luxR regulated -- hybrid)"))) COMMENT "Promoter Eukaryotic (2 total)") COMMENT #99=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #100=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #101=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 74 " 1))) COMMENT "plux-lac hybrid promoter") COMMENT #102=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1951 (3 #31# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #103=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1951 End: 2004" 1))) COMMENT "TetR repressible promoter"))) COMMENT "Promoter Prokaryotic (2 total)") COMMENT #104=(CFolderPar COMMENT (CGroupPar (CParagraph 32 (7 32 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #105=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 83 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #106=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 83 End: 97 " 1))) COMMENT "RBS.1 (strong) -- modified from R. Weiss") COMMENT #107=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 86 (3 #27# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #108=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 86 End: 97 " 1))) COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency") COMMENT #109=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1074 (3 #28# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #110=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1074 End: 1085" 1))) COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency") COMMENT #111=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2013 (3 #29# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #112=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2013 End: 2024" 1))) COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency"))) COMMENT "RBS (4 total)") COMMENT #113=(CFolderPar COMMENT (CGroupPar (CParagraph 39 (7 39 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #114=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3744 (3 #5# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #115=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3744 End: 3815" 1) COMMENT #116=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #117=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 22..93" 1))) COMMENT "E. coli his operon terminator") COMMENT #118=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3752 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #119=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3752 End: 3786" 1) COMMENT #120=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #121=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 30..64" 1))) "Stem loop") COMMENT #122=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 4764 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #123=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 4764 End: 4869" 1) COMMENT #124=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #125=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1042..1147" 1))) "T0 terminator") COMMENT #126=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5731 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #127=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5731 End: 5734 (Complementary)" COMMENT 1) COMMENT #128=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #129=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2009..2012)" 1))) COMMENT "end of terminator") COMMENT #130=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 5744 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #131=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 5744 End: 5763 (Complementary)" COMMENT 1) COMMENT #132=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #133=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2022..2041)" 1))) COMMENT "Stem loop"))) "Stem Loop (5 total)") COMMENT #134=(CFolderPar COMMENT (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #135=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 832 (3 #21# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #136=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 832 End: 911 " 1))) COMMENT "T1 from E. coli rrnB") COMMENT #137=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 920 (3 #23# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #138=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 920 End: 960 " 1))) COMMENT "TE from coliphage T7") COMMENT #139=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1814 (3 #20# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #140=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1814 End: 1893" 1))) COMMENT "T1 from E. coli rrnB") COMMENT #141=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1902 (3 #24# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #142=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1902 End: 1942" 1))) COMMENT "TE from coliphage T7") COMMENT #143=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2820 (3 #19# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #144=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2820 End: 2899" 1))) COMMENT "T1 from E. coli rrnB") COMMENT #145=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2908 (3 #22# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #146=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2908 End: 2948" 1))) COMMENT "TE from coliphage T7"))) COMMENT "Terminator (6 total)"))) "Feature Map") COMMENT #147=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #148=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #149=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #150=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 0 End: 0 " 1) COMMENT #151=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #152=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 0..0" 1))) COMMENT "DNA: BioBrick prefix"))) COMMENT "Misc. Feature (1 total)"))) COMMENT "Imported Features Not Shown on Map") COMMENT #153=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #154=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39466120 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #155=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #156=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 3725 " 1))))) "AhlI: 1 site") COMMENT 1 5 "ACTAGT" "TGATCA") COMMENT #157=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39478728 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #158=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #159=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 5772 " 1))))) "EcoRI: 1 site") COMMENT 1 5 "GAATTC" "CTTAAG") COMMENT #160=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39478408 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #161=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #162=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 3743 " 1))))) "PstI: 1 site") COMMENT 5 1 "CTGCAG" "GACGTC") COMMENT #163=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39478568 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #164=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #165=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 5787 " 1))))) "XbaI: 1 site") COMMENT 1 5 "TCTAGA" "AGATCT"))) COMMENT "Restriction/Methylation Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #166=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #167=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #168=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #169=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #170=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #171=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #172=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #173=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #174=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #175=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #176=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #177=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #178=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #179=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #180=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #181=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #182=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #183=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #184=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #185=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #186=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #187=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #188=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #189=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #190=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #191=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #192=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #193=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #194=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #195=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #196=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #197=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #198=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #199=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #200=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #201=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #202=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #203=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #204=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #205=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 COMMENT -2070) 1 1 0 1 1 COMMENT (mapper: 32.7045 -17.8387 40.0378 40.0378 0.01 10 -1 5792 5792 1 0 0) COMMENT #206=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -1148 100) COMMENT (CObjectList COMMENT #207=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -316 100) COMMENT (CObjectList COMMENT #208=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #172# 40529340 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27527 6.27327 1 0.0214037) 0.108308) COMMENT #209=(CLabel (CWidget 1001 (0 0) 1 2 0 0 #169# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "pSB1C3" "@N" 1 0 0.871165 0 -10 COMMENT 4.49575 1.39868 Nil) COMMENT #210=(CLabel (CWidget 1002 (0 0) 1 2 0 0 #168# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "5792 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 2.52262 0.799245 Nil)) (CObjectList)) COMMENT #211=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -246 100) COMMENT (CObjectList COMMENT #212=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -1223 100) COMMENT (CObjectList COMMENT #213=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #170# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 0.271852 0.986684 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #214=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #167# -858993459 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Chloramphenicol resistance" "@N" 1 -16.1295 COMMENT 6.96932 0 -10 12.2634 1.04901 #213#)) COMMENT (CObjectList)) COMMENT #215=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil -1084 100) COMMENT (CObjectList COMMENT #216=(CScratch COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #175# 39694240 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 2.24197 1.9 COMMENT 0.082322 1) COMMENT #217=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "DNA: BioBrick suffix" "@N" 1 -14.2531 COMMENT -8.71165 0 -10 8.81667 1.04901 #216#) COMMENT #218=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #8# 0) 1 2 0 0 #171# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 1.29428 1.96037 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #219=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "rep (pMB1)" COMMENT "@N" 1 -14.441 3.48466 0 -10 4.77049 1.04901 COMMENT #218#) COMMENT #220=(CScratch COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #175# 39713512 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 1.94412 1.9 COMMENT 0.082322 1) COMMENT #221=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "ORI" "@N" 1 COMMENT -12.6923 1.74233 0 -10 1.67342 1.04901 #220#) COMMENT #222=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #171# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 2.24197 COMMENT 6.27319 1 0.082322) 0.8 1.8 0) COMMENT #223=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "BBa K415023" COMMENT "@N" 1 13.8055 -5.22699 0 -10 5.91941 1.04901 COMMENT #222#) COMMENT #224=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #171# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 5.38181 6.16163 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #225=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP" COMMENT "@N" 1 25.8201 8.71165 0 -10 34.7172 1.04901 COMMENT #224#) COMMENT #226=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #30# 0) 1 2 0 0 #171# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 4.31823 5.09155 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #227=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "monomeric RFP optimized for bacteria" "@N" 1 COMMENT 20.2344 0 0 -10 16.5843 1.04901 #226#) COMMENT #228=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #32# 0) 1 2 0 0 #171# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 3.22865 4.07454 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #229=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #167# 13434879 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "luxR repressor/activator, (no LVA?)" "@N" 1 COMMENT 14.2001 -12.1963 0 -10 15.3355 1.04901 #228#) COMMENT #230=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #33# 0) 1 2 0 0 #171# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.670712 2.24197 COMMENT 2.93839 1 0.082322) 0.8 1.8 0) COMMENT #231=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "autoinducer synthetase for AHL" "@N" 1 COMMENT -13.7613 -10.454 0 -10 13.5872 1.04901 #230#)) COMMENT (CObjectList)) COMMENT #232=(CGroupWidget COMMENT (CWidget 28 (7 28 0) 1 2 0 0 Nil -595 100) COMMENT (CObjectList COMMENT #233=(CScratch COMMENT (CWidget 0 (3 #7# 0) 1 2 0 0 #188# 34026984 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 2.07301 1.9 COMMENT 0.082322 1) COMMENT #234=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification reverse (VR) primer binding site" COMMENT "@N" 1 -21.3756 0 0 -10 19.1319 1.04901 #233#) COMMENT #235=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #188# 39700424 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.149465 1.9 COMMENT 0.082322 1) COMMENT #236=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification forward (VF2) primer binding site" COMMENT "@N" 1 -18.3804 8.71165 0 -10 19.4816 1.04901 COMMENT #235#)) (CObjectList)) COMMENT #237=(CGroupWidget COMMENT (CWidget 29 (7 29 0) 1 2 0 0 Nil -735 100) COMMENT (CObjectList COMMENT #238=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #25# 0) 1 2 0 0 #171# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.96655 3.07161 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #239=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Promoter (lambda cI and luxR regulated -- hybrid)" COMMENT "@N" 1 -12.8664 -12.1963 0 -10 21.155 1.04901 COMMENT #238#) COMMENT #240=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #26# 0) 1 2 0 0 #171# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 5.11971 5.22477 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #241=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Promoter (lambda cI and luxR regulated -- hybrid)" COMMENT "@N" 1 22.1832 3.48466 0 -10 21.155 1.04901 COMMENT #240#)) (CObjectList)) COMMENT #242=(CGroupWidget COMMENT (CWidget 30 (7 30 0) 1 2 0 0 Nil -386 100) COMMENT (CObjectList COMMENT #243=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #183# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 COMMENT 6.19304 6.27319 1 0.082322) 0.8 1.8 0) COMMENT #244=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "plux-lac hybrid promoter" "@N" 1 6.85616 COMMENT 13.9386 0 -10 10.59 1.04901 #243#) COMMENT #245=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #31# 0) 1 2 0 0 #183# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 4.1027 COMMENT 4.16118 1 0.082322) 0.8 1.8 0) COMMENT #246=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "TetR repressible promoter" "@N" 1 16.1874 COMMENT -8.71165 0 -10 11.2893 1.04901 #245#)) COMMENT (CObjectList)) COMMENT #247=(CGroupWidget COMMENT (CWidget 32 (7 32 0) 1 2 0 0 Nil -804 100) COMMENT (CObjectList COMMENT #248=(CScratch COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #187# 39704768 COMMENT 100) (LOGPEN 0 6 16711680) 8 1 6.18437 1.9 COMMENT 0.082322 1) COMMENT #249=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "RBS.1 (strong) -- modified from R. Weiss" COMMENT "@N" 1 10.6155 12.1963 0 -10 17.2837 1.04901 COMMENT #248#) COMMENT #250=(CScratch COMMENT (CWidget 0 (3 #27# 0) 1 2 0 0 #187# 39694560 COMMENT 100) (LOGPEN 0 6 16711680) 8 1 6.18112 1.9 COMMENT 0.082322 1) COMMENT #251=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT "@N" 1 15.7863 10.454 0 -10 19.032 1.04901 COMMENT #250#) COMMENT #252=(CScratch COMMENT (CWidget 0 (3 #28# 0) 1 2 0 0 #187# 39694880 COMMENT 100) (LOGPEN 0 6 16711680) 8 1 5.11104 1.9 COMMENT 0.082322 1) COMMENT #253=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT "@N" 1 21.1775 1.74233 0 -10 19.032 1.04901 COMMENT #252#) COMMENT #254=(CScratch COMMENT (CWidget 0 (3 #29# 0) 1 2 0 0 #187# 33994968 COMMENT 100) (LOGPEN 0 6 16711680) 8 1 4.09403 1.9 COMMENT 0.082322 1) COMMENT #255=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT "@N" 1 19.7022 -10.454 0 -10 19.032 1.04901 COMMENT #254#)) (CObjectList)) COMMENT #256=(CGroupWidget COMMENT (CWidget 39 (7 39 0) 1 2 0 0 Nil -177 100) COMMENT (CObjectList COMMENT #257=(CLine COMMENT (CWidget 0 (3 #5# 0) 1 2 0 0 #188# 2114 100) COMMENT (LOGPEN 0 0 16711680) 1 0.835356 2.14124 COMMENT 2.21923) COMMENT #258=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "E. coli his operon terminator" "@N" 1 COMMENT -18.0152 -1.74233 0 -10 12.3383 1.04901 #257#) COMMENT #259=(CScratch COMMENT (CWidget 0 (3 #6# 0) 1 2 0 0 #188# 39698744 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 2.21056 1.9 COMMENT 0.082322 1) COMMENT #260=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Stem loop" "@N" COMMENT 1 -13.759 -3.48466 0 -10 4.42082 1.04901 COMMENT #259#) COMMENT #261=(CLine COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #188# 6553635 COMMENT 100) (LOGPEN 0 0 16711680) 1 0.835356 COMMENT 0.999681 1.11449) COMMENT #262=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T0 terminator" COMMENT "@N" 1 -13.9275 5.22699 0 -10 5.74457 1.04901 COMMENT #261#) COMMENT #263=(CScratch COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #188# 33991112 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.0671508 1.9 COMMENT 0.082322 1) COMMENT #264=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "end of terminator" "@N" 1 -10.2544 10.454 0 COMMENT -10 7.41799 1.04901 #263#) COMMENT #265=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #188# 39699064 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.0530708 1.9 COMMENT 0.082322 1) COMMENT #266=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Stem loop" "@N" COMMENT 1 -3.61397 12.1963 0 -10 4.42082 1.04901 COMMENT #265#)) (CObjectList)) COMMENT #267=(CGroupWidget COMMENT (CWidget 43 (7 43 0) 1 2 0 0 Nil -1223 100) COMMENT (CObjectList COMMENT #268=(CLine COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #175# -283 100) COMMENT (LOGPEN 0 8 13395507) 10 0.670712 3.13334 COMMENT 3.21999) COMMENT #269=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "T1 from E. coli rrnB" "@N" 1 5.50933 COMMENT -13.9386 0 -10 8.39207 1.04901 #268#) COMMENT #270=(CLine COMMENT (CWidget 0 (3 #20# 0) 1 2 0 0 #175# 8 100) COMMENT (LOGPEN 0 8 13395507) 10 0.670712 4.22292 COMMENT 4.30957) COMMENT #271=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "T1 from E. coli rrnB" "@N" 1 15.754 -3.48466 COMMENT 0 -10 8.39207 1.04901 #270#) COMMENT #272=(CLine COMMENT (CWidget 0 (3 #21# 0) 1 2 0 0 #175# -944 100) COMMENT (LOGPEN 0 8 13395507) 10 0.670712 5.2865 COMMENT 5.37315) COMMENT #273=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "T1 from E. coli rrnB" "@N" 1 14.3084 6.96932 COMMENT 0 -10 8.39207 1.04901 #272#) COMMENT #274=(CScratch COMMENT (CWidget 0 (3 #22# 0) 1 2 0 0 #175# 39698744 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 3.12468 1.9 COMMENT 0.082322 1) COMMENT #275=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "TE from coliphage T7" "@N" 1 -5.69266 COMMENT -13.9386 0 -10 9.09141 1.04901 #274#) COMMENT #276=(CScratch COMMENT (CWidget 0 (3 #23# 0) 1 2 0 0 #175# 39698824 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 5.27784 1.9 COMMENT 0.082322 1) COMMENT #277=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "TE from coliphage T7" "@N" 1 15.5827 5.22699 COMMENT 0 -10 9.09141 1.04901 #276#) COMMENT #278=(CScratch COMMENT (CWidget 0 (3 #24# 0) 1 2 0 0 #175# 39706608 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 4.21425 1.9 COMMENT 0.082322 1) COMMENT #279=(CLabel (CWidget 0 (0 0) 1 2 0 0 #167# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "TE from coliphage T7" "@N" 1 15.4649 COMMENT -6.96932 0 -10 9.09141 1.04901 #278#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #280=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -386 100) COMMENT (CObjectList COMMENT #281=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -944 100) COMMENT (CObjectList COMMENT #282=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #174# 39694960 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 2.22031 1.9 COMMENT 0.082322 1) COMMENT #283=(CLabel (CWidget 0 (0 0) 1 2 0 0 #176# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Pst}I (3743)" "@N (@S)" 5 -13.2306 COMMENT -5.22699 0 -10 3.54665 0.799245 #282#)) COMMENT (CObjectList)) COMMENT #284=(CGroupWidget COMMENT (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -735 100) COMMENT (CObjectList COMMENT #285=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #174# 39700904 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.00649847 COMMENT 1.9 0.082322 1) COMMENT #286=(CLabel (CWidget 0 (0 0) 1 2 0 0 #176# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Xba}I (5787)" "@N (@S)" 5 -3.52716 COMMENT 15.681 0 -10 3.89632 0.799245 #285#)) COMMENT (CObjectList)) COMMENT #287=(CGroupWidget COMMENT (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -665 100) COMMENT (CObjectList COMMENT #288=(CScratch COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #174# 39696720 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.0227446 1.9 COMMENT 0.082322 1) COMMENT #289=(CLabel (CWidget 0 (0 0) 1 2 0 0 #176# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Eco}RI (5772)" "@N (@S)" 5 -3.84591 COMMENT 13.9386 0 -10 4.14608 0.799245 #288#)) COMMENT (CObjectList)) COMMENT #290=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -1153 100) COMMENT (CObjectList COMMENT #291=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #174# 39711032 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 2.2398 1.9 COMMENT 0.082322 1) COMMENT #292=(CLabel (CWidget 0 (0 0) 1 2 0 0 #176# 1 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Ahl}I (3725)" "@N (@S)" 5 -12.4271 COMMENT -6.96932 0 -10 3.52167 0.799245 #291#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #293=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -456 100) COMMENT (CObjectList) (CObjectList)) COMMENT #294=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -765 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 3723..3743 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 3744..3815 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 3752..3786 /vntifkey="39" /label=Stem\loop primer_bind complement(3879..3898) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(3983..4597) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 3998..3998 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 4764..4869 /vntifkey="39" /label=T0\terminator CDS complement(4882..5541) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 5655..5674 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(5731..5734) /vntifkey="39" /label=end\of\terminator stem_loop complement(5744..5763) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..3722 /vntifkey="21" /label=BBa_K415023 promoter 1..74 /vntifkey="30" /label=plux-lac\hybrid\promoter RBS 83..97 /vntifkey="32" /label=RBS.1\(strong)\--\modified\from\R.\Weiss misc_feature 104..823 /vntifkey="21" /label=green\fluorescent\protein\derived\from\jellyfish\Aequeora\victoria\wild-type\GFP terminator 2820..2899 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 1814..1893 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 832..911 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 2908..2948 /vntifkey="43" /label=TE\from\coliphage\T7 terminator 920..960 /vntifkey="43" /label=TE\from\coliphage\T7 terminator 1902..1942 /vntifkey="43" /label=TE\from\coliphage\T7 promoter 2957..3053 /vntifkey="29" /label=Promoter\(lambda\cI\and\luxR\regulated\--\hybrid) promoter 969..1065 /vntifkey="29" /label=Promoter\(lambda\cI\and\luxR\regulated\--\hybrid) RBS 86..97 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency RBS 1074..1085 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency RBS 2013..2024 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency misc_feature 1092..1805 /vntifkey="21" /label=monomeric\RFP\optimized\for\bacteria promoter 1951..2004 /vntifkey="30" /label=TetR\repressible\promoter misc_feature 2031..2811 /vntifkey="21" /label=luxR\repressor/activator,\(no\LVA?) misc_feature 3080..3722 /vntifkey="21" /label=autoinducer\synthetase\for\AHL BASE COUNT 1761 a 1241 c 1292 g 1498 t ORIGIN 1 acctgtagga tcgtacaggt ttacttgtga gcggataaca atatagtgtg tggaattgtg 61 agcggataac aatttactag agattaaaga ggagaaatac tagatgcgta aaggagaaga 121 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa 181 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt 241 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta 301 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag 361 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta 421 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa 481 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa 541 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa 601 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac 661 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc 721 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc 781 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gccaggcatc 841 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 901 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 961 tactagagta agcacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt 1021 cgaataacac cgtgcgtgtt gactatttta cctctggcgg tgatatacta gagaaagagg 1081 agaaatacta gatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca 1141 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg 1201 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg 1261 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct 1321 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca 1381 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct 1441 ccttgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg 1501 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc 1561 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc 1621 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg 1681 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg 1741 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt 1801 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 1861 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 1921 ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat tgacatccct 1981 atcagtgata gagatactga gcactactag agaaagagga gaaatactag atgaaaaaca 2041 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg 2101 atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg 2161 cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta 2221 aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt 2281 attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata 2341 aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt 2401 tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag 2461 acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt 2521 ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa 2581 ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt 2641 caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga 2701 aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg 2761 attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc 2821 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 2881 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 2941 cgtttatata ctagagtaag cacctgtagg atcgtacagg tttacgcaag aaaatggttt 3001 gttatagtcg aataacaccg tgcgtgttga ctattttacc tctggcggtg atatactaga 3061 gaaagaggag aaatactaga tgactataat gataaaaaaa tcggattttt tggcaattcc 3121 atcggaggag tataaaggta ttctaagtct tcgttatcaa gtgtttaagc aaagacttga 3181 gtgggactta gttgtagaaa ataaccttga atcagatgag tatgataact caaatgcaga 3241 atatatttat gcttgtgatg atactgaaaa tgtaagtgga tgctggcgtt tattacctac 3301 aacaggtgat tatatgctga aaagtgtttt tcctgaattg cttggtcaac agagtgctcc 3361 caaagatcct aatatagtcg aattaagtcg ttttgctgta ggtaaaaata gctcaaagat 3421 aaataactct gctagtgaaa ttacaatgaa actatttgaa gctatatata aacacgctgt 3481 tagtcaaggt attacagaat atgtaacagt aacatcaaca gcaatagagc gatttttaaa 3541 gcgtattaaa gttccttgtc atcgtattgg agacaaagaa attcatgtat taggtgatac 3601 taaatcggtt gtattgtcta tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc 3661 tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc 3721 tctactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct 3781 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 3841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 3901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 3961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac 4021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 4081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 4141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 4201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 4261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 4321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 4381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 4441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 4501 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 4561 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 4621 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 4681 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 4741 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg 4801 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 4861 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa 4921 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc 4981 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc 5041 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt 5101 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc 5161 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc 5221 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac 5281 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt 5341 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac 5401 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac 5461 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata 5521 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa 5581 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc 5641 gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 5701 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg 5761 atgatttctg gaattcgcgg ccgcttctag ag //