LOCUS pSB1C3 4228 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 4228 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 4228 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 2179 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 4223 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4208 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 2 38 0 2161 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 2159 2179 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 2180 2251 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 2188 2222 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 2315 2334 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 2419 3033 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 2434 2434 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 3200 3305 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 3318 3977 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 4091 4110 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 4167 4170 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 4180 4199 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_K786003" 21 0 0 1 2158 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "constitutive promoter family member" 30 0 0 1 COMMENT 35 0 (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #17=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #18=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #19=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. 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Ahl}I (38)" "@N (@S)" 5 8.18107 12.1963 COMMENT 0 -10 2.92224 0.799245 #201#) COMMENT #203=(CScratch COMMENT (CWidget 2 (1 #3# 2) 1 2 0 0 #121# 88109271 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 3.06834 1.9 COMMENT 0.082322 1) COMMENT #204=(CLabel (CWidget 0 (0 0) 1 2 0 0 #113# 0 100) COMMENT (LOGPEN 0 0 13408563) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 0 COMMENT "{\\i Ahl}I (2161)" "@N (@S)" 5 -4.13253 COMMENT -15.681 0 -10 3.44674 0.799245 #203#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #205=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -735 100) COMMENT (CObjectList) (CObjectList)) COMMENT #206=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1014 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 2159..2179 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 2180..2251 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 2188..2222 /vntifkey="39" /label=Stem\loop primer_bind complement(2315..2334) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(2419..3033) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 2434..2434 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 3200..3305 /vntifkey="39" /label=T0\terminator CDS complement(3318..3977) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 4091..4110 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(4167..4170) /vntifkey="39" /label=end\of\terminator stem_loop complement(4180..4199) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..2158 /vntifkey="21" /label=BBa_K786003 promoter 1..35 /vntifkey="30" /label=constitutive\promoter\family\member BASE COUNT 993 a 1145 c 1148 g 942 t ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gtgaaagagg agaaatacta 61 gaagcttatg gacgccgtcg caaccgccta cctcggcggc gcggtcgcgc tcatcgtcgg 121 tgtggcgttc gtctggttgc tgtaccggtc gttggatggc tccccgcatc agtcggcgct 181 cgcgccgctg gccatcattc ccgtgttcgc gggcctgtcc tacgtgggga tggcgtacga 241 catcggaacg gtgatcgtaa acgggaacca gatcgtcggg ctgcggtaca tcgactggct 301 cgtgacgacg ccgatcctcg tggggtacgt cggctacgcc gcgggggcgt cccgtcgcag 361 catcatcggt gtgatggtgg cggacgcgct catgatcgcg gtgggcgcgg gggcggtggt 421 gactgacggc acgctcaagt gggcgctgtt cggcgtgtcg tcgatcttcc acctgtcgct 481 gttcgcgtac ctgtacgtga tctttccgcg ggtcgtgccc gacgtgcccg agcagatcgg 541 gctgttcaac ctgctgaaaa accacatcgg gctgctgtgg ttggcgtacc cgctggtgtg 601 gctgttcggc ccggccggca tcggggaggc aacggctgcc ggcgtcgcgc tcacgtacgt 661 gttcttggac gtgctcgcga aggtgccgta cgtgtatttc ttctacgcgc ggcgtcgcgt 721 gttcatgcac tcggagtcgc cgccggctcc cgagcaggcg accgtcgagg cgacggcggc 781 ggacggatcc gcgtcgaacg gcgcgtcggc gatgacaatc gcatgggcgc gccgtcgata 841 cggcgttaaa ctcgggctgg ggtatatcgc caccgccggc ctcctggtcg gggtcggcgt 901 caccacaaac gacgtcccct cgaccattgt cgccggaatc gccgggttac tcacgctcgg 961 atcgatcaac gccgccgaga ccgtcgccag catcaaggag atcgccgcac agaccgagcg 1021 cgtcgccaac gggaacctcg aacaggaggt gacctccacc cgaacggacg agttcggatc 1081 gcttgccgac tccatcgaac agatgcgcca gtcgcttcgt ggcaccgtca ctcatgtccg 1141 cgaaggttca gatgccatct atgccggtac ccgtgaaatt gcggcgggca acaccgatct 1201 ttcctcccgt actgaacagc aggcatccgc gctggaagaa actgccgcca gcatggagca 1261 gctcaccgcg acagtgaagc aaaacgccga taacgcccgc caggcctcgc aactggcgca 1321 aagtgcctcc gacaccgccc agcacggcgg caaagtggtg gatggcgtag tgaaaacgat 1381 gcatgagatc gccgatagtt cgaagaaaat tgccgacatt atcagcgtta tcgacggtat 1441 tgccttccag actaatatcc tcgcgctgaa tgccgcggtt gaagccgcgc gtgcgggtga 1501 acagggccgt ggttttgccg tggtggcggg tgaagtgcgt aatcttgcca gtcgcagcgc 1561 ccaggcggca aaagagatca aagccctcat tgaagactcc gtctcacgcg ttgataccgg 1621 ttcggtgctg gtcgaaagcg ccggggaaac aatgaacaat atcgtcaatg ctgtcactcg 1681 cgtgactgac attatgggcg agattgcatc ggcatcggat gaacagagcc gtggcatcga 1741 tcaagtcgca ttggcggttt cggaaatgga tcgcgtcacg caacagaacg catcgctggt 1801 gcaggaatca gctgccgccg ccgctgcgct ggaagaacag gcgagtcgtt taacgcaagc 1861 agtttccgcg ttccgtctgg cagccagccc actcaccaat aaaccgcaaa caccatcccg 1921 tcctgccagt gagcaaccac cggctcagcc acgactgcga attgctgaac aagatccaaa 1981 ctgggaaaca ttttgacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa 2041 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 2101 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 2161 ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt 2221 ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc 2281 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 2341 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 2401 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc 2461 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 2521 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 2581 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 2641 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 2701 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 2761 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 2821 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 2881 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 2941 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 3001 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 3061 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 3121 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 3181 ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg 3241 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa 3301 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca 3361 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc 3421 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag 3481 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct 3541 gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa 3601 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc 3661 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta 3721 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc 3781 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc 3841 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac 3901 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca 3961 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat 4021 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc 4081 aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 4141 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga 4201 tttctggaat tcgcggccgc ttctagag //