LOCUS pSB1C3 4047 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 4047 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 4047 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1998 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 4042 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4027 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 1 1980 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 1978 1998 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 1999 2070 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 2007 2041 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 2134 2153 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 2238 2852 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 2253 2253 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 3019 3124 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 3137 3796 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 3910 3929 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 3986 3989 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 3999 4018 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_K523015" 21 0 0 1 1977 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "lac promoter and lacZ" 30 0 0 1 600 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) COMMENT "strong synthetic E. coli ribosome binding site with SacI site." 32 COMMENT 0 0 609 618 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #18=(CFSignal (CObList) COMMENT "cenA coding sequence encoding Cellulomonas fimi endoglucanase A" COMMENT 21 0 0 625 1977 0 (CStringList) (CStringList) 1 1 1 1 "")) COMMENT (CObList) (CObList) (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #19=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #20=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #21=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. 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/label=DNA:\BioBrick\suffix stem_loop 1999..2070 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 2007..2041 /vntifkey="39" /label=Stem\loop primer_bind complement(2134..2153) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(2238..2852) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 2253..2253 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 3019..3124 /vntifkey="39" /label=T0\terminator CDS complement(3137..3796) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 3910..3929 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(3986..3989) /vntifkey="39" /label=end\of\terminator stem_loop complement(3999..4018) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..1977 /vntifkey="21" /label=BBa_K523015 promoter 1..600 /vntifkey="30" /label=lac\promoter\and\lacZ RBS 609..618 /vntifkey="32" /label=strong\synthetic\E.\coli\ribosome\binding\site\with\SacI\site. misc_feature 625..1977 /vntifkey="21" /label=cenA\coding\sequence\encoding\Cellulomonas\fimi\endoglucanase\A BASE COUNT 933 a 1213 c 1088 g 813 t ORIGIN 1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac 61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt 121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc 181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag 361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg 421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga 601 tactagagct caaggaggta ctagatgtcc acccgcagaa ccgccgcagc gctgctggcg 661 gccgcggccg tcgccgtcgg cggtctgacc gccctcacca ccaccgccgc gcaggcggct 721 cccggctgcc gcgtcgacta cgccgtcacc aaccagtggc ccggcggctt cggcgccaac 781 gtcacgatca ccaacctcgg cgaccccgtc tcgtcgtgga agctcgactg gacctacacc 841 gcaggccagc ggatccagca gctgtggaac ggcaccgcgt cgaccaacgg cggccaggtc 901 tccgtcacca gcctgccctg gaacggcagc atcccgaccg gcggcacggc gtcgttcggg 961 ttcaacggct cgtgggccgg gtccaacccg acgccggcgt cgttctcgct caacggcacc 1021 acctgcacgg gcaccgtgcc gacgaccagc cccacgccga ccccgacgcc gacgaccccc 1081 acgccgacgc cgaccccgac ccccaccccc acgccgacgg tcacgccgca gccgaccagc 1141 ggcttctacg tcgacccgac gacgcagggc taccgcgcgt ggcaggccgc gtccggcacg 1201 gacaaggcgc tgctcgagaa gatcgcgctc accccgcagg cgtactgggt cggcaactgg 1261 gccgacgcgt cgcacgcgca ggccgaggtc gccgactaca ccggccgcgc cgtcgcggcc 1321 gggaagacgc cgatgctcgt cgtctacgcg atcccgggcc gcgactgcgg ctcgcactcc 1381 ggcggtggtg tgtccgagtc cgagtacgcg cgctgggtcg acaccgtcgc gcagggcatc 1441 aagggcaacc cgatcgtgat cctcgagccc gacgcgctcg cgcagctcgg cgactgctcc 1501 ggccagggtg accgcgtcgg cttcctcaag tacgccgcca agtcgctcac cctcaagggc 1561 gcgcgcgtct acatcgacgc gggccacgcg aagtggctgt cggtcgacac gccggtgaac 1621 cgcctcaacc aggtcggctt cgagtacgcg gtgggcttcg cgctcaacac gtcgaactac 1681 cagacgacgg cggacagcaa ggcgtacggc cagcagatct cgcagcggct gggcggcaag 1741 aagttcgtca tcgacacctc gcgcaacggc aacggctcga acggcgagtg gtgcaacccg 1801 cgcggccgcg cgctcggcga acgcccggtc gcggtgaacg acggctccgg cctggacgcg 1861 ctcctgtggg tcaagctgcc cggcgagtcc gacggcgcgt gcaacggcgg cccggccgcc 1921 ggccagtggt ggcaggagat cgccctggag atggcgcgca acgccaggtg gtaataatac 1981 tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 2041 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 2101 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 2161 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 2221 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 2281 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 2341 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 2401 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 2461 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 2521 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 2581 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 2641 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 2701 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 2761 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 2821 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 2881 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 2941 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 3001 gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt 3061 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag 3121 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat 3181 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg 3241 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga 3301 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg 3361 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac 3421 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc 3481 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat 3541 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca 3601 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct 3661 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact 3721 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag 3781 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata 3841 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca 3901 actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 3961 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat 4021 ttctggaatt cgcggccgct tctagag //