LOCUS BBa_K51603 2934 bp DNA circular 20-AUG-2017 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-11904162| COMMENT VNTDBDATE|-11904167| COMMENT VNTNAME|BBa_K516030| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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/label=E.\coli\his\operon\terminator stem_loop 894..928 /vntifkey="39" /label=Stem\loop primer_bind complement(1021..1040) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1125..1739) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1140..1140 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1906..2011 /vntifkey="39" /label=T0\terminator CDS complement(2024..2683) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2797..2816 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2873..2876) /vntifkey="39" /label=end\of\terminator stem_loop complement(2886..2905) /vntifkey="39" /label=Stem\loop misc_feature 2051..2072 /vntifkey="21" /label=DNA:\BioBrick\prefix RBS 1..15 /vntifkey="32" /label=RBS.1\(strong)\--\modified\from\R.\Weiss CDS 22..696 /vntifkey="4" /label=mRFP /note="E1010" misc_signal 697..727 /vntifkey="87" /label=Barcode polyA_signal 851..864 /vntifkey="25" /label=polyA /note="B0012" misc_feature 1..864 /vntifkey="21" /label=BBa_K516030 misc_feature 22..727 /vntifkey="21" /label=**highly**\engineered\mutant\of\red\fluorescent\protein\from\Discosoma\striata\(coral) terminator 736..815 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 824..864 /vntifkey="43" /label=TE\from\coliphageT7 BASE COUNT 806 a 719 c 689 g 720 t ORIGIN 1 attaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt 61 ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt 121 gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg 181 ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt 241 aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg 301 gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg 361 caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt 421 ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa 481 gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac 541 gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac 601 aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag 661 tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt 721 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 781 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 841 cgggtgggcc tttctgcgtt tatatactag tagcggccgc tgcagtccgg caaaaaaggg 901 caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca 961 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 1021 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 1081 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 1141 ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 1201 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 1261 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 1321 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 1381 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 1441 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 1501 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 1561 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 1621 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 1681 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 1741 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 1801 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 1861 aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat 1921 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat 1981 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc 2041 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa 2101 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt 2161 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac 2221 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag 2281 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact 2341 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga 2401 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca 2461 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa 2521 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct 2581 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt 2641 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc 2701 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa 2761 agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt 2821 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa 2881 aaatccttag ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agag //