LOCUS pSB1C3 3657 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 3657 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 3657 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1608 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3652 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3637 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 1 1590 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 1588 1608 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 1609 1680 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 1617 1651 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 1744 1763 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 1848 2462 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 1863 1863 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 2629 2734 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 2747 3406 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 3520 3539 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 3596 3599 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 3609 3628 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_K863000" 21 0 0 1 1587 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "T7 promoter" 30 0 0 1 23 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) COMMENT (CTextView 0 COMMENT #17=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #18=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #19=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. 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0.799245 #200#)) COMMENT (CObjectList))) (CObjectList)) COMMENT #202=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1223 100) COMMENT (CObjectList) (CObjectList)) COMMENT #203=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1153 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 1588..1608 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 1609..1680 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 1617..1651 /vntifkey="39" /label=Stem\loop primer_bind complement(1744..1763) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1848..2462) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1863..1863 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 2629..2734 /vntifkey="39" /label=T0\terminator CDS complement(2747..3406) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 3520..3539 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(3596..3599) /vntifkey="39" /label=end\of\terminator stem_loop complement(3609..3628) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..1587 /vntifkey="21" /label=BBa_K863000 promoter 1..23 /vntifkey="30" /label=T7\promoter BASE COUNT 1056 a 866 c 816 g 919 t ORIGIN 1 taatacgact cactataggg agagaggaga aaaatgaacc tagaaaaatt tgttgacgag 61 ctgccaattc cagaagttgc gaagcccgtc aaaaagaacc caaaacaaac gtattatgaa 121 atcgctatgg aggaggtatt tctaaaagtt catagagatc tgcccccaac caagctatgg 181 acctataatg gcagtttgcc tggtccaacc attcatgcga atcgaaatga aaaagtcaaa 241 gtgaaatgga tgaacaaatt gccacttaag cattttctac cggtcgatca caccattcac 301 gaaggccatc atgatgaacc agaagttaaa accgtcgttc atttacatgg tggcgtcaca 361 ccagcaagca gtgatggcta tccagaggct tggttttcac gagactttga agcaaccggc 421 cccttctttg aacgggaggt gtacgaatac ccaaatcatc agcaagcctg cacattgtgg 481 tatcacgatc atgcgatggc attgacacga ttaaatgtgt atgccggctt agctggattt 541 tatttgatct cagatgcgtt tgaaaagtcg ctagaattac cgaagggtga gtatgatatt 601 ccgctaatga tcatggaccg tacgtttcag gaggatggcg cactgtttta tccaagcagg 661 ccaaacaaca caccagaaga cagtgacata ccagatccgt ctatcgtgcc tttcttttgc 721 ggagaaacca ttttggtcaa tggaaaagta tggccgtatt tagaagtaga gccgcgaaaa 781 tatcgttttc gtattttaaa tgcttccaat acaagaactt acgagctgca tcttgacaac 841 gatgcgacga ttttgcaaat tggatctgat ggcggctttt taccaagacc tgttcaccat 901 caatccttta gcattgctcc tgctgaacga tttgatgtca tcatcgattt ttcagcttac 961 gaaaacaaaa cgatcaccct taaaaataaa gccggctgcg gacaggaagt aaatcctgaa 1021 acagatgcaa acatcatgca atttaaagtc actcgaccgc taaaagggag agcacctaaa 1081 acattacggc ctattttcaa accgcttcca ccacttcggc cttgtcgagc tgataaagag 1141 cgtacgctca ctcttaccgg tacacaggat aaatacggcc gtcctatttt attgctagat 1201 aaccaatttt ggaatgaccc tgtcacggaa aatcctcgtc ttggcagtgt ggaggtttgg 1261 tctatcgtca atccaacaag gggcacacat cctattcatt tacaccttgt tcaattcaga 1321 gtgatagaca gaagaccatt tgatactgag gtctatcaat cgacagggga cattgtgtat 1381 acaggaccaa acgaagcacc tcccttacat gaacaaggct acaaggacac cattcaagcg 1441 catgccggtg aagtcattcg gatcatcgct cgctttgttc catacagcgg caggtatgtg 1501 tggcattgtc atatattaga gcacgaggat tatgacatga tgcggccgat ggatatcatc 1561 cagcatcacc atcaccatca ctaataatac tagtagcggc cgctgcagtc cggcaaaaaa 1621 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat 1681 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 1741 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 1801 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 1861 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 1921 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 1981 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 2041 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 2101 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 2161 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 2221 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 2281 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 2341 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 2401 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 2461 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 2521 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 2581 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc 2641 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt 2701 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct 2761 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac 2821 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat 2881 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa 2941 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt 3001 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa 3061 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat 3121 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg 3181 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat 3241 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg 3301 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc 3361 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag 3421 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt 3481 gaaagttgga acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc 3541 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa 3601 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagag //