LOCUS pSB1C3 3866 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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/vntifkey="39" /label=Stem\loop primer_bind complement(1953..1972) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(2057..2671) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 2072..2072 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 2838..2943 /vntifkey="39" /label=T0\terminator CDS complement(2956..3615) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 3729..3748 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(3805..3808) /vntifkey="39" /label=end\of\terminator stem_loop complement(3818..3837) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..1796 /vntifkey="21" /label=BBa_K592016 RBS 1..12 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency misc_feature 19..1149 /vntifkey="21" /label=YF1\blue-light\sensor\protein RBS 1158..1169 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency misc_feature 1176..1796 /vntifkey="21" /label=FixJ,\the\response\regulator\to\YF1 BASE COUNT 982 a 1045 c 1001 g 838 t ORIGIN 1 aaagaggaga aatactagat ggctagtttt caatcatttg ggataccagg acagctggaa 61 gtcatcaaaa aagcacttga tcacgtgcga gtcggtgtgg taattacaga tcccgcactt 121 gaagataatc ctattgtcta cgtaaatcaa ggctttgttc aaatgaccgg ctacgagacc 181 gaggaaattt taggaaagaa ctgtcgcttc ttacagggga aacacacaga tccggcagaa 241 gtggacaaca tcagaaccgc tttacaaaat aaagaaccgg tcaccgttca gatccaaaac 301 tacaaaaaag acggaacgat gttctggaat gaattaaata ttgatccaat ggaaatagag 361 gataaaacgt attttgtcgg cattcagaat gatatcaccg agcaccagca gacccaggcg 421 cgtctccagg aactgcaatc cgagctcgtc cacgtctcca ggctgagcgc catgggcgaa 481 atggcgtccg cgctcgcgca cgagctcaac cagccgctgg cggcgatcag caactacatg 541 aagggctcgc ggcggctgct tgccggcagc agtgatccga acacaccgaa ggtcgaaagc 601 gccctggacc gcgccgccga gcaggcgctg cgcgccggcc agatcatccg gcgcctgcgc 661 gacttcgttg cccgcggcga atcggagaag cgggtcgaga gtctctccaa gctgatcgag 721 gaggccggcg cgctcgggct tgccggcgcg cgcgagcaga acgtgcagct ccgcttcagt 781 ctcgatccgg gcgccgatct cgttctcgcc gaccgggtgc agatccagca ggtcctggtc 841 aacctgttcc gcaacgcgct ggaagcgatg gctcagtcgc agcgacgcga gctcgtcgtc 901 accaacaccc ccgccgccga cgacatgatc gaggtcgaag tgtccgacac cggcagcggt 961 ttccaggacg acgtcattcc gaacctgttt cagactttct tcaccaccaa ggacaccggc 1021 atgggcgtgg gactgtccat cagccgctcg atcatcgaag ctcacggcgg gcgcatgtgg 1081 gccgagagca acgcatcggg cggggcgacc ttccgcttca ccctcccggc agccgacgag 1141 aattaataat actagagaaa gaggagaaat actagatgac gaccaaggga catatctacg 1201 tcatcgacga cgacgcggcg atgcgggatt cgctgaattt cctgctggat tctgccggct 1261 tcggcgtcac gctgtttgac gacgcgcaag cctttctcga cgccctgccg ggtctctcct 1321 tcggctgcgt cgtctccgac gtgcgcatgc cgggccttga cggcatcgag ctgttgaagc 1381 ggatgaaggc gcagcaaagc ccctttccga tcctcatcat gaccggtcac ggcgacgtgc 1441 cgctcgcggt cgaggcgatg aagttagggg cggtcgactt tctggaaaag cctttcgagg 1501 acgaccgcct caccgccatg atcgaatcgg cgatccgcca ggccgagccg gccgccaaga 1561 gcgaggccgt cgcgcaggat atcgccgccc gcgtcgcctc gttgagcccc agggagcgcc 1621 aggtcatgga agggctgatc gccggccttt ccaacaagct gatcgcccgc gagtacgaca 1681 tcagcccgcg caccatcgag gtgtatcggg ccaacgtcat gaccaagatg caggccaaca 1741 gcctttcgga gctggttcgc ctcgcgatgc gcgccggcat gctcaacgat taataatact 1801 agtagcggcc gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct 1861 ttaaaaccga aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc 1921 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 1981 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 2041 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat 2101 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 2161 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 2221 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 2281 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 2341 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 2401 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 2461 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 2521 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2581 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 2641 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 2701 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 2761 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 2821 tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt 2881 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc 2941 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt 3001 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg 3061 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa 3121 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga 3181 gacgaaaaac atattctcaa taaacccttt 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