LOCUS pSB1C3 2232 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 2232 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 2232 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 183 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2227 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2212 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 1 165 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 163 183 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 184 255 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 192 226 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 319 338 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 423 1037 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 438 438 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 1204 1309 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 1322 1981 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 2095 2114 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 2171 2174 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 2184 2203 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_K792009" 21 0 0 1 162 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) COMMENT "Kozak sequence from yeast alpha-factor mating pheromone (MFalpha1)" COMMENT 21 0 0 1 21 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) COMMENT "Secretion tag from yeast alpha-factor mating pheromone (MFalpha1)" COMMENT 21 0 0 22 75 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #18=(CFSignal (CObList) COMMENT "Import enhancer - HIV TAT penetratin (trojan peptide)" 21 0 0 76 COMMENT 114 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #19=(CFSignal (CObList) "Histidine rich peptide (His Tag)" 21 0 0 115 COMMENT 162 0 (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #20=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #21=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #22=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. 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0 -10 4.07115 0.799245 #210#)) COMMENT (CObjectList)) COMMENT #212=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -740 100) COMMENT (CObjectList COMMENT #213=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #128# 0 100) COMMENT (LOGPEN 0 6 10053171) 8 1 5.81225 1.9 COMMENT 0.082322 1) COMMENT #214=(CLabel (CWidget 0 (0 0) 1 2 0 0 #130# 0 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Ahl}I (165)" "@N (@S)" 5 10.749 8.71165 COMMENT 0 -10 3.172 0.799245 #213#)) (CObjectList))) COMMENT (CObjectList)) COMMENT #215=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -321 100) COMMENT (CObjectList) (CObjectList)) COMMENT #216=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -182 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 163..183 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 184..255 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 192..226 /vntifkey="39" /label=Stem\loop primer_bind complement(319..338) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(423..1037) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 438..438 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1204..1309 /vntifkey="39" /label=T0\terminator CDS complement(1322..1981) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2095..2114 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2171..2174) /vntifkey="39" /label=end\of\terminator stem_loop complement(2184..2203) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..162 /vntifkey="21" /label=BBa_K792009 misc_feature 1..21 /vntifkey="21" /label=Kozak\sequence\from\yeast\alpha-factor\mating\pheromone\(MFalpha1) misc_feature 22..75 /vntifkey="21" /label=Secretion\tag\from\yeast\alpha-factor\mating\pheromone\(MFalpha1) misc_feature 76..114 /vntifkey="21" /label=Import\enhancer\-\HIV\TAT\penetratin\(trojan\peptide) misc_feature 115..162 /vntifkey="21" /label=Histidine\rich\peptide\(His\Tag) BASE COUNT 625 a 544 c 502 g 561 t ORIGIN 1 ggatccacga ttaaaagaat gagattccca tccattttca ccgctgtttt gttcgccgct 61 tcttctgctt tggctaagct ttatggtaga aaaaagcgta gacaacgtag aagaaagctt 121 cacaaccata atcacaacca caatcataac cacaattaat aatactagta gcggccgctg 181 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag 241 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 421 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg 481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 601 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 721 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 781 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 841 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 901 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 961 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 1021 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 1081 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 1141 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag 1201 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag 1261 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa 1321 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga 1381 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt 1441 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg 1501 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat 1561 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg 1621 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg 1681 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct 1741 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt 1801 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa 1861 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat 1921 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca 1981 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc 2041 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct 2101 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 2161 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg 2221 ccgcttctag ag //