LOCUS pSB1C3 3021 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList))) (CObjectList)) COMMENT #200=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -456 100) COMMENT (CObjectList) (CObjectList)) COMMENT #201=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1223 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 2037630044 COMMENT (CStringList) 2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 952..972 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 973..1044 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 981..1015 /vntifkey="39" /label=Stem\loop primer_bind complement(1108..1127) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1212..1826) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1227..1227 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 1993..2098 /vntifkey="39" /label=T0\terminator CDS complement(2111..2770) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 2884..2903 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(2960..2963) /vntifkey="39" /label=end\of\terminator stem_loop complement(2973..2992) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..951 /vntifkey="21" /label=BBa_K936013 misc_feature 1..66 /vntifkey="21" /label=PelB\leader\sequence;\directs\protein\to\E.\coli\periplasmic\membrane BASE COUNT 731 a 843 c 773 g 674 t ORIGIN 1 atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg 61 atggccatgg acggagttct ctggcgagtg cgaaccgcgg cgctcatggc cgcgctgctc 121 gccctcgcag cctgggcgct ggtctgggct tcgcccagcg tcgaggctca atccaacccg 181 taccagcgcg gtcccaaccc cacgcggagc gcgctcacgg ccgacgggcc gttctcggtg 241 gcaacctaca ccgtttcgcg gctctcggtg agcggcttcg ggggcggggt gatctactac 301 cccacaggca cctcgctgac cttcggcggg atcgccatgt cgccggggta cacggccgac 361 gccagttcgc tggcgtggct cgggcggcgg ttggcatcgc acggcttcgt ggtgctcgtc 421 atcaacacga actcgcgatt cgactaccct gactcccgcg caagccagct atcggcggcg 481 ctgaactacc tgcggacgag cagcccctca gccgtacgcg cccggctcga tgcgaaccgc 541 ctggccgttg cggggcattc gatgggcggc ggcgggaccc tccgcatcgc agagcagaac 601 ccgtcgctga aagcggccgt accgctcacg ccgtggcaca ccgacaagac gttcaacacg 661 tcggtaccgg tgctcatcgt gggagcggaa gcggacacgg tcgcgcccgt gagccagcac 721 gccattccgt tctaccagaa cttgccctcg accacgccga aggtgtacgt ggagctcgac 781 aatgcgtcgc acttcgcgcc caacagcaac aacgcggcga tctccgtgta caccatctcg 841 tggatgaagc tgtgggtgga taacgacacc cgctaccggc agttcctctg caacgtgaac 901 gatccggcgc tgagcgactt ccggacgaac aaccgccact gccagtaata atactagtag 961 cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa 1021 accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt 1081 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 1141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 1201 taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa 1261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 1321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 1381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 1441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 1501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 1561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 1621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 1681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 1741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 1801 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 1861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 1921 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 1981 cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa 2041 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct 2101 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca 2161 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca 2221 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt 2281 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga 2341 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca 2401 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg 2461 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata 2521 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg 2581 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa 2641 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg 2701 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt 2761 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg 2821 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaactcga 2881 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 2941 atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg 3001 aattcgcggc cgcttctaga g //