LOCUS BBa_K59202 2793 bp DNA circular 20-AUG-2017 DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT http://www.invitrogen.com/ COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|-11904168| COMMENT VNTDBDATE|-11904170| COMMENT VNTNAME|BBa_K592024| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "BBa_K592024" 0 2793 COMMENT (CDBMol 0 0 1 1 1 0 0 1952540519 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" COMMENT "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 2793 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #0=(CRSite (CStringList) "ApaLI" "gtgcac" 2 0 2 94 0 1251 0 0 "") COMMENT #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 2 1757 0 2649 0 0 "") COMMENT #2=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 0 0 "") COMMENT #3=(CRSite (CStringList) "ClaI" "atcgat" 3 0 0 0 "") COMMENT #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2773 0 0 "") COMMENT #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 0 0 "") COMMENT #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 2024 0 0 "") COMMENT #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 744 0 0 "") COMMENT #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") COMMENT #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 0 0 "")) COMMENT (CObList COMMENT #10=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 724 744 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "724..744") COMMENT #11=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 745 816 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "745..816") COMMENT #12=(CFSignal (CObList) "Stem loop" 39 0 0 753 787 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "753..787") COMMENT #13=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 880 899 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(880..899)") COMMENT #14=(CFSignal (CObList) "rep (pMB1)" 21 0 1 984 1598 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 COMMENT "complement(984..1598)") COMMENT #15=(CFSignal (CObList) "ORI" 21 0 0 999 999 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "999..999") COMMENT #16=(CFSignal (CObList) "T0 terminator" 39 0 0 1765 1870 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1765..1870") COMMENT #17=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 1883 2542 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1883..2542)") COMMENT #18=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 2656 2675 0 (CStringList) (CStringList) 1 1 1 1 "2656..2675") COMMENT #19=(CFSignal (CObList) "end of terminator" 39 0 1 2732 2735 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2732..2735)") COMMENT #20=(CFSignal (CObList) "Stem loop" 39 0 1 2745 2764 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2745..2764)") COMMENT #21=(CFSignal (CObList) "DNA: BioBrick prefix" 21 0 0 2051 2072 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2051..2072") COMMENT #22=(CFSignal (CObList) "mTagBFP" 4 0 0 19 717 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "19..717") COMMENT #23=(CFSignal (CObList) "BBa_K592024" 21 0 0 1 723 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #24=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 1 12 0 (CStringList) (CStringList) 1 1 1 1 "1..12") COMMENT #25=(CFSignal (CObList) "Blue Fluorescent Protein (mTagBFP)" 21 0 0 19 COMMENT 723 0 (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #26=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #27=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "BBa_K592024" 2) 5 COMMENT "" 0 4) COMMENT #28=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'BBa_K592024'" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. 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(CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 10 2081718712 (CObList))) FEATURES Location/Qualifiers misc_feature 724..744 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 745..816 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 753..787 /vntifkey="39" /label=Stem\loop primer_bind complement(880..899) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(984..1598) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 999..999 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1765..1870 /vntifkey="39" /label=T0\terminator CDS complement(1883..2542) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2656..2675 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2732..2735) /vntifkey="39" /label=end\of\terminator stem_loop complement(2745..2764) /vntifkey="39" /label=Stem\loop misc_feature 2051..2072 /vntifkey="21" /label=DNA:\BioBrick\prefix RBS 1..12 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency CDS 19..717 /vntifkey="4" /label=mTagBFP /note="K592100" misc_feature 1..723 /vntifkey="21" /label=BBa_K592024 misc_feature 19..723 /vntifkey="21" /label=Blue\Fluorescent\Protein\(mTagBFP) BASE COUNT 769 a 672 c 676 g 676 t ORIGIN 1 aaagaggaga aatactagat gagcgaactg atcaaagaga acatgcacat gaagctgtac 61 atggaaggca ccgttgacaa ccaccacttt aagtgcacgt ctgagggtga gggtaagccg 121 tacgaaggca cccaaaccat gcgtatcaaa gttgtggagg gcggtccact gccgttcgct 181 tttgacattc tggcgaccag cttcctgtac ggttccaaaa cgttcattaa ccatactcag 241 ggcattccgg atttcttcaa acagagcttt ccggaaggtt tcacctggga gcgtgtcacc 301 acgtatgaag atggtggtgt gttgaccgcc acccaagata cctccctgca agatggctgt 361 ctgatctata acgtgaaaat tcgtggcgtc aactttacga gcaatggtcc ggtgatgcag 421 aagaaaaccc tgggttggga ggcgtttacg gaaaccctgt atccggccga tggtggcctg 481 gagggccgta acgacatggc actgaagctg gttggtggca gccatttgat cgcaaatatc 541 aagacgacgt accgcagcaa gaaaccggcg aaaaatctga agatgccggg tgtttactat 601 gtcgactacc gtctggaacg cattaaagaa gcgaataatg agacttacgt ggagcagcac 661 gaggttgcag tcgcgcgcta ttgcgacttg cctagcaagc tgggtcataa actgaattaa 721 taatactagt agcggccgct gcagtccggc aaaaaagggc aaggtgtcac caccctgccc 781 tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc 841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga 1021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 1081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 1141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 1201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 1261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 1321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 1381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 1441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 1501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 1561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 1621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 1681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 1741 aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc 1801 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag 1861 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta 1921 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg 1981 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg 2041 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat 2101 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac 2161 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt 2221 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa 2281 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat 2341 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta 2401 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa 2461 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat 2521 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa 2581 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc 2641 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 2701 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag 2761 gatgatttct ggaattcgcg gccgcttcta gag //