LOCUS pSB1C3 3164 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 3164 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 3164 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1115 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3159 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3144 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 1 1097 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 1095 1115 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 1116 1187 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 1124 1158 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 1251 1270 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 1355 1969 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 1370 1370 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 2136 2241 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 2254 2913 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 3027 3046 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 3103 3106 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 3116 3135 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_K774006" 21 0 0 1 1094 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 217 228 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) COMMENT "engineered cyan fluorescent protein derived from A. victoria GFP" COMMENT 21 0 0 235 957 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #18=(CFSignal (CObList) "T1 from E. coli rrnB" 43 0 0 966 1045 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #19=(CFSignal (CObList) "TE from coliphage T7" 43 0 0 1054 1094 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #20=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #21=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #22=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" COMMENT 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Creation Date: ? (no data)" 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Last Modification Date: ? (no data)" 1) COMMENT #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "length: 3164 bp" 1) COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Basic" 1) COMMENT #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "form: Circular" 1))) "General Description") COMMENT #31=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Original Source Database: GenBank" 1) COMMENT #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Associated Genes: rep (pMB1); ORI; Chloramphenicol resistance" COMMENT 1))) "Standard Fields") COMMENT #34=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" COMMENT 0 0) COMMENT #35=(CFolderPar COMMENT (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList)) COMMENT "Annotations") COMMENT #36=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #37=(CFolderPar COMMENT (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #38=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2254 (3 #11# 0) 1 2 2 0 327) COMMENT (CObjectList COMMENT #39=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2254 End: 2913 (Complementary)" COMMENT 1) COMMENT #40=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #41=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(1160..1819)" 1) COMMENT #42=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #43=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"Chloramphenicol resistance\"" COMMENT 1))) "Chloramphenicol resistance"))) COMMENT "CDS (1 total)") COMMENT #44=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #45=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1 (3 #15# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #46=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1 End: 1094" 1))) COMMENT "BBa_K774006") COMMENT #47=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 235 (3 #17# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #48=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 235 End: 957 " 1))) COMMENT "engineered cyan fluorescent protein derived from A. victoria GFP") COMMENT #49=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1095 (3 #4# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #50=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1095 End: 1115" 1) COMMENT #51=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #52=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1..21" 1))) "DNA: BioBrick suffix") COMMENT #53=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1355 (3 #8# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #54=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1355 End: 1969 (Complementary)" COMMENT 1) COMMENT #55=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #56=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(261..875)" 1) COMMENT #57=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #58=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"rep (pMB1)\"" 1))) COMMENT "rep (pMB1)") COMMENT #59=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1370 (3 #9# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #60=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1370 End: 1370" 1) COMMENT #61=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #62=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 276..276" 1) COMMENT #63=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Qualifiers:" 1) COMMENT #64=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " /gene=\"ORI\"" 1))) "ORI"))) COMMENT "Misc. Feature (5 total)") COMMENT #65=(CFolderPar COMMENT (CGroupPar (CParagraph 28 (7 28 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #66=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1251 (3 #7# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #67=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1251 End: 1270 (Complementary)" COMMENT 1) COMMENT #68=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #69=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(157..176)" 1))) COMMENT "Verification reverse (VR) primer binding site") COMMENT #70=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3027 (3 #12# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #71=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3027 End: 3046" 1) COMMENT #72=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #73=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1933..1952" 1))) COMMENT "Verification forward (VF2) primer binding site"))) COMMENT "Primer Binding Site (2 total)") COMMENT #74=(CFolderPar COMMENT (CGroupPar (CParagraph 32 (7 32 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #75=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 217 (3 #16# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #76=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 217 End: 228 " 1))) COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency"))) COMMENT "RBS (1 total)") COMMENT #77=(CFolderPar COMMENT (CGroupPar (CParagraph 39 (7 39 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #78=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1116 (3 #5# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #79=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1116 End: 1187" 1) COMMENT #80=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #81=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 22..93" 1))) COMMENT "E. coli his operon terminator") COMMENT #82=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1124 (3 #6# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #83=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1124 End: 1158" 1) COMMENT #84=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #85=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 30..64" 1))) "Stem loop") COMMENT #86=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 2136 (3 #10# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #87=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 2136 End: 2241" 1) COMMENT #88=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #89=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 1042..1147" 1))) "T0 terminator") COMMENT #90=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3103 (3 #13# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #91=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3103 End: 3106 (Complementary)" COMMENT 1) COMMENT #92=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #93=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2009..2012)" 1))) COMMENT "end of terminator") COMMENT #94=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 3116 (3 #14# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #95=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 3116 End: 3135 (Complementary)" COMMENT 1) COMMENT #96=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #97=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " complement(2022..2041)" 1))) COMMENT "Stem loop"))) "Stem Loop (5 total)") COMMENT #98=(CFolderPar COMMENT (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #99=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 966 (3 #18# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #100=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 966 End: 1045" 1))) COMMENT "T1 from E. coli rrnB") COMMENT #101=(CFolderPar COMMENT (CGroupPar COMMENT (CParagraph 1054 (3 #19# 0) 1 2 2 0 194) COMMENT (CObjectList COMMENT #102=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 1054 End: 1094" 1))) COMMENT "TE from coliphage T7"))) COMMENT "Terminator (2 total)"))) "Feature Map") COMMENT #103=(CFolderPar COMMENT (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #104=(CFolderPar COMMENT (CGroupPar (CParagraph 21 (0 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #105=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #106=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Start: 0 End: 0 " 1) COMMENT #107=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT "Original Location Description:" 1) COMMENT #108=(CLinePar COMMENT (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " 0..0" 1))) COMMENT "DNA: BioBrick prefix"))) COMMENT "Misc. Feature (1 total)"))) COMMENT "Imported Features Not Shown on Map") COMMENT #109=(CFolderPar COMMENT (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #110=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 38129048 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #111=(CGroupPar COMMENT (CParagraph 0 (10 #3# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #112=(CLinePar COMMENT (CParagraph 0 (1 #3# 1) 1 2 2 0 191) COMMENT " N1: 1097 " 1))))) "AhlI: 1 site") COMMENT 1 5 "ACTAGT" "TGATCA") COMMENT #113=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 31501944 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #114=(CGroupPar COMMENT (CParagraph 0 (10 #2# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #115=(CLinePar COMMENT (CParagraph 0 (1 #2# 1) 1 2 2 0 191) COMMENT " N1: 3144 " 1))))) "EcoRI: 1 site") COMMENT 1 5 "GAATTC" "CTTAAG") COMMENT #116=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 31495048 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #117=(CGroupPar COMMENT (CParagraph 0 (10 #0# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #118=(CLinePar COMMENT (CParagraph 0 (1 #0# 1) 1 2 2 0 191) COMMENT " N1: 1115 " 1))))) "PstI: 1 site") COMMENT 5 1 "CTGCAG" "GACGTC") COMMENT #119=(CRSFolderPar COMMENT (CFolderPar COMMENT (CGroupPar (CParagraph 13691992 (8 0) 1 1 1 0 178) COMMENT (CObjectList COMMENT #120=(CGroupPar COMMENT (CParagraph 0 (10 #1# 0) 1 2 2 0 180) COMMENT (CObjectList COMMENT #121=(CLinePar COMMENT (CParagraph 0 (1 #1# 1) 1 2 2 0 191) COMMENT " N1: 3159 " 1))))) "XbaI: 1 site") COMMENT 1 5 "TCTAGA" "AGATCT"))) COMMENT "Restriction/Methylation Map")))) COMMENT (CGraphView COMMENT (CWStyleSheet COMMENT (CObjectList COMMENT #122=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 13408563) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 5 COMMENT "@N (@S)" 0) COMMENT #123=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 COMMENT "@N" 0) COMMENT #124=(CWidgetStyle "Molecule Label 2" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 0 1 16 COMMENT "@L bp" 0) COMMENT #125=(CWidgetStyle "Molecule Label 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Verdana") 0.833333 0 1 1 COMMENT "@N" 0) COMMENT #126=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 3355545) 1 1 COMMENT (LOGBRUSH 0 6724095 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #127=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 6723840) 1 1 COMMENT (LOGBRUSH 0 10079334 0) 0 0 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #128=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 10079436) 2 1 COMMENT (LOGBRUSH 0 13434879 0) 0 0 1 (LOGSHAPE 10 1 0 0 0)) COMMENT #129=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 6723840) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #130=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 10053171) 8 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #131=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 13395507) 10 0 0 0 1 COMMENT (LOGSHAPE 1 1.9 0 0 0)) COMMENT #132=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 153) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Georgia") 0.555556 128 1 COMMENT 5 "@N (@S)" 0) COMMENT #133=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 COMMENT 1 2 "?" 0) COMMENT #134=(CWidgetStyle "Mark 1" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" COMMENT 0) COMMENT #135=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 16744512) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 8388608 COMMENT 1 65535 "@N (@H)" 0) COMMENT #136=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 COMMENT "@F bp (molecule @L bp)" 0) COMMENT #137=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 COMMENT "Fragment of @N" 0) COMMENT #138=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 2 8388608 5) 0 0 0) COMMENT #139=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 COMMENT 0 0) COMMENT #140=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 COMMENT 0) COMMENT #141=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8 0 0 0 1 COMMENT (LOGSHAPE 7 0.2 3.41182 2.86186 0.609808)) COMMENT #142=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) COMMENT #143=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) COMMENT #144=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) COMMENT #145=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) COMMENT #146=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #147=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) COMMENT #148=(CWidgetStyle "Void" 0 0 0 0 0) COMMENT #149=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 1 3 "@T @N " 0) COMMENT #150=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) COMMENT #151=(CWidgetStyle "Annotation" 0 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 COMMENT 0 0) COMMENT #152=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #153=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 COMMENT (LOGBRUSH 0 16777215 0) 0 0 0) COMMENT #154=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 COMMENT "@N" 0) COMMENT #155=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") COMMENT 0.611111 0 1 65535 "@N" 0) COMMENT #156=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 COMMENT "@N" 0) COMMENT #157=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 3 1 COMMENT (LOGBRUSH 0 16777113 0) 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 COMMENT (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #158=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 COMMENT 1 (LOGSHAPE 9 1 0.8 1.8 0)) COMMENT #159=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 COMMENT 1 1 "@N" 0) COMMENT #160=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 COMMENT (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 COMMENT 5 "@N (@S)" 0) COMMENT #161=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 COMMENT (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 COMMENT "@N (@S)" 0)) 0.164644 1.74233 0.164644 2.53336 COMMENT (70 (CShapeMapEntry 0 "Unique RSite" 1 "Uniq RSite Label") 67 COMMENT (CShapeMapEntry 0 "ORF" 0 "ORF Label")) 40.0378 40.0378 39 39 0.1 COMMENT -2070) 1 1 0 1 1 COMMENT (mapper: 29.8822 -17.8387 40.0378 40.0378 0.01 10 -1 3164 3164 1 0 0) COMMENT #162=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil -1148 100) COMMENT (CObjectList COMMENT #163=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -172 100) COMMENT (CObjectList COMMENT #164=(CAxis COMMENT (CWideLine COMMENT (CWidget 0 (0 0) 1 2 0 0 #128# 38319524 0) COMMENT (LOGPEN 0 0 10079436) 2 (LOGBRUSH 0 13434879 0) 1 COMMENT 6.27617 6.27417 1 0.0214037) 0.198268) COMMENT #165=(CLabel COMMENT (CWidget 1001 (0 0) 1 2 0 0 #125# 38117456 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 58 22 0 0 400 0 0 0 0 3 2 1 34 "Verdana") COMMENT 1.74233 0.833333 0 "pSB1C3" "@N" 1 0 0.871165 0 -10 COMMENT 4.49575 1.39868 Nil) COMMENT #166=(CLabel COMMENT (CWidget 1002 (0 0) 1 2 0 0 #124# 1438159696 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 "Georgia") COMMENT 1.74233 0.555556 0 "3164 bp" "@L bp" 16 0 -0.871165 COMMENT 0 -10.8 2.52262 0.799245 Nil)) (CObjectList)) COMMENT #167=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -102 100) COMMENT (CObjectList COMMENT #168=(CGroupWidget COMMENT (CWidget 4 (7 4 0) 1 2 0 0 Nil -316 100) COMMENT (CObjectList COMMENT #169=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #11# 0) 1 2 0 0 #126# 0 100) COMMENT (LOGPEN 0 0 3355545) 1 COMMENT (LOGBRUSH 0 6724095 0) 1 0.497652 1.80622 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #170=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 41168516 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Chloramphenicol resistance" "@N" 1 -17.1945 COMMENT 5.22699 0 -10 12.2634 1.04901 #169#)) COMMENT (CObjectList)) COMMENT #171=(CGroupWidget COMMENT (CWidget 21 (7 21 0) 1 2 0 0 Nil -1079 100) COMMENT (CObjectList COMMENT #172=(CScratch COMMENT (CWidget 0 (3 #4# 0) 1 2 0 0 #131# 38242992 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 4.10414 1.9 COMMENT 0.082322 1) COMMENT #173=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 41169836 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "DNA: BioBrick suffix" "@N" 1 14.5546 COMMENT -6.96932 0 -10 8.81667 1.04901 #172#) COMMENT #174=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #8# 0) 1 2 0 0 #127# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 2.3693 3.58864 1 COMMENT 0.082322) 0.8 1.8 1) COMMENT #175=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 41168268 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "rep (pMB1)" COMMENT "@N" 1 -5.1006 -12.1963 0 -10 4.77049 1.04901 COMMENT #174#) COMMENT #176=(CScratch COMMENT (CWidget 0 (3 #9# 0) 1 2 0 0 #131# 38128008 COMMENT 100) (LOGPEN 0 8 13395507) 10 1 3.5589 1.9 COMMENT 0.082322 1) COMMENT #177=(CLabel (CWidget 0 (0 0) 1 2 0 0 #123# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "ORI" "@N" 1 COMMENT 6.72817 -17.4233 0 -10 1.67342 1.04901 #176#) COMMENT #178=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #15# 0) 1 2 0 0 #127# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 0.835356 4.10414 COMMENT 6.27319 1 0.082322) 0.8 1.8 0) COMMENT #179=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 268751608 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "BBa K774006" COMMENT "@N" 1 13.8055 5.22699 0 -10 5.91941 1.04901 COMMENT #178#) COMMENT #180=(CWideArrow COMMENT (CWideLine COMMENT (CWidget 0 (3 #17# 0) 1 2 0 0 #127# 0 100) COMMENT (LOGPEN 0 0 6723840) 1 COMMENT (LOGBRUSH 0 10079334 0) 1 4.37576 5.80924 1 COMMENT 0.082322) 0.8 1.8 0) COMMENT #181=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 34571320 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "engineered cyan fluorescent protein derived from A. victoria GFP" COMMENT "@N" 1 25.5023 3.48466 0 -10 28.2983 1.04901 COMMENT #180#)) (CObjectList)) COMMENT #182=(CGroupWidget COMMENT (CWidget 28 (7 28 0) 1 2 0 0 Nil -246 100) COMMENT (CObjectList COMMENT #183=(CScratch COMMENT (CWidget 0 (3 #7# 0) 1 2 0 0 #144# 38127208 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 3.79484 1.9 COMMENT 0.082322 1) COMMENT #184=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 2031547210 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification reverse (VR) primer binding site" COMMENT "@N" 1 17.6623 -15.681 0 -10 19.1319 1.04901 COMMENT #183#) COMMENT #185=(CScratch COMMENT (CWidget 0 (3 #12# 0) 1 2 0 0 #144# 38185152 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.273609 1.9 COMMENT 0.082322 1) COMMENT #186=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 41166896 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "Verification forward (VF2) primer binding site" COMMENT "@N" 1 -18.3804 8.71165 0 -10 19.4816 1.04901 COMMENT #185#)) (CObjectList)) COMMENT #187=(CGroupWidget COMMENT (CWidget 32 (7 32 0) 1 2 0 0 Nil -874 100) COMMENT (CObjectList COMMENT #188=(CScratch COMMENT (CWidget 0 (3 #16# 0) 1 2 0 0 #143# 38199600 COMMENT 100) (LOGPEN 0 6 16711680) 8 1 5.84493 1.9 COMMENT 0.082322 1) COMMENT #189=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 41168044 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT "@N" 1 15.7863 10.454 0 -10 19.032 1.04901 COMMENT #188#)) (CObjectList)) COMMENT #190=(CGroupWidget COMMENT (CWidget 39 (7 39 0) 1 2 0 0 Nil -874 100) COMMENT (CObjectList COMMENT #191=(CLine COMMENT (CWidget 0 (3 #5# 0) 1 2 0 0 #144# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 0.835356 3.91975 COMMENT 4.0625) COMMENT #192=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 268632356 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "E. coli his operon terminator" "@N" 1 COMMENT 16.0255 -13.9386 0 -10 12.3383 1.04901 #191#) COMMENT #193=(CLine COMMENT (CWidget 0 (3 #6# 0) 1 2 0 0 #144# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 0.670712 3.97725 COMMENT 4.04664) COMMENT #194=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 444 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Stem loop" "@N" COMMENT 1 12.1348 -12.1963 0 -10 4.42082 1.04901 COMMENT #193#) COMMENT #195=(CLine COMMENT (CWidget 0 (3 #10# 0) 1 2 0 0 #144# 0 100) COMMENT (LOGPEN 0 0 16711680) 1 0.835356 1.83001 COMMENT 2.04017) COMMENT #196=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 2036724498 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "T0 terminator" COMMENT "@N" 1 -14.4995 -3.48466 0 -10 5.74457 COMMENT 1.04901 #195#) COMMENT #197=(CScratch COMMENT (CWidget 0 (3 #13# 0) 1 2 0 0 #144# 38189472 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.122926 1.9 COMMENT 0.082322 1) COMMENT #198=(CLabel (CWidget 0 (0 0) 1 2 0 0 #123# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "end of terminator" "@N" 1 -10.2544 10.454 0 COMMENT -10 7.41799 1.04901 #197#) COMMENT #199=(CScratch COMMENT (CWidget 0 (3 #14# 0) 1 2 0 0 #144# 38192376 COMMENT 100) (LOGPEN 0 0 16711680) 1 1 0.0971511 1.9 COMMENT 0.082322 1) COMMENT #200=(CLabel (CWidget 0 (0 0) 1 2 0 0 #123# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 "Stem loop" "@N" COMMENT 1 -4.14033 12.1963 0 -10 4.42082 1.04901 COMMENT #199#)) (CObjectList)) COMMENT #201=(CGroupWidget COMMENT (CWidget 43 (7 43 0) 1 2 0 0 Nil -1153 100) COMMENT (CObjectList COMMENT #202=(CLine COMMENT (CWidget 0 (3 #18# 0) 1 2 0 0 #131# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 0.670712 4.20129 COMMENT 4.3599) COMMENT #203=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 268751352 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "T1 from E. coli rrnB" "@N" 1 15.754 -3.48466 COMMENT 0 -10 8.39207 1.04901 #202#) COMMENT #204=(CLine COMMENT (CWidget 0 (3 #19# 0) 1 2 0 0 #131# 0 100) COMMENT (LOGPEN 0 8 13395507) 10 0.670712 4.10414 COMMENT 4.18543) COMMENT #205=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #123# 41166252 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 COMMENT "Arial") 1.74233 0.666667 0 COMMENT "TE from coliphage T7" "@N" 1 15.5827 COMMENT -5.22699 0 -10 9.09141 1.04901 #204#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #206=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -1223 100) COMMENT (CObjectList COMMENT #207=(CGroupWidget COMMENT (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -316 100) COMMENT (CObjectList COMMENT #208=(CScratch COMMENT (CWidget 1 (1 #0# 1) 1 2 0 0 #130# 38250400 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 4.06448 1.9 COMMENT 0.082322 1) COMMENT #209=(CLabel (CWidget 0 (0 0) 1 2 0 0 #132# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Pst}I (1115)" "@N (@S)" 5 12.3144 COMMENT -10.454 0 -10 3.19698 0.799245 #208#)) COMMENT (CObjectList)) COMMENT #210=(CGroupWidget COMMENT (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -386 100) COMMENT (CObjectList COMMENT #211=(CScratch COMMENT (CWidget 1 (1 #1# 1) 1 2 0 0 #130# 38200880 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.0118961 1.9 COMMENT 0.082322 1) COMMENT #212=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #132# 40158012 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Xba}I (3159)" "@N (@S)" 5 -3.49597 COMMENT 15.681 0 -10 3.79641 0.799245 #211#)) COMMENT (CObjectList)) COMMENT #213=(CGroupWidget COMMENT (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -1293 100) COMMENT (CObjectList COMMENT #214=(CScratch COMMENT (CWidget 1 (1 #2# 1) 1 2 0 0 #130# 38200240 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 0.0416362 1.9 COMMENT 0.082322 1) COMMENT #215=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #132# 1460 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Eco}RI (3144)" "@N (@S)" 5 -3.9762 COMMENT 13.9386 0 -10 4.24599 0.799245 #214#)) COMMENT (CObjectList)) COMMENT #216=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -595 100) COMMENT (CObjectList COMMENT #217=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #130# 31538392 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 4.10017 1.9 COMMENT 0.082322 1) COMMENT #218=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #132# 1443102884 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Ahl}I (1097)" "@N (@S)" 5 12.6372 COMMENT -8.71165 0 -10 3.62158 0.799245 #217#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #219=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -525 100) COMMENT (CObjectList) (CObjectList)) COMMENT #220=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1363 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 1095..1115 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 1116..1187 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 1124..1158 /vntifkey="39" /label=Stem\loop primer_bind complement(1251..1270) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1355..1969) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1370..1370 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 2136..2241 /vntifkey="39" /label=T0\terminator CDS complement(2254..2913) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 3027..3046 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(3103..3106) /vntifkey="39" /label=end\of\terminator stem_loop complement(3116..3135) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..1094 /vntifkey="21" /label=BBa_K774006 RBS 217..228 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency misc_feature 235..957 /vntifkey="21" /label=engineered\cyan\fluorescent\protein\derived\from\A.\victoria\GFP terminator 966..1045 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 1054..1094 /vntifkey="43" /label=TE\from\coliphage\T7 BASE COUNT 860 a 827 c 758 g 719 t ORIGIN 1 ccatataagg ccatataagg ccatataagg ccatataagg ccatataagg ccatataagg 61 ccatataagg ccatataagg ccatataagg ggatccaagc ttcatatgtt cccatctata 121 atcctccctg attcttcgct gatatggtgc taaaaagtaa ccaataaatg gtatttaaaa 181 tgcaaattat caggcgtacc ctgaaacgta ctagagaaag aggagaaata ctagatggtg 241 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 301 gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 361 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 421 accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 481 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 541 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 601 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 661 gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc 721 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 781 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 841 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 901 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaataatac 961 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 1021 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 1081 tttctgcgtt tatatactag tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca 1141 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg 1201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 1261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 1321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc 1381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 1441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 1501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 1561 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 1621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 1681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 1741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 1801 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 1861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 1921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 1981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 2041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 2101 atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc 2161 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct 2221 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca 2281 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg 2341 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt 2401 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact 2461 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc 2521 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc 2581 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta 2641 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc 2701 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt 2761 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt 2821 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc 2881 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct 2941 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc 3001 tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga 3061 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag 3121 ctttcgctaa ggatgatttc tggaattcgc ggccgcttct agag //