LOCUS pSB1C3 3060 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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Weiss" 32 0 COMMENT 0 217 231 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) COMMENT "**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)" COMMENT 21 0 0 238 943 0 (CStringList) (CStringList) 1 1 1 1 "")) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #18=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #19=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #20=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. Author: UNKNOWN. 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misc_feature 991..1011 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 1012..1083 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 1020..1054 /vntifkey="39" /label=Stem\loop primer_bind complement(1147..1166) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1251..1865) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1266..1266 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 2032..2137 /vntifkey="39" /label=T0\terminator CDS complement(2150..2809) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2923..2942 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2999..3002) /vntifkey="39" /label=end\of\terminator stem_loop complement(3012..3031) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..990 /vntifkey="21" /label=BBa_K774007 RBS 217..231 /vntifkey="32" /label=RBS.1\(strong)\--\modified\from\R.\Weiss misc_feature 238..943 /vntifkey="21" /label=**highly**\engineered\mutant\of\red\fluorescent\protein\from\Discosoma\striata\(coral) BASE COUNT 866 a 743 c 706 g 745 t ORIGIN 1 ccatataagg ccatataagg ccatataagg ccatataagg ccatataagg ccatataagg 61 ccatataagg ccatataagg ccatataagg ggatccaagc ttcatatgtt cccatctata 121 atcctccctg attcttcgct gatatggtgc taaaaagtaa ccaataaatg gtatttaaaa 181 tgcaaattat caggcgtacc ctgaaacgta ctagagatta aagaggagaa atactagatg 241 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc 301 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc 361 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg 421 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac 481 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac 541 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa 601 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg 661 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc 721 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac 781 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc 841 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac 901 tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga gaaaaaaaaa 961 ccccgcccct gacagggcgg ggtttttttt tactagtagc ggccgctgca gtccggcaaa 1021 aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt 1081 tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 1141 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 1201 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 1261 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 1321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 1381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 1441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 1501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 1561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 1621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 1681 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 1741 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 1801 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 1861 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 1921 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 1981 taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc 2041 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga 2101 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc 2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat 2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat 2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat 2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc 2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta 2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct 2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca 2581 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 2821 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat 2881 ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa 2941 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga 3001 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag //