LOCUS pSB1C3 5991 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 5991 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 5991 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 3942 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 5986 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 5971 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 1 3924 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 3922 3942 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 3943 4014 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 3951 3985 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 4078 4097 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 4182 4796 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 4197 4197 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 4963 5068 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 5081 5740 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 5854 5873 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 5930 5933 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 5943 5962 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_K525406" 21 0 0 1 3921 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 53 64 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) "mCerulean CFP" 21 0 0 3199 3912 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #18=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #19=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #20=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. 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COMMENT 0.082322 1) COMMENT #208=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #127# 255 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Ahl}I (3924)" "@N (@S)" 5 -12.7307 COMMENT -8.71165 0 -10 3.69651 0.799245 #207#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #209=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1014 100) COMMENT (CObjectList) (CObjectList)) COMMENT #210=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -805 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 3922..3942 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 3943..4014 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 3951..3985 /vntifkey="39" /label=Stem\loop primer_bind complement(4078..4097) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(4182..4796) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 4197..4197 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 4963..5068 /vntifkey="39" /label=T0\terminator CDS complement(5081..5740) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 5854..5873 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(5930..5933) /vntifkey="39" /label=end\of\terminator stem_loop complement(5943..5962) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..3921 /vntifkey="21" /label=BBa_K525406 RBS 53..64 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency misc_feature 3199..3912 /vntifkey="21" /label=mCerulean\CFP BASE COUNT 1585 a 1677 c 1403 g 1326 t ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcctactag agaaagagga 61 gaaatactag agatggccgg cgctcaggtt aacgactaca acaagatctc cggctacgca 121 aaggaggctg ttcagtccct cgtcgaccag ggtgttatcc agggcgatac caacggcaac 181 ttcaacccac tgaacaccgt tactcgcgca caggctgctg agatcttcac caaggctctc 241 gaactggagg cagacggtga cgtcaacttc tccgatgtta agaagggcgc ttggtactac 301 aactctatcg cagctgttgt cgcaaacggc atcttcgagg gtgtgtccgc taacgagttc 361 gcaccaaaca agtcccttac ccgttccgag gctgcaaagg ttctcgttga cgctttcggc 421 ctggagggct ctgaatccct ctcccagttc gcagacgctt cccaggtcaa gggttgggca 481 aagtctgctc tggagaccgc agttgctaac ggcatcttca ctggctccga ggaaaacggt 541 aagcttaacc tcaagcctaa cgcagctatc acccgccagg atttcgcagt tgtcttcgct 601 cgtaccctgg acctcgcagt tgagaccgtt gacgcttccg tcaaggcaat caacaacact 661 accgtggagg ttaccttcga tgaagaggtt gacaacgtcg aggctctgaa gttcaagatc 721 gaaggccttg aggttaagaa cgcagctgtt aagcagacca acaagaaggt cgttgttctc 781 actaccgagg cacagaccgc tgacaaggaa tacgtcgtga ccctggatgg cgaggagatc 841 ggtaagttca agggcatcga agcagttgtt ccaaagtcca tcgtcctcaa gactaccaac 901 acccagggca aggttggtaa ccaggttacc ctgactgctg acgtcggcgt taaggcagct 961 ggcatcccag ttaccttcaa cgtcgacgca cctaccggct ctcttaacaa ggatgctgtg 1021 gttgaggttt acaccaacgc agagggcatc gcttcctact cctacactca gtacgcacca 1081 gacgctgacg atgtcaccgt ttacccaacc ggcgcacctc agctccgcgc tttcggtcca 1141 gtttactggg gcgtcgacaa catcctgacc atcgaagagg ttactaccgg caacaccctc 1201 gcaaacggtg ttaagaagac ctacaaggtc actttcaagg acccaaagac cggcgctgca 1261 ctgaccaaca agaagcttaa cgtgtccttc gttgagaaca ccaacgttgc tttcaacgca 1321 atctctaagg ctactgtcac caacccttcc tccggcctca ccgttacccc ataccagact 1381 accaccggcc tccaggaaga gatccaggtt accactgatt ccaacggcca ggcaaccttc 1441 gtcgtttctg gcaccaacac cgctgttact ccatacgtct tcgtggacgg ttcctcctcc 1501 gttctcggcg tttctaccgt caccggcacc aacaacgtta ctcaggcaac caccgctaac 1561 aagaagtggg aggcaaccga actgactgct accgcagctc aggttaagtt cgagggtgca 1621 cagcttaacc accagatcac cgtcgagcgt gacggcgaag aggaggcagc agctcagtac 1681 ggctccaagc tcaacggtcg cgaatacaag gttaaggttc tggataagga cggcaagcct 1741 tacgcaaacg gcctcgtcaa catcggtctg gacgaggtgc ttgatcgtaa cctcaacacc 1801 aactccaagg ctcagttcgc aaacgttact gacggcaccg ctctgaccct caacccaggc 1861 acctcccagc agggtaagat caagctgaac tctaagggcg aggcaactgt tgtcctttac 1921 ggcgctaagg gtgaagttgg caccccagtt gtctggatcg accagaacac ctcccagaac 1981 aaccagtccg gcgttctcga ggatggtgag cctttcttca aggcaccagt ttccaacttc 2041 cagtacgaac gcgtcatggg cgctgagttc accgtggagg gcaagacttc taaccagaac 2101 gttggtaccg acggcatcgc aaactacgct ttcgcactga ccaaccagtc cggcaagacc 2161 ctcactaacc tgatcggtaa gatgacctac gaaatccgta acacctccgg ccagccaatc 2221 gaggttaaga cccctatctc ctaccgcgac cagaacaacg tctcttccac tcttttcaag 2281 atcgaggaat acggctccat caccatctcc gctgatgtta tctctacctc caacgcagct 2341 ttcaccatcg agactgcacc aaacggtaag gctgcaaccg ttgtcgttac cccatccttc 2401 gttaccgcta acaacttcga cgcatccgct gacaacaacc tcaacggcgt cgtgactgat 2461 gcacgtgact tcaacaagca cgttaccgct cctgcactga ccgctacctt cggcaacaac 2521 aaggaggttg gtactaacta caccggcacc gtccagtact tcaacaagga caagaagacc 2581 atcactttca acggcaagaa cgcagttaag tacgctggtg aatctggcaa gacctacaag 2641 tacttcggcc tcggttccac cccaatctcc accgcagatg acttcatcac tcagctccag 2701 aacaacgctg caaacggctc cgttaccgtc acctacaagg ttgttgacaa cgaggtccag 2761 ttctacatcg tgtctttcaa caacgatggc gctgcaaacg ctgttaagcc agagcctacc 2821 gcagttgctc caactgacgg taccgtcgca ttcaccaaca cctccttcaa cgcttccgca 2881 aaccagacta tcaccgttac cgacgctgat cttaacgcag acgctaccgt tgcagaaact 2941 accaccgtca aggttacctc cggcactgtt gtctacaccc tcaccgctac cgagactggc 3001 gtgaacaccg gcgttttcac cgcaaccctg actgctgcac agctcgctgt tctgtctgac 3061 ggcgtcatca ccgcaaccta cgaggatgtt aagaacacca ctggctcctc cgttacccgc 3121 accgctaccg caactcttaa caccgtcgtt ggtcaggtta actacaacgc agcagctacc 3181 accgcatact ccaccggcgt gagcaaaggc gaagaactgt ttaccggcgt ggtgccgatt 3241 ctggtggaac tggatggtga tgtgaacggc cataaattta gcgtgagcgg cgaaggcgaa 3301 ggtgatgcga cctatggcaa actgaccctg aaattcattt gcaccaccgg caaactgccg 3361 gttccgtggc cgaccctggt taccaccctg acctggggcg tgcagtgctt tgcgcgttat 3421 ccggatcata tgaaacagca cgatttcttt aaaagcgcca tgccggaagg ctatgtgcag 3481 gaacgcacca tcttttttaa agatgatggc aactataaaa cccgtgcgga agtgaaattt 3541 gaaggcgata ccctggtgaa ccgtattgaa ctgaaaggca tcgatttcaa agaagatggc 3601 aacattctgg gccataaact ggaatataac tacatcagcg ataacgtgta tatcaccgcg 3661 gataaacaga aaaacggcat caaagcgaac tttaaaatcc gccacaacat tgaagatggc 3721 agcgtgcagc tggccgatca ttatcagcag aacaccccga ttggtgatgg cccggtgctg 3781 ctgccggata accattatct gagcacccag agcaaactga gcaaagatcc gaacgaaaaa 3841 cgtgatcaca tggtgctgct ggaatttgtg accgcagccg gtattaccct gggcatggat 3901 gaactgtaca aaaccggtta atactagtag cggccgctgc agtccggcaa aaaagggcaa 3961 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc 4021 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 4081 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 4141 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 4201 caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 4261 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 4321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 4381 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 4441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4501 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4561 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4621 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 4681 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 4741 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 4801 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 4861 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 4921 ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa 4981 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac 5041 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact 5101 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg 5161 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc 5221 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg 5281 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga 5341 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc 5401 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa 5461 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac 5521 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact 5581 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt 5641 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg 5701 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg 5761 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt 5821 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt 5881 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa 5941 tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga g //