LOCUS pSB1C3 3315 bp DNA circular DEFINITION High copy BioBrick assembly plasmid. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTNAME|pSB1C3| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pSB1C3" 0 3315 COMMENT (CDBMol 0 0 1 1 1 0 0 1667457377 0 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList) (CObList) -1) COMMENT (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 10 4294967295 50 0 COMMENT 1 0 (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) COMMENT (CStringList "PstI" "XbaI" "EcoRI" "AhlI") (CStringList "atg" "gtg") COMMENT (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 COMMENT 4294967295 0 1 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 70 20 1 1 1 1 0 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 0 1 3315 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #0=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1266 0 0 "") COMMENT #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3310 0 0 "") COMMENT #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3295 0 0 "") COMMENT #3=(CRSite (CStringList) "AhlI" "actagt" 2 0 1 1248 0 0 "")) COMMENT (CObList COMMENT #4=(CFSignal (CObList) "DNA: BioBrick suffix" 21 0 0 1246 1266 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "1..21") COMMENT #5=(CFSignal (CObList) "E. coli his operon terminator" 39 0 0 1267 1338 COMMENT 0 (CStringList) (CStringList) 1 1 1 1 "22..93") COMMENT #6=(CFSignal (CObList) "Stem loop" 39 0 0 1275 1309 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "30..64") COMMENT #7=(CFSignal (CObList) "Verification reverse (VR) primer binding site" COMMENT 28 0 1 1402 1421 0 (CStringList) (CStringList) 1 1 1 1 COMMENT "complement(157..176)") COMMENT #8=(CFSignal (CObList) "rep (pMB1)" 21 0 1 1506 2120 0 (CStringList) COMMENT (CStringList "/gene=\"rep (pMB1)\"") 1 1 1 1 "complement(261..875)") COMMENT #9=(CFSignal (CObList) "ORI" 21 0 0 1521 1521 0 (CStringList) COMMENT (CStringList "/gene=\"ORI\"") 1 1 1 1 "276..276") COMMENT #10=(CFSignal (CObList) "T0 terminator" 39 0 0 2287 2392 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1042..1147") COMMENT #11=(CFSignal (CObList) "Chloramphenicol resistance" 4 0 1 2405 3064 0 COMMENT (CStringList) (CStringList "/gene=\"Chloramphenicol resistance\"") COMMENT 1 1 1 1 "complement(1160..1819)") COMMENT #12=(CFSignal (CObList) "Verification forward (VF2) primer binding site" COMMENT 28 0 0 3178 3197 0 (CStringList) (CStringList) 1 1 1 1 "1933..1952") COMMENT #13=(CFSignal (CObList) "end of terminator" 39 0 1 3254 3257 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2009..2012)") COMMENT #14=(CFSignal (CObList) "Stem loop" 39 0 1 3267 3286 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(2022..2041)") COMMENT #15=(CFSignal (CObList) "BBa_S04617" 21 0 0 1 1245 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "") COMMENT #16=(CFSignal (CObList) "RBS (Elowitz 1999) -- defines RBS efficiency" COMMENT 32 0 0 259 270 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #17=(CFSignal (CObList) "cI repressor from E. coli phage lambda (+LVA)" COMMENT 21 0 0 277 1051 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #18=(CFSignal (CObList) "T1 from E. coli rrnB" 43 0 0 1060 1139 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #19=(CFSignal (CObList) "TE from coliphage T7" 43 0 0 1148 1188 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "") COMMENT #20=(CFSignal (CObList) "promoter (lambda cI regulated)" 29 0 0 1197 COMMENT 1245 0 (CStringList) (CStringList) 1 1 1 1 "") COMMENT #21=(CFSignal (CObList) "FixK2 promoter" 30 0 0 1 250 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "1..250")) (CObList) (CObList) (CObList) COMMENT (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #23=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pSB1C3" 2) 5 "" 0 COMMENT 4) COMMENT #24=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pSB1C3'" 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "High copy BioBrick assembly plasmid." 1) COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "Foreign object. 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2037630016 2147541141 (CObList))) FEATURES Location/Qualifiers misc_feature 1246..1266 /vntifkey="21" /label=DNA:\BioBrick\suffix stem_loop 1267..1338 /vntifkey="39" /label=E.\coli\his\operon\terminator stem_loop 1275..1309 /vntifkey="39" /label=Stem\loop primer_bind complement(1402..1421) /vntifkey="28" /label=Verification\reverse\(VR)\primer\binding\site misc complement(1506..2120) /gene="rep (pMB1)" /vntifkey="21" /label=rep\(pMB1) /note="Unknown feature type:misc" misc 1521..1521 /gene="ORI" /vntifkey="21" /label=ORI /note="Unknown feature type:misc" stem_loop 2287..2392 /vntifkey="39" /label=T0\terminator CDS complement(2405..3064) /gene="Chloramphenicol resistance" /vntifkey="4" /label=Chloramphenicol\resistance primer_bind 3178..3197 /vntifkey="28" /label=Verification\forward\(VF2)\primer\binding\site stem_loop complement(3254..3257) /vntifkey="39" /label=end\of\terminator stem_loop complement(3267..3286) /vntifkey="39" /label=Stem\loop misc_feature 0..0 /vntifkey="21" /label=DNA:\BioBrick\prefix misc_feature 1..1245 /vntifkey="21" /label=BBa_S04617 promoter 1..250 /vntifkey="30" /label=FixK2\promoter RBS 259..270 /vntifkey="32" /label=RBS\(Elowitz\1999)\--\defines\RBS\efficiency misc_feature 277..1051 /vntifkey="21" /label=cI\repressor\from\E.\coli\phage\lambda\(+LVA) terminator 1060..1139 /vntifkey="43" /label=T1\from\E.\coli\rrnB terminator 1148..1188 /vntifkey="43" /label=TE\from\coliphage\T7 promoter 1197..1245 /vntifkey="29" /label=promoter\(lambda\cI\regulated) BASE COUNT 902 a 799 c 784 g 830 t ORIGIN 1 gccggagccg attatccgca cccgtccttg gtcttggaca cactcgctcg cgatccgaag 61 ccgcctttca aagatactcc gcagtgaaac gcatctttta agcgcgactt gtcgccttcg 121 cgcagcagaa cactcgtatg gcactcaatc catgatcgct tcgttcgctc cgacgcgcgt 181 tgcggggccc ccctcgaccg gatgacaaca cattgcgtca cctcaactgc tgcgcgcgca 241 ctgcgtcaca tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 301 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 361 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 421 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 481 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 541 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 601 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 661 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 721 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 781 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 841 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 901 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 961 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 1021 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 1081 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1141 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac 1201 accgtgcgtg ttgactattt tacctctggc ggtgataatg gttgctacta gtagcggccg 1261 ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa 1321 aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg 1381 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 1441 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 1501 ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg 1561 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 1621 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 1681 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 1741 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 1801 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 1861 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 1921 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 1981 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 2041 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 2101 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 2161 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 2221 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc 2281 gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc 2341 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat 2401 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc 2461 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct 2521 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat 2581 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca 2641 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt 2701 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa 2761 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca 2821 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa 2881 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg 2941 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa 3001 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct 3061 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg 3121 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca 3181 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 3241 aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt ctggaattcg 3301 cggccgcttc tagag //