LOCUS pSB1C3 5163 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 5163 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 5163 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 3114 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 5158 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 5143 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 1 3096 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 3094 3114 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 3115 3186 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 3123 3157 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 3250 3269 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 3354 3968 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 3369 3369 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 4135 4240 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 4253 4912 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 5026 5045 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 5102 5105 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 5115 5134 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_K823016" 21 0 0 1 3093 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 1 12 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) COMMENT "lacZ (encoding beta-galactosidase, full-length)" 21 0 0 19 3093 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #18=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #19=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #20=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. 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COMMENT #204=(CGroupWidget COMMENT (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -944 100) COMMENT (CObjectList COMMENT #205=(CScratch COMMENT (CWidget 1 (1 #3# 1) 1 2 0 0 #123# 38554816 COMMENT 100) (LOGPEN 0 6 10053171) 8 1 2.51268 1.9 COMMENT 0.082322 1) COMMENT #206=(CLabel COMMENT (CWidget 0 (0 0) 1 2 0 0 #125# 38259256 100) COMMENT (LOGPEN 0 0 153) 1 COMMENT (LOGFONT 38 14 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Georgia") 1.74233 0.555556 128 COMMENT "{\\i Ahl}I (3096)" "@N (@S)" 5 -10.163 COMMENT -12.1963 0 -10 3.79641 0.799245 #205#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #207=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -874 100) COMMENT (CObjectList) (CObjectList)) COMMENT #208=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -874 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 3094..3114 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 3115..3186 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 3123..3157 /vntifkey="39" /label=Stem\loop primer_bind complement(3250..3269) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(3354..3968) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 3369..3369 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 4135..4240 /vntifkey="39" /label=T0\terminator CDS complement(4253..4912) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 5026..5045 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(5102..5105) /vntifkey="39" /label=end\of\terminator stem_loop complement(5115..5134) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..3093 /vntifkey="21" /label=BBa_K823016 RBS 1..12 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency misc_feature 19..3093 /vntifkey="21" /label=lacZ\(encoding\beta-galactosidase,\full-length) BASE COUNT 1257 a 1348 c 1370 g 1188 t ORIGIN 1 aaagaggaga aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt 61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 181 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg 241 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac 301 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt 361 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg 421 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg 481 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg 541 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac 601 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg 661 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat 721 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac 781 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct 841 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg 901 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt 961 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc 1021 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc 1081 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg 1141 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg 1201 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc 1261 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta 1321 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg 1381 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc 1441 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc 1501 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg 1561 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg 1621 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg 1681 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag 1741 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc 1801 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg 1861 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg 1921 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac 1981 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct 2041 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa 2101 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac 2161 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg 2221 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat 2281 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa 2341 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt 2401 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg 2461 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg 2521 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg 2581 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg 2641 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag 2701 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc 2761 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc 2821 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc 2881 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc 2941 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt 3001 ggtggcgacg actcctggag cccgtcagta tcggcggaat ttcagctgag cgccggtcgc 3061 taccattacc agttggtctg gtgtcaaaaa taatactagt agcggccgct gcagtccggc 3121 aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg 3181 cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 3241 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 3301 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 3361 ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 3421 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 3481 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 3541 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 3601 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 3661 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 3721 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 3781 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 3841 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 3901 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 3961 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 4021 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 4081 ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga ggcttggatt 4141 ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc 4201 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc 4261 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc 4321 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg 4381 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta 4441 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa 4501 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt 4561 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt 4621 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt 4681 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg 4741 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct 4801 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac 4861 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt 4921 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat 4981 tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc tgacgtctaa 5041 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcagaatttc 5101 agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg gccgcttcta 5161 gag //