LOCUS pSB1C3 3018 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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"{\\i Ahl}I (951)" "@N (@S)" 5 13.2113 COMMENT -5.22699 0 -10 3.172 0.799245 #224#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #226=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1223 100) COMMENT (CObjectList) (CObjectList)) COMMENT #227=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -874 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 949..969 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 970..1041 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 978..1012 /vntifkey="39" /label=Stem\loop primer_bind complement(1105..1124) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1209..1823) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1224..1224 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1990..2095 /vntifkey="39" /label=T0\terminator CDS complement(2108..2767) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2881..2900 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2957..2960) /vntifkey="39" /label=end\of\terminator stem_loop complement(2970..2989) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..948 /vntifkey="21" /label=BBa_K608351 promoter 1..35 /vntifkey="30" /label=constitutive\promoter\family\member RBS 44..55 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency terminator 797..837 /vntifkey="43" /label=TE\from\coliphage\T7 promoter 900..948 /vntifkey="30" /label=promoter\(lambda\cI\regulated) CDS 62..161 /vntifkey="4" /label=cIts\coding\region BASE COUNT 841 a 698 c 709 g 770 t ORIGIN 1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa 121 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa 181 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc 241 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc 301 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag 361 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag 421 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc 481 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag 541 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt 601 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg 661 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg 721 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gctgactcca 781 gcggccgcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 841 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt ttactagagt 901 aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcta ctagtagcgg 961 ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc 1021 gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt 1081 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 1141 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 1201 aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa 1261 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 1321 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 1381 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 1441 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 1501 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 1561 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 1621 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 1681 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 1741 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 1801 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 1861 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 1921 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 1981 ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa 2041 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga 2101 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc 2161 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca 2221 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca 2281 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa 2341 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat 2401 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg 2461 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca 2521 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa 2581 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg 2641 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct 2701 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt 2761 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta 2821 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg 2881 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 2941 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat 3001 tcgcggccgc ttctagag //