LOCUS pSB1C3 4010 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 4010 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 4010 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1961 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 4005 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3990 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 1 1943 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 1941 1961 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 1962 2033 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 1970 2004 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 2097 2116 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 2201 2815 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 2216 2216 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 2982 3087 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 3100 3759 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 3873 3892 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 3949 3952 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 3962 3981 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_K592020" 21 0 0 1 1940 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "FixK2 promoter" 29 0 0 1 250 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 259 270 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #18=(CFSignal (CObList) "cI repressor from E. coli phage lambda (+LVA)" COMMENT 21 0 0 277 1051 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #19=(CFSignal (CObList) "T1 from E. coli rrnB" 43 0 0 1060 1139 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #20=(CFSignal (CObList) "TE from coliphage T7" 43 0 0 1148 1188 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #21=(CFSignal (CObList) "promoter (lambda cI regulated)" 29 0 0 1197 COMMENT 1245 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #22=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 1254 1265 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #23=(CFSignal (CObList) "amilCP, blue chromoprotein" 21 0 0 1272 1940 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #24=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #25=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #26=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" COMMENT 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Creation Date: ? (no data)" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modification Date: ? 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1941..1961 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 1962..2033 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 1970..2004 /vntifkey="39" /label=Stem\loop primer_bind complement(2097..2116) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(2201..2815) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 2216..2216 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 2982..3087 /vntifkey="39" /label=T0\terminator CDS complement(3100..3759) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 3873..3892 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(3949..3952) /vntifkey="39" /label=end\of\terminator stem_loop complement(3962..3981) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..1940 /vntifkey="21" /label=BBa_K592020 promoter 1..250 /vntifkey="29" /label=FixK2\promoter RBS 259..270 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency misc_feature 277..1051 /vntifkey="21" /label=cI\repressor\from\E.\coli\phage\lambda\(+LVA) terminator 1060..1139 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 1148..1188 /vntifkey="43" /label=TE\from\coliphage\T7 promoter 1197..1245 /vntifkey="29" /label=promoter\(lambda\cI\regulated) RBS 1254..1265 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency misc_feature 1272..1940 /vntifkey="21" /label=amilCP,\blue\chromoprotein BASE COUNT 1112 a 947 c 956 g 995 t ORIGIN 1 gccggagccg attatccgca cccgtccttg gtcttggaca cactcgctcg cgatccgaag 61 ccgcctttca aagatactcc gcagtgaaac gcatctttta agcgcgactt gtcgccttcg 121 cgcagcagaa cactcgtatg gcactcaatc catgatcgct tcgttcgctc cgacgcgcgt 181 tgcggggccc ccctcgaccg gatgacaaca cattgcgtca cctcaactgc tgcgcgcgca 241 ctgcgtcaca tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 301 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 361 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 421 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 481 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 541 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 601 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 661 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 721 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 781 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 841 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 901 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 961 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 1021 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 1081 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1141 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac 1201 accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgctacta gagaaagagg 1261 agaaatacta gatgagtgtg atcgctaaac aaatgaccta caaggtttat atgtcaggca 1321 cggtcaatgg acactacttt gaggtcgaag gcgatggaaa aggtaagccc tacgaggggg 1381 agcagacggt aaagctcact gtcaccaagg gcggacctct gccatttgct tgggatattt 1441 tatcaccaca gtgtcagtac ggaagcatac cattcaccaa gtaccctgaa gacatccctg 1501 actatgtaaa gcagtcattc ccggagggct atacatggga gaggatcatg aactttgaag 1561 atggtgcagt gtgtactgtc agcaatgatt ccagcatcca aggcaactgt ttcatctacc 1621 atgtcaagtt ctctggtttg aactttcctc ccaatggacc tgtcatgcag aagaagacac 1681 agggctggga acccaacact gagcgtctct ttgcacgaga tggaatgctg ctaggaaaca 1741 actttatggc tctgaagtta gaaggaggcg gtcactattt gtgtgaattt aaaactactt 1801 acaaggcaaa gaagcctgtg aagatgccag ggtatcacta tgttgaccgc aaactggatg 1861 taaccaatca caacaaggat tacacttcgg ttgagcagtg tgaaatttcc attgcacgca 1921 aacctgtggt cgcctaataa tactagtagc ggccgctgca gtccggcaaa aaagggcaag 1981 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct 2041 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 2101 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 2161 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac 2221 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 2281 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 2341 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 2401 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 2461 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 2521 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 2581 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 2641 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 2701 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 2761 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 2821 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 2881 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 2941 taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa 3001 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact 3061 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc 3121 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc 3181 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc 3241 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt 3301 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa 3361 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg 3421 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac 3481 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg 3541 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 3601 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 3661 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 3721 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 3781 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 3841 ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta 3901 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat 3961 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag //