LOCUS pSB1C3 5199 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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#205=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -804 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 3130..3150 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 3151..3222 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 3159..3193 /vntifkey="39" /label=Stem\loop primer_bind complement(3286..3305) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(3390..4004) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 3405..3405 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 4171..4276 /vntifkey="39" /label=T0\terminator CDS complement(4289..4948) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 5062..5081 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(5138..5141) /vntifkey="39" /label=end\of\terminator stem_loop complement(5151..5170) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..3129 /vntifkey="21" /label=BBa_K525305 RBS 53..64 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency BASE COUNT 1385 a 1417 c 1189 g 1208 t ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga 61 gaaatactag agatggccgg cgctaccaag ctcgacaaga tgcgccagga gctgaaggca 121 gctgttgacg caaaggatct caagaaggct gaggaactgt accacaagat ctcctacgag 181 cttaagaccc gtactgttat cctcgaccgc gtctacggcc agtccacccg tgagctgctc 241 cgctccacct tcaaggcaga cgctcaggca ctgcgtgatt ctcttatcta cgacatcacc 301 gttgctatga aggcacgcga agctcaggac gcagttaagg ctggtaacct cgataaggca 361 aaggctgcac tggaccaggt caaccagtac gtttccaagg ttactgacgc tttcaaggca 421 gagctccaga aggctgcaca ggatgctaag gcagcttacg aggcagctct gccaccaaag 481 gtcgaatccg tgaccgcagt taacgctaag acccttgaga tcaagttcaa caaggcagtt 541 gacgctgcaa ccgtcatcga caacaagggc acttccgata cctctgacga cgttgttaag 601 gctaccgcaa tcaccctcaa ggctatcgat gaccaggtcc ctgtttccac tgttaaggca 661 tccctgtccg acgataagaa gaccctcaag ctggtcgtgg acggcgctca gttcttcacc 721 aagcgttacg ttgttgacat caagaacgtc aagacccttg atggtaagga cgttccagca 781 tacactacca ccatcgacac cactgattct gttcgcccat ccgtcctctc cttctcctac 841 gctgacaacg gcctgaccct caaggttaag ttctctgagc ctctggcatc cgttggcacc 901 gtcaagcttt acgacggtac cactgaaatc tccgtgtccc caaagttcac cgctggcgat 961 gacgagatga ccatcaacct cgcatcttcc tccgttccag ttaacaagga cctgaccctc 1021 aagatcttcg gcgctgtcga ttacaacggt aacgttatca accctaaccc agcagagctg 1081 actgttaaga agaccaccgt cgacatcacc aagccagttg ttcagtccat cgaagctgtc 1141 aacactaaga ccgtgaaggt taccttctct gagaagcttc tctccgcacc taccatcaag 1201 atcggcggcc agactgcttc cgtttccgtc gactctactg gtctggttta caccgcaacc 1261 ctcgcttccg cactgtccaa gggcgtttac gctgtcgagg tttccgatta caaggacctt 1321 gcaggcaact ctggtgacgc ttacactaag gttgtccagc tcaaggcaga taacaccgct 1381 ccaaagttcg tgtcctccca ggttgttaag atcaacggcg tcgaacacct ggttctcacc 1441 ttcgacgagg aggttaccac tggctccaac atcaccgtcg ttcagtcttc cgacaagtac 1501 atcgatgaaa acaacgttct gaaggcagtc ggtgctgacc ttaagaccac ctccgacaac 1561 ttcaagctct acctgccaac tgatggcaag tccaagtctg tggcactcaa catctcctcc 1621 ctgcctaagg gcacctacac cgttaccctt ccaaacggtc tcgtttccga cctggctgac 1681 aacccatacg cagagcgtaa gcagatcact ttcgtccgcg gctctgattc cctcaccacc 1741 aagcctgctc tggacaagga ctacgattcc aacggcgtta aggcagacaa caacaacgag 1801 cttgttttcg ctttcaccca gaacctcgac gcatccgctc tgaacctctc taacttcaac 1861 atcaacggtc tgactgtcac caaggcagtt ttcgatggcg acaccaagca catccgtgtt 1921 acccttgctc caggcgcaaa cacttggact ggtacccacg tcatcaccat ctccaacatc 1981 aagaacactt ccggcctcgt gatggacacc gttaccgtta acgaatacat gaaggagaac 2041 gtcgctccaa ccttcactgc aaccctgacc tccgctgatg ttatccgcgt tgacttctct 2101 gagcctgtcg caaacgctac catctcccgt gcactctccg ctaacaactt catcgttaag 2161 gttgacggca acgtcgtgac tgtttccaac gtttacgaag ataacaacgc aaccaacctg 2221 gtccagggtt ctaagggcta caagaccgtt taccttaagc tccagtcccc agttaccgac 2281 ctgtccaagc caatcactct ctccgctacc gacatcgtcg atgttaacca gaccggcgca 2341 atcaccgaca acaacgttgt cggtaacaac gtgtctgcta ctgttgttaa cgtcgcaaag 2401 accggcgtga gcaaaggcga agaactgttt accggcgtgg tgccgattct ggtggaactg 2461 gatggtgatg tgaacggcca taaatttagc gtgagcggcg aaggcgaagg tgatgcgacc 2521 tatggcaaac tgaccctgaa atttatttgc accacgggta aactgccggt tccgtggccg 2581 accctggtga ccacctttgg ctatggcctg atgtgctttg cgcgttatcc ggatcatatg 2641 aaacagcacg atttctttaa aagcgccatg ccggaaggct atgtgcagga acgcaccatc 2701 ttttttaaag atgatggcaa ctataaaacc cgtgcggaag tgaaatttga aggcgatacc 2761 ctggtgaacc gtattgaact gaaaggcatc gatttcaaag aagatggcaa cattctgggc 2821 cataaactgg aatataacta caacagccat aacgtgtata tcatggcgga taaacagaaa 2881 aacggcatca aagtgaactt taaaatccgc cacaacattg aagatggcag cgtgcagctg 2941 gccgatcatt atcagcagaa caccccgatt ggtgatggcc cggtgctgct gccggataac 3001 cattatctga gctaccagag caaactgagc aaagatccga acgaaaaacg tgatcacatg 3061 gtgctgctgg aatttgtgac cgcagccggt attaccctgg gcatggatga actgtacaaa 3121 accggttaat actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc 3181 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac 3241 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 3301 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 3361 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc 3421 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 3481 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 3541 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 3601 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 3661 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 3721 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 3781 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 3841 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 3901 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 3961 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 4021 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 4081 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 4141 tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg 4201 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa 4261 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact 4321 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct 4381 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa 4441 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc 4501 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt 4561 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt 4621 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag 4681 ggtgaacact 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