LOCUS pSB1C3 2125 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 2125 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 2125 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 76 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2120 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2105 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 1 58 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 56 76 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 77 148 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 85 119 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 212 231 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 316 930 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 331 331 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 1097 1202 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 1215 1874 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 1988 2007 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 2064 2067 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 2077 2096 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_K608002" 21 0 0 1 55 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "constitutive promoter family member" 30 0 0 1 COMMENT 35 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 44 55 0 (CStringList) (CStringList) 1 1 1 1 "")) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #18=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #19=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #20=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" COMMENT 1) COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Creation Date: ? (no data)" 1) COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modification Date: ? (no data)" 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 2125 bp" 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #29=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Associated Genes: rep (pMB1); ORI; Chloramphenicol resistance" COMMENT 1))) "Standard Fields") COMMENT #32=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #33=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #34=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #35=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #36=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1215 (3 #11# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #37=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1215 End: 1874 (Complementary)" COMMENT 1) COMMENT #38=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #39=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1160..1819)" 1) COMMENT #40=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #41=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"Chloramphenicol resistance\"" COMMENT 1))) "Chloramphenicol resistance"))) COMMENT "CDS (1 total)") COMMENT #42=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #43=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #44=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 55 " 1))) COMMENT "BBa_K608002") COMMENT #45=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 56 (3 #4# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #46=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 56 End: 76 " 1) COMMENT #47=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1..21" 1))) "DNA: BioBrick suffix") COMMENT #49=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 316 (3 #8# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #50=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 316 End: 930 (Complementary)" COMMENT 1) COMMENT #51=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(261..875)" 1) COMMENT #53=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"rep (pMB1)\"" 1))) COMMENT "rep (pMB1)") COMMENT #55=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 331 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 331 End: 331 " 1) COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 276..276" 1) COMMENT #59=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"ORI\"" 1))) "ORI"))) COMMENT "Misc. Feature (4 total)") COMMENT #61=(CFolderPar COMMENT (CGroupPar (CParagraph 28 (7 28 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #62=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 212 (3 #7# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 212 End: 231 (Complementary)" COMMENT 1) COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #65=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(157..176)" 1))) COMMENT "Verification reverse (VR) primer binding site") COMMENT #66=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1988 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1988 End: 2007" 1) COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1933..1952" 1))) COMMENT "Verification forward (VF2) primer binding site"))) COMMENT "Primer Binding Site (2 total)") COMMENT #70=(CFolderPar COMMENT (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #71=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 35 " 1))) COMMENT "constitutive promoter family member"))) COMMENT "Promoter Prokaryotic (1 total)") COMMENT #73=(CFolderPar COMMENT (CGroupPar (CParagraph 32 (7 32 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #74=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 44 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #75=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 44 End: 55 " 1))) COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency"))) COMMENT "RBS (1 total)") COMMENT #76=(CFolderPar COMMENT (CGroupPar (CParagraph 39 (7 39 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #77=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 77 (3 #5# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #78=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 77 End: 148 " 1) COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 22..93" 1))) COMMENT "E. coli his operon terminator") COMMENT #81=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 85 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #82=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 85 End: 119 " 1) COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 30..64" 1))) "Stem loop") COMMENT #85=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1097 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #86=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1097 End: 1202" 1) COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1042..1147" 1))) "T0 terminator") COMMENT #89=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2064 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #90=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2064 End: 2067 (Complementary)" COMMENT 1) COMMENT #91=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #92=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2009..2012)" 1))) COMMENT "end of terminator") COMMENT #93=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2077 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #94=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2077 End: 2096 (Complementary)" COMMENT 1) COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #96=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2022..2041)" 1))) COMMENT "Stem loop"))) "Stem Loop (5 total)"))) COMMENT "Feature Map") COMMENT #97=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #98=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #99=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #100=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 0 End: 0 " 1) COMMENT #101=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #102=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 0..0" 1))) "DNA: BioBrick prefix"))) COMMENT "Misc. Feature (1 total)"))) COMMENT "Imported Features Not Shown on Map") COMMENT #103=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #104=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39635016 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #105=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #106=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 58 " 1))))) "AhlI: 1 site") 1 COMMENT 5 "ACTAGT" "TGATCA") COMMENT #107=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39424248 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #108=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #109=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 2105 " 1))))) "EcoRI: 1 site") COMMENT 1 5 "GAATTC" "CTTAAG") COMMENT #110=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39424008 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #111=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #112=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 76 " 1))))) "PstI: 1 site") 5 COMMENT 1 "CTGCAG" "GACGTC") COMMENT #113=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 39424088 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #114=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #115=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 2120 " 1))))) "XbaI: 1 site") COMMENT 1 5 "TCTAGA" "AGATCT"))) COMMENT "Restriction/Methylation Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #116=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #117=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #118=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #119=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #120=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #121=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #122=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #123=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #124=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #125=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #126=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #127=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #128=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #129=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #130=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #131=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #132=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #133=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #134=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #135=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #136=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #137=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #138=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #139=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #140=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #141=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #142=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #143=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #144=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #145=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #146=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #147=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #148=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #149=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #150=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #151=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #152=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #153=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #154=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #155=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 COMMENT -2070) 1 1 0 1 1 COMMENT (mapper: 29.8822 -19.7119 40.0378 40.0378 0.01 10 -1 2125 2125 1 0 0) COMMENT #156=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -1148 100) COMMENT (CObjectList COMMENT #157=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -246 100) COMMENT (CObjectList COMMENT #158=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #122# 40809084 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27714 6.27514 1 0.0214037) 0.295209) COMMENT #159=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #119# 971674420 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "pSB1C3" "@N" 1 0 0.871165 0 -10 COMMENT 4.49575 1.39868 Nil) COMMENT #160=(CLabel COMMENT (CWidget 1002 (0 0) 1 2 0 0 #118# -858993459 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "2125 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 2.34778 0.799245 Nil)) (CObjectList)) COMMENT #161=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -177 100) COMMENT (CObjectList COMMENT #162=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -321 100) COMMENT (CObjectList COMMENT #163=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #120# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 0.740974 2.68935 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #164=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Chloramphenicol resistance" "@N" 1 -17.9222 COMMENT -1.74233 0 -10 12.2634 1.04901 #163#)) COMMENT (CObjectList)) COMMENT #165=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil -107 100) COMMENT (CObjectList COMMENT #166=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #121# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 6.04883 6.11082 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #167=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "DNA: BioBrick suffix" "@N" 1 10.8642 10.454 COMMENT 0 -10 8.81667 1.04901 #166#) COMMENT #168=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #8# 0) 1 2 0 0 #121# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 3.52774 5.34328 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #169=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "rep (pMB1)" COMMENT "@N" 1 14.004 -3.48466 0 -10 4.77049 1.04901 COMMENT #168#) COMMENT #170=(CScratch COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #125# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 1 5.299 1.9 0.082322 COMMENT 1) COMMENT #171=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "ORI" "@N" 1 COMMENT 12.6923 1.74233 0 -10 1.67342 1.04901 #170#) COMMENT #172=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #121# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 6.11082 6.27319 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #173=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "BBa K608002" COMMENT "@N" 1 4.89698 15.681 0 -10 5.91941 1.04901 COMMENT #172#)) (CObjectList)) COMMENT #174=(CGroupWidget COMMENT (CWidget 28 (7 28 0) 1 2 0 0 Nil -251 100) COMMENT (CObjectList COMMENT #175=(CLine COMMENT (CWidget 0 (3 #7# 0) 1 2 0 0 #138# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 0.835356 5.59125 COMMENT 5.65029) COMMENT #176=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification reverse (VR) primer binding site" COMMENT "@N" 1 20.9548 3.48466 0 -10 19.1319 1.04901 COMMENT #175#) COMMENT #177=(CLine COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #138# COMMENT 1605442005 100) (LOGPEN 0 0 16711680) 1 COMMENT 0.835356 0.348346 0.407388) COMMENT #178=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 1 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification forward (VF2) primer binding site" COMMENT "@N" 1 -18.3804 8.71165 0 -10 19.4816 1.04901 COMMENT #177#)) (CObjectList)) COMMENT #179=(CGroupWidget COMMENT (CWidget 30 (7 30 0) 1 2 0 0 Nil -1228 100) COMMENT (CObjectList COMMENT #180=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #133# 0 100) COMMENT (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 0.835356 COMMENT 6.16986 6.27319 1 0.082322) 0.8 1.8 0) COMMENT #181=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "constitutive promoter family member" "@N" 1 COMMENT 9.77333 17.4233 0 -10 15.9599 1.04901 #180#)) COMMENT (CObjectList)) COMMENT #182=(CGroupWidget COMMENT (CWidget 32 (7 32 0) 1 2 0 0 Nil -461 100) COMMENT (CObjectList COMMENT #183=(CScratch COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #137# 0 100) COMMENT (LOGPEN 0 6 16711680) 8 1 6.14625 1.9 COMMENT 0.082322 1) COMMENT #184=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT "@N" 1 11.8292 13.9386 0 -10 19.032 1.04901 COMMENT #183#)) (CObjectList)) COMMENT #185=(CGroupWidget COMMENT (CWidget 39 (7 39 0) 1 2 0 0 Nil -386 100) COMMENT (CObjectList COMMENT #186=(CLine COMMENT (CWidget 0 (3 #5# 0) 1 2 0 0 #138# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 0.835356 5.83628 COMMENT 6.04883) COMMENT #187=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "E. coli his operon terminator" "@N" 1 COMMENT 17.1247 5.22699 0 -10 12.3383 1.04901 #186#) COMMENT #188=(CLine COMMENT (CWidget 0 (3 #6# 0) 1 2 0 0 #138# 1095190356 COMMENT 100) (LOGPEN 0 0 16711680) 1 0.670712 COMMENT 5.92189 6.02521) COMMENT #189=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Stem loop" "@N" COMMENT 1 12.2877 6.96932 0 -10 4.42082 1.04901 #188#) COMMENT #190=(CLine COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #138# 1 100) COMMENT (LOGPEN 0 0 16711680) 1 0.835356 2.72478 COMMENT 3.0377) COMMENT #191=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T0 terminator" COMMENT "@N" 1 -6.80204 -12.1963 0 -10 5.74457 COMMENT 1.04901 #190#) COMMENT #192=(CScratch COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #138# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 1 0.183029 1.9 COMMENT 0.082322 1) COMMENT #193=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "end of terminator" "@N" 1 -10.2544 10.454 0 COMMENT -10 7.41799 1.04901 #192#) COMMENT #194=(CLine COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #138# 268632076 COMMENT 100) (LOGPEN 0 0 16711680) 1 0.835356 COMMENT 0.0856105 0.144652) COMMENT #195=(CLabel (CWidget 0 (0 0) 1 2 0 0 #117# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Stem loop" "@N" COMMENT 1 -4.35444 12.1963 0 -10 4.42082 1.04901 COMMENT #194#)) (CObjectList))) (CObjectList)) COMMENT #196=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -530 100) COMMENT (CObjectList COMMENT #197=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -112 100) COMMENT (CObjectList COMMENT #198=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #124# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 6.05178 1.9 COMMENT 0.082322 1) COMMENT #199=(CLabel (CWidget 0 (0 0) 1 2 0 0 #126# 1 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Pst}I (76)" "@N (@S)" 5 10.4702 8.71165 COMMENT 0 -10 2.84731 0.799245 #198#)) (CObjectList)) COMMENT #200=(CGroupWidget COMMENT (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -391 100) COMMENT (CObjectList COMMENT #201=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #124# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 0.0177125 1.9 COMMENT 0.082322 1) COMMENT #202=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #126# 41166252 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Xba}I (2120)" "@N (@S)" 5 -3.60046 COMMENT 15.681 0 -10 3.79641 0.799245 #201#)) COMMENT (CObjectList)) COMMENT #203=(CGroupWidget COMMENT (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -600 100) COMMENT (CObjectList COMMENT #204=(CScratch COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #124# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 0.0619938 1.9 COMMENT 0.082322 1) COMMENT #205=(CLabel (CWidget 0 (0 0) 1 2 0 0 #126# 1 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Eco}RI (2105)" "@N (@S)" 5 -4.25452 COMMENT 13.9386 0 -10 4.14608 0.799245 #204#)) COMMENT (CObjectList)) COMMENT #206=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -182 100) COMMENT (CObjectList COMMENT #207=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #124# 33997448 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 6.10492 1.9 COMMENT 0.082322 1) COMMENT #208=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #126# 34316416 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Ahl}I (58)" "@N (@S)" 5 4.719 12.1963 0 COMMENT -10 2.92224 0.799245 #207#)) (CObjectList))) COMMENT (CObjectList)) COMMENT #209=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 1200 100) COMMENT (CObjectList) (CObjectList)) COMMENT #210=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -670 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 56..76 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 77..148 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 85..119 /vntifkey="39" /label=Stem\loop primer_bind complement(212..231) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(316..930) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 331..331 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1097..1202 /vntifkey="39" /label=T0\terminator CDS complement(1215..1874) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 1988..2007 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2064..2067) /vntifkey="39" /label=end\of\terminator stem_loop complement(2077..2096) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..55 /vntifkey="21" /label=BBa_K608002 promoter 1..35 /vntifkey="30" /label=constitutive\promoter\family\member RBS 44..55 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency BASE COUNT 587 a 515 c 492 g 531 t ORIGIN 1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta 61 gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt 121 taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct 181 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 241 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 301 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc 361 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 421 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 481 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 541 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 601 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 661 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 721 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 781 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 841 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 901 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 961 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 1021 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 1081 ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc 1141 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg 1201 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta 1261 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc 1321 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag 1381 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag 1441 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac 1501 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag 1561 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc 1621 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg 1681 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt 1741 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga 1801 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg 1861 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg 1921 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac 1981 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 2041 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt 2101 ctggaattcg cggccgcttc tagag //