LOCUS pSB1C3 3048 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 3048 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 3048 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 999 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3043 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3028 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 1 981 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 979 999 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 1000 1071 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 1008 1042 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 1135 1154 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 1239 1853 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 1254 1254 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 2020 2125 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 2138 2797 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 2911 2930 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 2987 2990 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 3000 3019 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_K542003" 21 0 0 1 978 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "Pbad promoter" 29 0 0 1 130 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 139 150 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #18=(CFSignal (CObList) COMMENT "tetracycline repressor from transposon Tn10 (+LVA)" 21 0 0 157 841 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #19=(CFSignal (CObList) "T1 from E. coli rrnB" 43 0 0 850 929 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #20=(CFSignal (CObList) "TE from coliphageT7" 43 0 0 938 978 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #21=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #22=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #23=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" COMMENT 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Creation Date: ? (no data)" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modification Date: ? (no data)" 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 3048 bp" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #32=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Associated Genes: rep (pMB1); ORI; Chloramphenicol resistance" COMMENT 1))) "Standard Fields") COMMENT #35=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #36=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #37=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #38=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #39=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2138 (3 #11# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #40=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2138 End: 2797 (Complementary)" COMMENT 1) COMMENT #41=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #42=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1160..1819)" 1) COMMENT #43=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #44=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"Chloramphenicol resistance\"" COMMENT 1))) "Chloramphenicol resistance"))) COMMENT "CDS (1 total)") COMMENT #45=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #46=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #47=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 978 " 1))) COMMENT "BBa_K542003") COMMENT #48=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 157 (3 #18# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #49=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 157 End: 841 " 1))) COMMENT "tetracycline repressor from transposon Tn10 (+LVA)") COMMENT #50=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 979 (3 #4# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #51=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 979 End: 999 " 1) COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #53=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1..21" 1))) "DNA: BioBrick suffix") COMMENT #54=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1239 (3 #8# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1239 End: 1853 (Complementary)" COMMENT 1) COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(261..875)" 1) COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"rep (pMB1)\"" 1))) COMMENT "rep (pMB1)") COMMENT #60=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1254 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #61=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1254 End: 1254" 1) COMMENT #62=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 276..276" 1) COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"ORI\"" 1))) "ORI"))) COMMENT "Misc. Feature (5 total)") COMMENT #66=(CFolderPar COMMENT (CGroupPar (CParagraph 28 (7 28 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #67=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1135 (3 #7# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1135 End: 1154 (Complementary)" COMMENT 1) COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #70=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(157..176)" 1))) COMMENT "Verification reverse (VR) primer binding site") COMMENT #71=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2911 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2911 End: 2930" 1) COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #74=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1933..1952" 1))) COMMENT "Verification forward (VF2) primer binding site"))) COMMENT "Primer Binding Site (2 total)") COMMENT #75=(CFolderPar COMMENT (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #76=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #77=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 130 " 1))) COMMENT "Pbad promoter"))) COMMENT "Promoter Eukaryotic (1 total)") COMMENT #78=(CFolderPar COMMENT (CGroupPar (CParagraph 32 (7 32 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #79=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 139 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 139 End: 150 " 1))) COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency"))) COMMENT "RBS (1 total)") COMMENT #81=(CFolderPar COMMENT (CGroupPar (CParagraph 39 (7 39 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #82=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1000 (3 #5# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1000 End: 1071" 1) COMMENT #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #85=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 22..93" 1))) COMMENT "E. coli his operon terminator") COMMENT #86=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1008 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1008 End: 1042" 1) COMMENT #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #89=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 30..64" 1))) "Stem loop") COMMENT #90=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2020 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #91=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2020 End: 2125" 1) COMMENT #92=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #93=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1042..1147" 1))) "T0 terminator") COMMENT #94=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2987 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2987 End: 2990 (Complementary)" COMMENT 1) COMMENT #96=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #97=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2009..2012)" 1))) COMMENT "end of terminator") COMMENT #98=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3000 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #99=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3000 End: 3019 (Complementary)" COMMENT 1) COMMENT #100=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #101=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2022..2041)" 1))) COMMENT "Stem loop"))) "Stem Loop (5 total)") COMMENT #102=(CFolderPar COMMENT (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #103=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 850 (3 #19# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #104=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 850 End: 929 " 1))) COMMENT "T1 from E. coli rrnB") COMMENT #105=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 938 (3 #20# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #106=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 938 End: 978 " 1))) COMMENT "TE from coliphageT7"))) COMMENT "Terminator (2 total)"))) "Feature Map") COMMENT #107=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #108=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #109=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #110=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 0 End: 0 " 1) COMMENT #111=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #112=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 0..0" 1))) COMMENT "DNA: BioBrick prefix"))) COMMENT "Misc. Feature (1 total)"))) COMMENT "Imported Features Not Shown on Map") COMMENT #113=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #114=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39120264 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #115=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #116=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 981 " 1))))) "AhlI: 1 site") 1 COMMENT 5 "ACTAGT" "TGATCA") COMMENT #117=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39549232 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #118=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #119=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 3028 " 1))))) "EcoRI: 1 site") COMMENT 1 5 "GAATTC" "CTTAAG") COMMENT #120=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39825784 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #121=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #122=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 999 " 1))))) "PstI: 1 site") 5 COMMENT 1 "CTGCAG" "GACGTC") COMMENT #123=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39825864 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #124=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #125=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 3043 " 1))))) "XbaI: 1 site") COMMENT 1 5 "TCTAGA" "AGATCT"))) COMMENT "Restriction/Methylation Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #126=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #127=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #128=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #129=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #130=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #131=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #132=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #133=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #134=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #135=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #136=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #137=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #138=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #139=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #140=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #141=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #142=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #143=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #144=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #145=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #146=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #147=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #148=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #149=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #150=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #151=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #152=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #153=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #154=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #155=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #156=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #157=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #158=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #159=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #160=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #161=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #162=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #163=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #164=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #165=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 COMMENT -2070) 1 1 0 1 1 COMMENT (mapper: 29.8822 -17.8387 40.0378 40.0378 0.01 10 -1 3048 3048 1 0 0) COMMENT #166=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -1148 100) COMMENT (CObjectList COMMENT #167=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1079 100) COMMENT (CObjectList COMMENT #168=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #132# 40177492 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27624 6.27424 1 0.0214037) 0.205813) COMMENT #169=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #129# 39076872 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "pSB1C3" "@N" 1 0 0.871165 0 -10 COMMENT 4.49575 1.39868 Nil) COMMENT #170=(CLabel COMMENT (CWidget 1002 (0 0) 1 2 0 0 #128# 32897328 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "3048 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 2.69745 0.799245 Nil)) (CObjectList)) COMMENT #171=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -874 100) COMMENT (CObjectList COMMENT #172=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -1153 100) COMMENT (CObjectList COMMENT #173=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #130# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 0.516591 1.87496 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #174=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Chloramphenicol resistance" "@N" 1 -17.3974 COMMENT 5.22699 0 -10 12.2634 1.04901 #173#)) COMMENT (CObjectList)) COMMENT #175=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil -246 100) COMMENT (CObjectList COMMENT #176=(CScratch COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #135# 32637208 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 4.26033 1.9 COMMENT 0.082322 1) COMMENT #177=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "DNA: BioBrick suffix" "@N" 1 15.3649 COMMENT -5.22699 0 -10 8.81667 1.04901 #176#) COMMENT #178=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #8# 0) 1 2 0 0 #131# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.45947 3.72522 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #179=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "rep (pMB1)" COMMENT "@N" 1 -3.75121 -12.1963 0 -10 4.77049 COMMENT 1.04901 #178#) COMMENT #180=(CScratch COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #135# 39223840 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 3.69435 1.9 COMMENT 0.082322 1) COMMENT #181=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #127# 268697599 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "ORI" "@N" 1 COMMENT 8.09048 -15.681 0 -10 1.67342 1.04901 #180#) COMMENT #182=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #131# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 4.26033 COMMENT 6.27319 1 0.082322) 0.8 1.8 0) COMMENT #183=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #127# -1073741824 COMMENT 100) (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "BBa K542003" COMMENT "@N" 1 13.3495 6.96932 0 -10 5.91941 1.04901 COMMENT #182#) COMMENT #184=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #131# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 4.5423 5.95212 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #185=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #127# 2030746241 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "tetracycline repressor from transposon Tn10 (+LVA)" COMMENT "@N" 1 22.16 5.22699 0 -10 22.3039 1.04901 COMMENT #184#)) (CObjectList)) COMMENT #186=(CGroupWidget COMMENT (CWidget 28 (7 28 0) 1 2 0 0 Nil -944 100) COMMENT (CObjectList COMMENT #187=(CScratch COMMENT (CWidget 0 (3 #7# 0) 1 2 0 0 #148# 39560720 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 3.93926 1.9 COMMENT 0.082322 1) COMMENT #188=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification reverse (VR) primer binding site" COMMENT "@N" 1 18.8616 -13.9386 0 -10 19.1319 1.04901 COMMENT #187#) COMMENT #189=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #148# 38926160 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.284022 1.9 COMMENT 0.082322 1) COMMENT #190=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification forward (VF2) primer binding site" COMMENT "@N" 1 -18.3804 8.71165 0 -10 19.4816 1.04901 COMMENT #189#)) (CObjectList)) COMMENT #191=(CGroupWidget COMMENT (CWidget 29 (7 29 0) 1 2 0 0 Nil -1223 100) COMMENT (CObjectList COMMENT #192=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #131# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 6.00563 6.27319 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #193=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Pbad promoter" COMMENT "@N" 1 5.74003 12.1963 0 -10 6.44391 1.04901 COMMENT #192#)) (CObjectList)) COMMENT #194=(CGroupWidget COMMENT (CWidget 32 (7 32 0) 1 2 0 0 Nil -316 100) COMMENT (CObjectList COMMENT #195=(CScratch COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #147# 39186312 COMMENT 100) (LOGPEN 0 6 16711680) 8 1 5.98916 1.9 COMMENT 0.082322 1) COMMENT #196=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #127# 268632496 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT "@N" 1 15.7863 10.454 0 -10 19.032 1.04901 COMMENT #195#)) (CObjectList)) COMMENT #197=(CGroupWidget COMMENT (CWidget 39 (7 39 0) 1 2 0 0 Nil -316 100) COMMENT (CObjectList COMMENT #198=(CLine COMMENT (CWidget 0 (3 #5# 0) 1 2 0 0 #148# 1210 100) COMMENT (LOGPEN 0 0 16711680) 1 0.835356 4.06893 COMMENT 4.21711) COMMENT #199=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #127# 858 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "E. coli his operon terminator" "@N" 1 COMMENT 17.0028 -12.1963 0 -10 12.3383 1.04901 #198#) COMMENT #200=(CLine COMMENT (CWidget 0 (3 #6# 0) 1 2 0 0 #148# 1210 100) COMMENT (LOGPEN 0 0 16711680) 1 0.670712 4.12861 COMMENT 4.20065) COMMENT #201=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #127# 806 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Stem loop" "@N" COMMENT 1 13.0676 -10.454 0 -10 4.42082 1.04901 #200#) COMMENT #202=(CLine COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #148# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 0.835356 1.89966 COMMENT 2.11782) COMMENT #203=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 8 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T0 terminator" COMMENT "@N" 1 -13.9982 -5.22699 0 -10 5.74457 COMMENT 1.04901 #202#) COMMENT #204=(CScratch COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #148# 39125648 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.127604 1.9 COMMENT 0.082322 1) COMMENT #205=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "end of terminator" "@N" 1 -10.2544 10.454 0 COMMENT -10 7.41799 1.04901 #204#) COMMENT #206=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #148# 39177064 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.100848 1.9 COMMENT 0.082322 1) COMMENT #207=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Stem loop" "@N" COMMENT 1 -4.18439 12.1963 0 -10 4.42082 1.04901 COMMENT #206#)) (CObjectList)) COMMENT #208=(CGroupWidget COMMENT (CWidget 43 (7 43 0) 1 2 0 0 Nil -246 100) COMMENT (CObjectList COMMENT #209=(CLine COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #135# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 0.670712 4.36118 COMMENT 4.52583) COMMENT #210=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #127# 268632356 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "T1 from E. coli rrnB" "@N" 1 16.0725 COMMENT -1.74233 0 -10 8.39207 1.04901 #209#) COMMENT #211=(CLine COMMENT (CWidget 0 (3 #20# 0) 1 2 0 0 #135# 1210 100) COMMENT (LOGPEN 0 8 13395507) 10 0.670712 4.26033 COMMENT 4.34472) COMMENT #212=(CLabel (CWidget 0 (0 0) 1 2 0 0 #127# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "TE from coliphageT7" "@N" 1 16.0327 -3.48466 COMMENT 0 -10 8.81667 1.04901 #211#)) (CObjectList))) COMMENT (CObjectList)) COMMENT #213=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -386 100) COMMENT (CObjectList COMMENT #214=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -1293 100) COMMENT (CObjectList COMMENT #215=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #134# 38952040 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 4.21917 1.9 COMMENT 0.082322 1) COMMENT #216=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #136# 2030746125 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Pst}I (999)" "@N (@S)" 5 13.019 COMMENT -8.71165 0 -10 3.37181 0.799245 #215#)) COMMENT (CObjectList)) COMMENT #217=(CGroupWidget COMMENT (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -525 100) COMMENT (CObjectList COMMENT #218=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #134# 39190016 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.0123488 1.9 COMMENT 0.082322 1) COMMENT #219=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #136# 1628250141 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Xba}I (3043)" "@N (@S)" 5 -3.56237 COMMENT 15.681 0 -10 3.97125 0.799245 #218#)) COMMENT (CObjectList)) COMMENT #220=(CGroupWidget COMMENT (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -456 100) COMMENT (CObjectList COMMENT #221=(CScratch COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #134# 39179248 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.0432208 1.9 COMMENT 0.082322 1) COMMENT #222=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #136# 32897552 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Eco}RI (3028)" "@N (@S)" 5 -4.05614 COMMENT 13.9386 0 -10 4.32092 0.799245 #221#)) COMMENT (CObjectList)) COMMENT #223=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -1363 100) COMMENT (CObjectList COMMENT #224=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #134# 32612696 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 4.25622 1.9 COMMENT 0.082322 1) COMMENT #225=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #136# 39077352 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Ahl}I (981)" "@N (@S)" 5 13.1344 COMMENT -6.96932 0 -10 3.27191 0.799245 #224#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #226=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1223 100) COMMENT (CObjectList) (CObjectList)) COMMENT #227=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -944 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 979..999 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 1000..1071 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 1008..1042 /vntifkey="39" /label=Stem\loop primer_bind complement(1135..1154) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1239..1853) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1254..1254 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 2020..2125 /vntifkey="39" /label=T0\terminator CDS complement(2138..2797) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2911..2930 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2987..2990) /vntifkey="39" /label=end\of\terminator stem_loop complement(3000..3019) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..978 /vntifkey="21" /label=BBa_K542003 promoter 1..130 /vntifkey="29" /label=Pbad\promoter RBS 139..150 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency misc_feature 157..841 /vntifkey="21" /label=tetracycline\repressor\from\transposon\Tn10\(+LVA) terminator 850..929 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 938..978 /vntifkey="43" /label=TE\from\coliphageT7 BASE COUNT 863 a 702 c 688 g 795 t ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa 181 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt 241 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag 301 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc 361 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt 421 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa 481 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag 541 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat 601 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca 661 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc 721 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct 781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt 1021 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc 1081 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 1141 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 1201 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag 1261 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 1321 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 1381 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 1441 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 1501 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 1561 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 1621 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 1681 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 1741 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 1801 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 1861 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 1921 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 1981 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa 2041 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg 2101 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat 2161 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat 2221 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca 2281 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga 2341 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat 2401 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga 2461 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg 2521 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa 2581 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt 2641 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat 2701 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata 2761 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa 2821 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg 2881 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc 2941 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc 3001 ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagag //