LOCUS pSB1C3 2975 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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"{\\i Ahl}I (908)" "@N (@S)" 5 13.5502 COMMENT -5.22699 0 -10 3.44674 0.799245 #224#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #226=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1363 100) COMMENT (CObjectList) (CObjectList)) COMMENT #227=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -874 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 906..926 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 927..998 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 935..969 /vntifkey="39" /label=Stem\loop primer_bind complement(1062..1081) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1166..1780) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1181..1181 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1947..2052 /vntifkey="39" /label=T0\terminator CDS complement(2065..2724) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2838..2857 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2914..2917) /vntifkey="39" /label=end\of\terminator stem_loop complement(2927..2946) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..905 /vntifkey="21" /label=BBa_K516132 promoter 1..35 /vntifkey="29" /label=constitutive\promoter\family\member RBS 44..56 /vntifkey="32" /label=RBS.3\(medium)\--\derivative\of\BBa_0030 misc_feature 63..768 /vntifkey="21" /label=**highly**\engineered\mutant\of\red\fluorescent\protein\from\Discosoma\striata\(coral) terminator 777..856 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 865..905 /vntifkey="43" /label=TE\from\coliphage\T7 BASE COUNT 814 a 731 c 697 g 733 t ORIGIN 1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact 61 agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag 121 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag 181 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca 241 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc 301 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg 361 aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct 421 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa 481 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg 541 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca 601 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg 661 acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc 721 gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag 781 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 841 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 901 ttatatacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc 961 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac 1021 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 1081 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 1141 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct 1201 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 1261 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 1321 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 1381 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 1441 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 1501 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 1561 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 1621 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1681 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 1741 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 1801 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 1861 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1921 taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa 1981 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg 2041 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg 2101 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat 2161 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg 2221 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg 2281 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc 2341 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta 2401 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg 2461 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc 2521 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt 2581 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc 2641 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt 2701 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc 2761 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg 2821 cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 2881 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta 2941 aggatgattt ctggaattcg cggccgcttc tagag //